Identification and Characterization of msf, a Novel Virulence Factor in Haemophilus influenzae.
Haemophilus influenzae is an opportunistic pathogen. The emergence of virulent, non-typeable strains (NTHi) emphasizes the importance of developing new interventional targets. We screened the NTHi supragenome for genes encoding surface-exposed proteins suggestive of immune evasion, identifying a lar...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2016-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4792463?pdf=render |
| _version_ | 1818285404203253760 |
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| author | Jennifer M Kress-Bennett N Luisa Hiller Rory A Eutsey Evan Powell Mark J Longwell Todd Hillman Tenisha Blackwell Barbara Byers Joshua C Mell J Christopher Post Fen Z Hu Garth D Ehrlich Benjamin A Janto |
| author_facet | Jennifer M Kress-Bennett N Luisa Hiller Rory A Eutsey Evan Powell Mark J Longwell Todd Hillman Tenisha Blackwell Barbara Byers Joshua C Mell J Christopher Post Fen Z Hu Garth D Ehrlich Benjamin A Janto |
| author_sort | Jennifer M Kress-Bennett |
| collection | DOAJ |
| description | Haemophilus influenzae is an opportunistic pathogen. The emergence of virulent, non-typeable strains (NTHi) emphasizes the importance of developing new interventional targets. We screened the NTHi supragenome for genes encoding surface-exposed proteins suggestive of immune evasion, identifying a large family containing Sel1-like repeats (SLRs). Clustering identified ten SLR-containing gene subfamilies, each with various numbers of SLRs per gene. Individual strains also had varying numbers of SLR-containing genes from one or more of the subfamilies. Statistical genetic analyses of gene possession among 210 NTHi strains typed as either disease or carriage found a significant association between possession of the SlrVA subfamily (which we have termed, macrophage survival factor, msf) and the disease isolates. The PittII strain contains four chromosomally contiguous msf genes. Deleting all four of these genes (msfA1-4) (KO) resulted in a highly significant decrease in phagocytosis and survival in macrophages; which was fully complemented by a single copy of the msfA1 gene. Using the chinchilla model of otitis media and invasive disease, the KO strain displayed a significant decrease in fitness compared to the WT in co-infections; and in single infections, the KO lost its ability to invade the brain. The singly complemented strain showed only a partial ability to compete with the WT suggesting gene dosage is important in vivo. The transcriptional profiles of the KO and WT in planktonic growth were compared using the NTHi supragenome array, which revealed highly significant changes in the expression of operons involved in virulence and anaerobiosis. These findings demonstrate that the msfA1-4 genes are virulence factors for phagocytosis, persistence, and trafficking to non-mucosal sites. |
| first_indexed | 2024-12-13T01:08:09Z |
| format | Article |
| id | doaj.art-f1c74fde6f4d48cc9828dd599740cd3b |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-13T01:08:09Z |
| publishDate | 2016-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-f1c74fde6f4d48cc9828dd599740cd3b2022-12-22T00:04:31ZengPublic Library of Science (PLoS)PLoS ONE1932-62032016-01-01113e014989110.1371/journal.pone.0149891Identification and Characterization of msf, a Novel Virulence Factor in Haemophilus influenzae.Jennifer M Kress-BennettN Luisa HillerRory A EutseyEvan PowellMark J LongwellTodd HillmanTenisha BlackwellBarbara ByersJoshua C MellJ Christopher PostFen Z HuGarth D EhrlichBenjamin A JantoHaemophilus influenzae is an opportunistic pathogen. The emergence of virulent, non-typeable strains (NTHi) emphasizes the importance of developing new interventional targets. We screened the NTHi supragenome for genes encoding surface-exposed proteins suggestive of immune evasion, identifying a large family containing Sel1-like repeats (SLRs). Clustering identified ten SLR-containing gene subfamilies, each with various numbers of SLRs per gene. Individual strains also had varying numbers of SLR-containing genes from one or more of the subfamilies. Statistical genetic analyses of gene possession among 210 NTHi strains typed as either disease or carriage found a significant association between possession of the SlrVA subfamily (which we have termed, macrophage survival factor, msf) and the disease isolates. The PittII strain contains four chromosomally contiguous msf genes. Deleting all four of these genes (msfA1-4) (KO) resulted in a highly significant decrease in phagocytosis and survival in macrophages; which was fully complemented by a single copy of the msfA1 gene. Using the chinchilla model of otitis media and invasive disease, the KO strain displayed a significant decrease in fitness compared to the WT in co-infections; and in single infections, the KO lost its ability to invade the brain. The singly complemented strain showed only a partial ability to compete with the WT suggesting gene dosage is important in vivo. The transcriptional profiles of the KO and WT in planktonic growth were compared using the NTHi supragenome array, which revealed highly significant changes in the expression of operons involved in virulence and anaerobiosis. These findings demonstrate that the msfA1-4 genes are virulence factors for phagocytosis, persistence, and trafficking to non-mucosal sites.http://europepmc.org/articles/PMC4792463?pdf=render |
| spellingShingle | Jennifer M Kress-Bennett N Luisa Hiller Rory A Eutsey Evan Powell Mark J Longwell Todd Hillman Tenisha Blackwell Barbara Byers Joshua C Mell J Christopher Post Fen Z Hu Garth D Ehrlich Benjamin A Janto Identification and Characterization of msf, a Novel Virulence Factor in Haemophilus influenzae. PLoS ONE |
| title | Identification and Characterization of msf, a Novel Virulence Factor in Haemophilus influenzae. |
| title_full | Identification and Characterization of msf, a Novel Virulence Factor in Haemophilus influenzae. |
| title_fullStr | Identification and Characterization of msf, a Novel Virulence Factor in Haemophilus influenzae. |
| title_full_unstemmed | Identification and Characterization of msf, a Novel Virulence Factor in Haemophilus influenzae. |
| title_short | Identification and Characterization of msf, a Novel Virulence Factor in Haemophilus influenzae. |
| title_sort | identification and characterization of msf a novel virulence factor in haemophilus influenzae |
| url | http://europepmc.org/articles/PMC4792463?pdf=render |
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