Detection of Dinophysistoxin-1 in Clonal Culture of Marine Dinoflagellate Prorocentrum foraminosum (Faust M.A., 1993) from the Sea of Japan
For the first time the presence of dinophysistoxin-1 (DTX-1) in a culture of Prorocentrum foraminosum was revealed in cells and in the culture medium. The clone was isolated from coastal waters of the Sea of Japan and identified by molecular analyses of SSU and D1/D2 regions of LSU rDNA. The concent...
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MDPI AG
2015-09-01
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| Series: | Toxins |
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| Online Access: | http://www.mdpi.com/2072-6651/7/10/3947 |
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| author | Polina A. Kameneva Kseniya V. Efimova Viacheslav G. Rybin Tatiana Y. Orlova |
| author_facet | Polina A. Kameneva Kseniya V. Efimova Viacheslav G. Rybin Tatiana Y. Orlova |
| author_sort | Polina A. Kameneva |
| collection | DOAJ |
| description | For the first time the presence of dinophysistoxin-1 (DTX-1) in a culture of Prorocentrum foraminosum was revealed in cells and in the culture medium. The clone was isolated from coastal waters of the Sea of Japan and identified by molecular analyses of SSU and D1/D2 regions of LSU rDNA. The concentration of DTX-1 in cells was 8.4 ± 2.5 pg/cell and, in cell-free media, 27.9 ± 14.7 µg/L. The toxin presence was confirmed by HPLC with high-resolution tandem mass-spectrometry. |
| first_indexed | 2024-04-11T20:47:43Z |
| format | Article |
| id | doaj.art-f1ce6fcdada44549a233bd035f90cbb6 |
| institution | Directory Open Access Journal |
| issn | 2072-6651 |
| language | English |
| last_indexed | 2024-04-11T20:47:43Z |
| publishDate | 2015-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Toxins |
| spelling | doaj.art-f1ce6fcdada44549a233bd035f90cbb62022-12-22T04:03:59ZengMDPI AGToxins2072-66512015-09-017103947395910.3390/toxins7103947toxins7103947Detection of Dinophysistoxin-1 in Clonal Culture of Marine Dinoflagellate Prorocentrum foraminosum (Faust M.A., 1993) from the Sea of JapanPolina A. Kameneva0Kseniya V. Efimova1Viacheslav G. Rybin2Tatiana Y. Orlova3Zhirmunsky Institute of Marine Biology of the Far Eastern Branch of the Russian Academy of Sciences, Palchevskogo St., 17, Vladivostok 690041, RussiaZhirmunsky Institute of Marine Biology of the Far Eastern Branch of the Russian Academy of Sciences, Palchevskogo St., 17, Vladivostok 690041, RussiaZhirmunsky Institute of Marine Biology of the Far Eastern Branch of the Russian Academy of Sciences, Palchevskogo St., 17, Vladivostok 690041, RussiaZhirmunsky Institute of Marine Biology of the Far Eastern Branch of the Russian Academy of Sciences, Palchevskogo St., 17, Vladivostok 690041, RussiaFor the first time the presence of dinophysistoxin-1 (DTX-1) in a culture of Prorocentrum foraminosum was revealed in cells and in the culture medium. The clone was isolated from coastal waters of the Sea of Japan and identified by molecular analyses of SSU and D1/D2 regions of LSU rDNA. The concentration of DTX-1 in cells was 8.4 ± 2.5 pg/cell and, in cell-free media, 27.9 ± 14.7 µg/L. The toxin presence was confirmed by HPLC with high-resolution tandem mass-spectrometry.http://www.mdpi.com/2072-6651/7/10/3947Prorocentrum foraminosumDTX-1molecular identificationHPLC-HRMSRussia |
| spellingShingle | Polina A. Kameneva Kseniya V. Efimova Viacheslav G. Rybin Tatiana Y. Orlova Detection of Dinophysistoxin-1 in Clonal Culture of Marine Dinoflagellate Prorocentrum foraminosum (Faust M.A., 1993) from the Sea of Japan Toxins Prorocentrum foraminosum DTX-1 molecular identification HPLC-HRMS Russia |
| title | Detection of Dinophysistoxin-1 in Clonal Culture of Marine Dinoflagellate Prorocentrum foraminosum (Faust M.A., 1993) from the Sea of Japan |
| title_full | Detection of Dinophysistoxin-1 in Clonal Culture of Marine Dinoflagellate Prorocentrum foraminosum (Faust M.A., 1993) from the Sea of Japan |
| title_fullStr | Detection of Dinophysistoxin-1 in Clonal Culture of Marine Dinoflagellate Prorocentrum foraminosum (Faust M.A., 1993) from the Sea of Japan |
| title_full_unstemmed | Detection of Dinophysistoxin-1 in Clonal Culture of Marine Dinoflagellate Prorocentrum foraminosum (Faust M.A., 1993) from the Sea of Japan |
| title_short | Detection of Dinophysistoxin-1 in Clonal Culture of Marine Dinoflagellate Prorocentrum foraminosum (Faust M.A., 1993) from the Sea of Japan |
| title_sort | detection of dinophysistoxin 1 in clonal culture of marine dinoflagellate prorocentrum foraminosum faust m a 1993 from the sea of japan |
| topic | Prorocentrum foraminosum DTX-1 molecular identification HPLC-HRMS Russia |
| url | http://www.mdpi.com/2072-6651/7/10/3947 |
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