Characterisation of an atypical manifestation of black band disease on Porites lutea in the Western Indian Ocean
Recent surveys conducted on Reunion Island coral reefs revealed an atypical manifestation of black band disease on the main framework building coral, Porites lutea. This BBD manifestation (PorBBD) presented a thick lighter-colored band, which preceded the typical BBD lesion. Whilst BBD aetiology has...
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PeerJ Inc.
2016-07-01
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| author | Mathieu Séré David A. Wilkinson Michael H. Schleyer Pascale Chabanet Jean-Pascal Quod Pablo Tortosa |
| author_facet | Mathieu Séré David A. Wilkinson Michael H. Schleyer Pascale Chabanet Jean-Pascal Quod Pablo Tortosa |
| author_sort | Mathieu Séré |
| collection | DOAJ |
| description | Recent surveys conducted on Reunion Island coral reefs revealed an atypical manifestation of black band disease on the main framework building coral, Porites lutea. This BBD manifestation (PorBBD) presented a thick lighter-colored band, which preceded the typical BBD lesion. Whilst BBD aetiology has been intensively described worldwide, it remains unclear if corals with apparently similar lesions across coral reefs are affected by the same pathogens. Therefore, a multidisciplinary approach involving field surveys, gross lesion monitoring, histopathology and 454-pyrosequencing was employed to provide the first comprehensive characterization of this particular manifestation. Surveys conducted within two geomorphological zones over two consecutive summers and winters showed spatial and seasonal patterns consistent with those found for typical BBD. Genetic analyses suggested an uncharacteristically high level of Vibrio spp. bacterial infection within PorBBD. However, microscopic analysis revealed high densities of cyanobacteria, penetrating the compromised tissue as well as the presence of basophilic bodies resembling bacterial aggregates in the living tissue, adjacent to the bacterial mat. Additionally, classical BBD-associated cyanobacterial strains, genetically related to Pseudoscillatoria coralii and Roseofilum reptotaenium were identified and isolated and the presence of sulfate-reducers or sulfide-oxidizers such as Desulfovibrio and Arcobacter, previously shown to be associated with anoxic microenvironment within typical BBD was also observed, confirming that PorBBD is a manifestation of classical BBD. |
| first_indexed | 2024-03-09T08:01:08Z |
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| institution | Directory Open Access Journal |
| issn | 2167-8359 |
| language | English |
| last_indexed | 2024-03-09T08:01:08Z |
| publishDate | 2016-07-01 |
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| spelling | doaj.art-f1cb1a9d3e0546d4898d03121200440c2023-12-03T00:46:33ZengPeerJ Inc.PeerJ2167-83592016-07-014e207310.7717/peerj.2073Characterisation of an atypical manifestation of black band disease on Porites lutea in the Western Indian OceanMathieu Séré0David A. Wilkinson1Michael H. Schleyer2Pascale Chabanet3Jean-Pascal Quod4Pablo Tortosa5Agence pour la Recherche et la VAlorisation Marines (ARVAM), Saint-Denis, Réunion, FranceUnité Mixte de Recherche “Processus Infectieux en Milieu Insulaire Tropical” (UMR PIMIT), Université de La Réunion, Inserm1187, CNRS9192, IRD249, Plateforme de Recherche CYROI, Saint Denis, Réunion, FranceOceanographic Research Institute (ORI), Durban, KwaZulu-Natal, South AfricaUMR ENTROPIE, Labex CORAIL, Research Institute for the Development (IRD), Saint-Denis, Réunion, FranceAgence pour la Recherche et la VAlorisation Marines (ARVAM), Saint-Denis, Réunion, FranceUnité Mixte de Recherche “Processus Infectieux en Milieu Insulaire Tropical” (UMR PIMIT), Université de La Réunion, Inserm1187, CNRS9192, IRD249, Plateforme de Recherche CYROI, Saint Denis, Réunion, FranceRecent surveys conducted on Reunion Island coral reefs revealed an atypical manifestation of black band disease on the main framework building coral, Porites lutea. This BBD manifestation (PorBBD) presented a thick lighter-colored band, which preceded the typical BBD lesion. Whilst BBD aetiology has been intensively described worldwide, it remains unclear if corals with apparently similar lesions across coral reefs are affected by the same pathogens. Therefore, a multidisciplinary approach involving field surveys, gross lesion monitoring, histopathology and 454-pyrosequencing was employed to provide the first comprehensive characterization of this particular manifestation. Surveys conducted within two geomorphological zones over two consecutive summers and winters showed spatial and seasonal patterns consistent with those found for typical BBD. Genetic analyses suggested an uncharacteristically high level of Vibrio spp. bacterial infection within PorBBD. However, microscopic analysis revealed high densities of cyanobacteria, penetrating the compromised tissue as well as the presence of basophilic bodies resembling bacterial aggregates in the living tissue, adjacent to the bacterial mat. Additionally, classical BBD-associated cyanobacterial strains, genetically related to Pseudoscillatoria coralii and Roseofilum reptotaenium were identified and isolated and the presence of sulfate-reducers or sulfide-oxidizers such as Desulfovibrio and Arcobacter, previously shown to be associated with anoxic microenvironment within typical BBD was also observed, confirming that PorBBD is a manifestation of classical BBD.https://peerj.com/articles/2073.pdfBacterial communitiesCoral diseaseMetabarcodingPorites black band diseaseReunion IslandScleractinian corals |
| spellingShingle | Mathieu Séré David A. Wilkinson Michael H. Schleyer Pascale Chabanet Jean-Pascal Quod Pablo Tortosa Characterisation of an atypical manifestation of black band disease on Porites lutea in the Western Indian Ocean PeerJ Bacterial communities Coral disease Metabarcoding Porites black band disease Reunion Island Scleractinian corals |
| title | Characterisation of an atypical manifestation of black band disease on Porites lutea in the Western Indian Ocean |
| title_full | Characterisation of an atypical manifestation of black band disease on Porites lutea in the Western Indian Ocean |
| title_fullStr | Characterisation of an atypical manifestation of black band disease on Porites lutea in the Western Indian Ocean |
| title_full_unstemmed | Characterisation of an atypical manifestation of black band disease on Porites lutea in the Western Indian Ocean |
| title_short | Characterisation of an atypical manifestation of black band disease on Porites lutea in the Western Indian Ocean |
| title_sort | characterisation of an atypical manifestation of black band disease on porites lutea in the western indian ocean |
| topic | Bacterial communities Coral disease Metabarcoding Porites black band disease Reunion Island Scleractinian corals |
| url | https://peerj.com/articles/2073.pdf |
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