Applications of tandem mass spectrometry (MS/MS) in antimicrobial peptides field: Current state and new applications
Antimicrobial peptides (AMPs) constitute a group of small molecular peptides that exhibit a wide range of antimicrobial activity. These peptides are abundantly present in the innate immune system of various organisms. Given the rise of multidrug-resistant bacteria, microbiological studies have ident...
| Main Authors: | , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2024-04-01
|
| Series: | Heliyon |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844024045158 |
| _version_ | 1797221801911123968 |
|---|---|
| author | Panpan Fang Songlin Yu Xiaoli Ma Lian Hou Tiewei Li Kaijie Gao Yingyuan Wang Qianqian Sun Lujun Shang Qianqian Liu Manjie Nie Junmei Yang |
| author_facet | Panpan Fang Songlin Yu Xiaoli Ma Lian Hou Tiewei Li Kaijie Gao Yingyuan Wang Qianqian Sun Lujun Shang Qianqian Liu Manjie Nie Junmei Yang |
| author_sort | Panpan Fang |
| collection | DOAJ |
| description | Antimicrobial peptides (AMPs) constitute a group of small molecular peptides that exhibit a wide range of antimicrobial activity. These peptides are abundantly present in the innate immune system of various organisms. Given the rise of multidrug-resistant bacteria, microbiological studies have identified AMPs as potential natural antibiotics. In the context of antimicrobial resistance across various human pathogens, AMPs hold considerable promise for clinical applications. However, numerous challenges exist in the detection of AMPs, particularly by immunological and molecular biological methods, especially when studying of newly discovered AMPs in proteomics. This review outlines the current status of AMPs research and the strategies employed in their development, considering resent discoveries and methodologies. Subsequently, we focus on the advanced techniques of mass spectrometry for the quantification of AMPs in diverse samples, and analyzes their application, advantages, and limitations. Additionally, we propose suggestions for the future development of tandem mass spectrometry for the detection of AMPs. |
| first_indexed | 2024-04-24T13:11:13Z |
| format | Article |
| id | doaj.art-f1cf720c7965492283b60397247d6f39 |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-04-24T13:11:13Z |
| publishDate | 2024-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj.art-f1cf720c7965492283b60397247d6f392024-04-05T04:41:08ZengElsevierHeliyon2405-84402024-04-01107e28484Applications of tandem mass spectrometry (MS/MS) in antimicrobial peptides field: Current state and new applicationsPanpan Fang0Songlin Yu1Xiaoli Ma2Lian Hou3Tiewei Li4Kaijie Gao5Yingyuan Wang6Qianqian Sun7Lujun Shang8Qianqian Liu9Manjie Nie10Junmei Yang11Zhengzhou Key Laboratory of Children's Infection and Immunity, Children's Hospital Affiliated to Zhengzhou University, Henan Children's Hospital, Zhengzhou Children's Hospital, Zhengzhou, 450018, PR ChinaDepartment of Laboratory Medicine, Peking Union Medical College Hospital, Peking Union Medical College & Chinese Academy of Medical Sciences, Beijing, 100730, PR ChinaDepartment of Laboratory Medicine, Peking Union Medical College Hospital, Peking Union Medical College & Chinese Academy of Medical Sciences, Beijing, 100730, PR ChinaDepartment of Laboratory Medicine, Peking Union Medical College Hospital, Peking Union Medical College & Chinese Academy of Medical Sciences, Beijing, 100730, PR ChinaZhengzhou Key Laboratory of Children's Infection and Immunity, Children's Hospital Affiliated to Zhengzhou University, Henan Children's Hospital, Zhengzhou Children's Hospital, Zhengzhou, 450018, PR ChinaZhengzhou Key Laboratory of Children's Infection and Immunity, Children's Hospital Affiliated to Zhengzhou University, Henan Children's Hospital, Zhengzhou Children's Hospital, Zhengzhou, 450018, PR ChinaDepartment of Neonatal Intensive Care Unit, Children's Hospital Affiliated to Zhengzhou University, Henan Children's Hospital, Zhengzhou Children's Hospital, Zhengzhou, 450018, PR ChinaZhengzhou Key Laboratory of Children's Infection and Immunity, Children's Hospital Affiliated to Zhengzhou University, Henan Children's Hospital, Zhengzhou Children's Hospital, Zhengzhou, 450018, PR ChinaDepartment of Laboratory Medicine, Guizhou Provincial People's Hospital, Guiyang, 550004, PR ChinaZhengzhou Key Laboratory of Children's Infection and Immunity, Children's Hospital Affiliated to Zhengzhou University, Henan Children's Hospital, Zhengzhou Children's Hospital, Zhengzhou, 450018, PR ChinaZhengzhou Key Laboratory of Children's