Altered Expression of GDF9 and BMP15 Genes in Granulosa Cells of Diminished Ovarian Reserve Patients: A Case-Control Study
Objective: Diminished ovarian reserve (DOR) is a challenging issue encountered during assisted reproductive technology. Growth differentiation factor 9 (GDF9) and bone morphogenetic protein 15 (BMP15) belong to the transforming growth factor-beta (TGF-β) superfamily which are essential for folliculo...
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| Format: | Article |
| Language: | English |
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Royan Institute (ACECR), Tehran
2022-09-01
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| Series: | Cell Journal |
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| Online Access: | https://www.celljournal.org/article_253332_17577a2a90d28c9688e6d340f794f9b9.pdf |
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| author | Maryam Omrizadeh Pegah Mokhtari Poopak Eftekhari-Yazdi Zahra Chekini Anahita Mohseni Meybodi |
| author_facet | Maryam Omrizadeh Pegah Mokhtari Poopak Eftekhari-Yazdi Zahra Chekini Anahita Mohseni Meybodi |
| author_sort | Maryam Omrizadeh |
| collection | DOAJ |
| description | Objective: Diminished ovarian reserve (DOR) is a challenging issue encountered during assisted reproductive technology. Growth differentiation factor 9 (GDF9) and bone morphogenetic protein 15 (BMP15) belong to the transforming growth factor-beta (TGF-β) superfamily which are essential for folliculogenesis. We aimed to the evaluationof the GDF9 and BMP15 expression in the granulosa cells (GCs) of DOR patients.Materials and Methods: This case-control study included 14 women with DOR and 12 controls, who were between 28-40 years of age undergoing controlled ovarian stimulation with a gonadotropin releasing hormone (GnRH) antagonist protocol. DOR patients were selected by the Bologna criteria. The GCs were extracted from the aspirated follicular fluids and RNA isolated from this. The fold change of gene expressions was assessed by real-time polymerase chain reaction (PCR).Results: GDF9 expression in patients was 0.23 times lower than the control group, which was significant (P<0.0001). BMP15 expression in patients was 0.32 times lower than the control group, which was significant (P<0.0001). The number of archived oocytes, MII, and two pronuclei (PN) embryos was higher in the control group and these differences were statistically significant (P<0.05).Conclusion: Given that GDF9 and BMP15 are specifically involved during follicular recruitmen., we expect expression of these two genes in DOR patients which is greatly reduced by reducing follicular reserve. |
| first_indexed | 2024-04-12T04:32:59Z |
| format | Article |
| id | doaj.art-f1ce1dcff6494315811b501c6743af74 |
| institution | Directory Open Access Journal |
| issn | 2228-5806 2228-5814 |
| language | English |
| last_indexed | 2024-04-12T04:32:59Z |
| publishDate | 2022-09-01 |
| publisher | Royan Institute (ACECR), Tehran |
| record_format | Article |
| series | Cell Journal |
| spelling | doaj.art-f1ce1dcff6494315811b501c6743af742022-12-22T03:47:53ZengRoyan Institute (ACECR), TehranCell Journal2228-58062228-58142022-09-0124954054510.22074/cellj.2022.8077253332Altered Expression of GDF9 and BMP15 Genes in Granulosa Cells of Diminished Ovarian Reserve Patients: A Case-Control StudyMaryam Omrizadeh0Pegah Mokhtari1Poopak Eftekhari-Yazdi2Zahra Chekini3Anahita Mohseni Meybodi4Department of Medical Genetics, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, IranDepartment of Genetics, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran,IranDepartment of Embryology, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, IranDepartment of Endocrinology and Female Infertility, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, IranDepartment of Genetics, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, IranObjective: Diminished ovarian reserve (DOR) is a challenging issue encountered during assisted reproductive technology. Growth differentiation factor 9 (GDF9) and bone morphogenetic protein 15 (BMP15) belong to the transforming growth factor-beta (TGF-β) superfamily which are essential for folliculogenesis. We aimed to the evaluationof the GDF9 and BMP15 expression in the granulosa cells (GCs) of DOR patients.Materials and Methods: This case-control study included 14 women with DOR and 12 controls, who were between 28-40 years of age undergoing controlled ovarian stimulation with a gonadotropin releasing hormone (GnRH) antagonist protocol. DOR patients were selected by the Bologna criteria. The GCs were extracted from the aspirated follicular fluids and RNA isolated from this. The fold change of gene expressions was assessed by real-time polymerase chain reaction (PCR).Results: GDF9 expression in patients was 0.23 times lower than the control group, which was significant (P<0.0001). BMP15 expression in patients was 0.32 times lower than the control group, which was significant (P<0.0001). The number of archived oocytes, MII, and two pronuclei (PN) embryos was higher in the control group and these differences were statistically significant (P<0.05).Conclusion: Given that GDF9 and BMP15 are specifically involved during follicular recruitmen., we expect expression of these two genes in DOR patients which is greatly reduced by reducing follicular reserve.https://www.celljournal.org/article_253332_17577a2a90d28c9688e6d340f794f9b9.pdfbone morphogenetic protein 15growth differentiation factor 9ovarian reserve |
| spellingShingle | Maryam Omrizadeh Pegah Mokhtari Poopak Eftekhari-Yazdi Zahra Chekini Anahita Mohseni Meybodi Altered Expression of GDF9 and BMP15 Genes in Granulosa Cells of Diminished Ovarian Reserve Patients: A Case-Control Study Cell Journal bone morphogenetic protein 15 growth differentiation factor 9 ovarian reserve |
| title | Altered Expression of GDF9 and BMP15 Genes in Granulosa Cells of Diminished Ovarian Reserve Patients: A Case-Control Study |
| title_full | Altered Expression of GDF9 and BMP15 Genes in Granulosa Cells of Diminished Ovarian Reserve Patients: A Case-Control Study |
| title_fullStr | Altered Expression of GDF9 and BMP15 Genes in Granulosa Cells of Diminished Ovarian Reserve Patients: A Case-Control Study |
| title_full_unstemmed | Altered Expression of GDF9 and BMP15 Genes in Granulosa Cells of Diminished Ovarian Reserve Patients: A Case-Control Study |
| title_short | Altered Expression of GDF9 and BMP15 Genes in Granulosa Cells of Diminished Ovarian Reserve Patients: A Case-Control Study |
| title_sort | altered expression of gdf9 and bmp15 genes in granulosa cells of diminished ovarian reserve patients a case control study |
| topic | bone morphogenetic protein 15 growth differentiation factor 9 ovarian reserve |
| url | https://www.celljournal.org/article_253332_17577a2a90d28c9688e6d340f794f9b9.pdf |
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