IFNγ-INDUCED KINURENINE PRODUCTION AND INDOLAMINE 2,3-DIOXYGENASE GENE EXPRESSION IN PSORIASIS
Abstract. An alternative pathway of L-tryptophan biotransformation is provided by the indolamine 2,3-dioxygenase (INDO), and it results into synthesis of kynurenine and other «distal» metabolites, playing a pivotal role in immunoregulation and down-regulation of immune inflammation. PBMCs from healt...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | Russian |
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St. Petersburg branch of the Russian Association of Allergologists and Clinical Immunologists
2014-07-01
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| Series: | Медицинская иммунология |
| Subjects: | |
| Online Access: | https://www.mimmun.ru/mimmun/article/view/277 |
| _version_ | 1797198127148564480 |
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| author | D. A. Kaut R. S. Yamidanov S. V. Sadovnikov O. M. Kapuler V. A. Vakhitov S. V. Sibiryak |
| author_facet | D. A. Kaut R. S. Yamidanov S. V. Sadovnikov O. M. Kapuler V. A. Vakhitov S. V. Sibiryak |
| author_sort | D. A. Kaut |
| collection | DOAJ |
| description | Abstract. An alternative pathway of L-tryptophan biotransformation is provided by the indolamine 2,3-dioxygenase (INDO), and it results into synthesis of kynurenine and other «distal» metabolites, playing a pivotal role in immunoregulation and down-regulation of immune inflammation. PBMCs from healthy volunteers, patients with progressive vulgar psoriasis (PASI (M±SD) = 25.6±16.6), or patients with psoriatic arthropathy (PASI = 40.3±24.5). The cells were incubated for 24 hrs with IFNγ (500 IU/ml, stimulated cultures) or without cytokine (control cultures). Distinct abnormalities in spontaneous and induced kynurenine production (colorimetric method) and INDO expression (semi-quantitative RT-PCR) were observed in psoriasis and psoriatic arthritis. PBMCs from psoriatic patients, in comparison with healthy donors, were characterized with slightly increased spontaneous kynurenine production, spontaneous and IFNγ-induced INDO expression, while IFNγ-induced kynurenine levels were approximately two times higher. In psoriatic arthritis, spontaneous kynurenine production and INDO expression were significantly lower than in donor’s PBMC, whereas IFNγ-induced kynurenine production were the same as for the donors, while IFNγ – induced INDO expression was markedly increased. |
| first_indexed | 2024-03-08T05:52:09Z |
| format | Article |
| id | doaj.art-f1c87023d4414481bbd8cb99a6c90be0 |
| institution | Directory Open Access Journal |
| issn | 1563-0625 2313-741X |
| language | Russian |
| last_indexed | 2024-04-24T06:54:55Z |
| publishDate | 2014-07-01 |
| publisher | St. Petersburg branch of the Russian Association of Allergologists and Clinical Immunologists |
| record_format | Article |
| series | Медицинская иммунология |
| spelling | doaj.art-f1c87023d4414481bbd8cb99a6c90be02024-04-22T13:07:35ZrusSt. Petersburg branch of the Russian Association of Allergologists and Clinical ImmunologistsМедицинская иммунология1563-06252313-741X2014-07-01112-314715210.15789/1563-0625-2009-2-3-147-152274IFNγ-INDUCED KINURENINE PRODUCTION AND INDOLAMINE 2,3-DIOXYGENASE GENE EXPRESSION IN PSORIASISD. A. Kaut0R. S. Yamidanov1S. V. Sadovnikov2O. M. Kapuler3V. A. Vakhitov4S. V. Sibiryak5Институт биохимии и генетики Уфимского научного центра РАН, г. Уфа; Институт иммунологии и физиологии УрО РАН, г. ЕкатеринбургИнститут биохимии и генетики Уфимского научного центра РАН, г. УфаИнститут биохимии и генетики Уфимского научного центра РАН, г. УфаИнститут иммунологии и физиологии УрО РАН, г. ЕкатеринбургИнститут биохимии и генетики Уфимского научного центра РАН, г. УфаИнститут биохимии и генетики Уфимского научного центра РАН, г. Уфа; Институт иммунологии и физиологии УрО РАН, г. ЕкатеринбургAbstract. An alternative pathway of L-tryptophan biotransformation is provided by the indolamine 2,3-dioxygenase (INDO), and it results into synthesis of kynurenine and other «distal» metabolites, playing a pivotal role in immunoregulation and down-regulation of immune inflammation. PBMCs from healthy volunteers, patients with progressive vulgar psoriasis (PASI (M±SD) = 25.6±16.6), or patients with psoriatic arthropathy (PASI = 40.3±24.5). The cells were incubated for 24 hrs with IFNγ (500 IU/ml, stimulated cultures) or without cytokine (control cultures). Distinct abnormalities in spontaneous and induced kynurenine production (colorimetric method) and INDO expression (semi-quantitative RT-PCR) were observed in psoriasis and psoriatic arthritis. PBMCs from psoriatic patients, in comparison with healthy donors, were characterized with slightly increased spontaneous kynurenine production, spontaneous and IFNγ-induced INDO expression, while IFNγ-induced kynurenine levels were approximately two times higher. In psoriatic arthritis, spontaneous kynurenine production and INDO expression were significantly lower than in donor’s PBMC, whereas IFNγ-induced kynurenine production were the same as for the donors, while IFNγ – induced INDO expression was markedly increased.https://www.mimmun.ru/mimmun/article/view/277ifnγkinurenineindolamine 2,3-dioxygenasepsoriasis |
| spellingShingle | D. A. Kaut R. S. Yamidanov S. V. Sadovnikov O. M. Kapuler V. A. Vakhitov S. V. Sibiryak IFNγ-INDUCED KINURENINE PRODUCTION AND INDOLAMINE 2,3-DIOXYGENASE GENE EXPRESSION IN PSORIASIS Медицинская иммунология ifnγ kinurenine indolamine 2,3-dioxygenase psoriasis |
| title | IFNγ-INDUCED KINURENINE PRODUCTION AND INDOLAMINE 2,3-DIOXYGENASE GENE EXPRESSION IN PSORIASIS |
| title_full | IFNγ-INDUCED KINURENINE PRODUCTION AND INDOLAMINE 2,3-DIOXYGENASE GENE EXPRESSION IN PSORIASIS |
| title_fullStr | IFNγ-INDUCED KINURENINE PRODUCTION AND INDOLAMINE 2,3-DIOXYGENASE GENE EXPRESSION IN PSORIASIS |
| title_full_unstemmed | IFNγ-INDUCED KINURENINE PRODUCTION AND INDOLAMINE 2,3-DIOXYGENASE GENE EXPRESSION IN PSORIASIS |
| title_short | IFNγ-INDUCED KINURENINE PRODUCTION AND INDOLAMINE 2,3-DIOXYGENASE GENE EXPRESSION IN PSORIASIS |
| title_sort | ifnγ induced kinurenine production and indolamine 2 3 dioxygenase gene expression in psoriasis |
| topic | ifnγ kinurenine indolamine 2,3-dioxygenase psoriasis |
| url | https://www.mimmun.ru/mimmun/article/view/277 |
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