Infection and Immunity, Children's Hospital Affiliated to Zhengzhou University, Henan Children's Hospital, Zhengzhou Children's Hospital, Zhengzhou, 450018, PR ChinaZhengzhou Key Laboratory of Children's Infection and Immunity, Children's Hospital Affiliated to Zhengzhou University, Henan Children's Hospital, Zhengzhou Children's Hospital, Zhengzhou, 450018, PR China; Corresponding author. Zhengzhou Key Laboratory of Children's Infection and Immunity, Children's Hospital Affiliated to Zhengzhou University, Henan Children's Hospital, Zhengzhou Children's Hospital, No. 1 Waihuan East Road, Longhu, Zhengzhou, 450018, PR China.Antimicrobial peptides (AMPs) constitute a group of small molecular peptides that exhibit a wide range of antimicrobial activity. These peptides are abundantly present in the innate immune system of various organisms. Given the rise of multidrug-resistant bacteria, microbiological studies have identified AMPs as potential natural antibiotics. In the context of antimicrobial resistance across various human pathogens, AMPs hold considerable promise for clinical applications. However, numerous challenges exist in the detection of AMPs, particularly by immunological and molecular biological methods, especially when studying of newly discovered AMPs in proteomics. This review outlines the current status of AMPs research and the strategies employed in their development, considering resent discoveries and methodologies. Subsequently, we focus on the advanced techniques of mass spectrometry for the quantification of AMPs in diverse samples, and analyzes their application, advantages, and limitations. Additionally, we propose suggestions for the future development of tandem mass spectrometry for the detection of AMPs.http://www.sciencedirect.com/science/article/pii/S2405844024045158Mass spectrometryLC-MS/MSAntimicrobial peptidesApplicationsExtraction |
| spellingShingle | Panpan Fang Songlin Yu Xiaoli Ma Lian Hou Tiewei Li Kaijie Gao Yingyuan Wang Qianqian Sun Lujun Shang Qianqian Liu Manjie Nie Junmei Yang Applications of tandem mass spectrometry (MS/MS) in antimicrobial peptides field: Current state and new applications Heliyon Mass spectrometry LC-MS/MS Antimicrobial peptides Applications Extraction |
| title | Applications of tandem mass spectrometry (MS/MS) in antimicrobial peptides field: Current state and new applications |
| title_full | Applications of tandem mass spectrometry (MS/MS) in antimicrobial peptides field: Current state and new applications |
| title_fullStr | Applications of tandem mass spectrometry (MS/MS) in antimicrobial peptides field: Current state and new applications |
| title_full_unstemmed | Applications of tandem mass spectrometry (MS/MS) in antimicrobial peptides field: Current state and new applications |
| title_short | Applications of tandem mass spectrometry (MS/MS) in antimicrobial peptides field: Current state and new applications |
| title_sort | applications of tandem mass spectrometry ms ms in antimicrobial peptides field current state and new applications |
| topic | Mass spectrometry LC-MS/MS Antimicrobial peptides Applications Extraction |
| url | http://www.sciencedirect.com/science/article/pii/S2405844024045158 |
| work_keys_str_mv | AT panpanfang applicationsoftandemmassspectrometrymsmsinantimicrobialpeptidesfieldcurrentstateandnewapplications AT songlinyu applicationsoftandemmassspectrometrymsmsinantimicrobialpeptidesfieldcurrentstateandnewapplications AT xiaolima applicationsoftandemmassspectrometrymsmsinantimicrobialpeptidesfieldcurrentstateandnewapplications AT lianhou applicationsoftandemmassspectrometrymsmsinantimicrobialpeptidesfieldcurrentstateandnewapplications AT tieweili applicationsoftandemmassspectrometrymsmsinantimicrobialpeptidesfieldcurrentstateandnewapplications AT kaijiegao applicationsoftandemmassspectrometrymsmsinantimicrobialpeptidesfieldcurrentstateandnewapplications AT yingyuanwang applicationsoftandemmassspectrometrymsmsinantimicrobialpeptidesfieldcurrentstateandnewapplications AT qianqiansun applicationsoftandemmassspectrometrymsmsinantimicrobialpeptidesfieldcurrentstateandnewapplications AT lujunshang applicationsoftandemmassspectrometrymsmsinantimicrobialpeptidesfieldcurrentstateandnewapplications AT qianqianliu applicationsoftandemmassspectrometrymsmsinantimicrobialpeptidesfieldcurrentstateandnewapplications AT manjienie applicationsoftandemmassspectrometrymsmsinantimicrobialpeptidesfieldcurrentstateandnewapplications AT junmeiyang applicationsoftandemmassspectrometrymsmsinantimicrobialpeptidesfieldcurrentstateandnewapplications |