Master regulators of skeletal muscle lineage development and pluripotent stem cells differentiation
Abstract In vertebrates, the skeletal muscles of the body and their associated stem cells originate from muscle progenitor cells, during development. The specification of the muscles of the trunk, head and limbs, relies on the activity of distinct genetic hierarchies. The major regulators of trunk a...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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SpringerOpen
2021-10-01
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| Series: | Cell Regeneration |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s13619-021-00093-5 |
| _version_ | 1819117891063119872 |
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| author | Joana Esteves de Lima Frédéric Relaix |
| author_facet | Joana Esteves de Lima Frédéric Relaix |
| author_sort | Joana Esteves de Lima |
| collection | DOAJ |
| description | Abstract In vertebrates, the skeletal muscles of the body and their associated stem cells originate from muscle progenitor cells, during development. The specification of the muscles of the trunk, head and limbs, relies on the activity of distinct genetic hierarchies. The major regulators of trunk and limb muscle specification are the paired-homeobox transcription factors PAX3 and PAX7. Distinct gene regulatory networks drive the formation of the different muscles of the head. Despite the redeployment of diverse upstream regulators of muscle progenitor differentiation, the commitment towards the myogenic fate requires the expression of the early myogenic regulatory factors MYF5, MRF4, MYOD and the late differentiation marker MYOG. The expression of these genes is activated by muscle progenitors throughout development, in several waves of myogenic differentiation, constituting the embryonic, fetal and postnatal phases of muscle growth. In order to achieve myogenic cell commitment while maintaining an undifferentiated pool of muscle progenitors, several signaling pathways regulate the switch between proliferation and differentiation of myoblasts. The identification of the gene regulatory networks operating during myogenesis is crucial for the development of in vitro protocols to differentiate pluripotent stem cells into myoblasts required for regenerative medicine. |
| first_indexed | 2024-12-22T05:40:11Z |
| format | Article |
| id | doaj.art-f1d2ce55935d4ebc826605e73df24570 |
| institution | Directory Open Access Journal |
| issn | 2045-9769 |
| language | English |
| last_indexed | 2024-12-22T05:40:11Z |
| publishDate | 2021-10-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Cell Regeneration |
| spelling | doaj.art-f1d2ce55935d4ebc826605e73df245702022-12-21T18:37:14ZengSpringerOpenCell Regeneration2045-97692021-10-0110111310.1186/s13619-021-00093-5Master regulators of skeletal muscle lineage development and pluripotent stem cells differentiationJoana Esteves de Lima0Frédéric Relaix1Univ Paris Est Creteil, INSERM, EnvA, EFS, AP-HP, IMRBUniv Paris Est Creteil, INSERM, EnvA, EFS, AP-HP, IMRBAbstract In vertebrates, the skeletal muscles of the body and their associated stem cells originate from muscle progenitor cells, during development. The specification of the muscles of the trunk, head and limbs, relies on the activity of distinct genetic hierarchies. The major regulators of trunk and limb muscle specification are the paired-homeobox transcription factors PAX3 and PAX7. Distinct gene regulatory networks drive the formation of the different muscles of the head. Despite the redeployment of diverse upstream regulators of muscle progenitor differentiation, the commitment towards the myogenic fate requires the expression of the early myogenic regulatory factors MYF5, MRF4, MYOD and the late differentiation marker MYOG. The expression of these genes is activated by muscle progenitors throughout development, in several waves of myogenic differentiation, constituting the embryonic, fetal and postnatal phases of muscle growth. In order to achieve myogenic cell commitment while maintaining an undifferentiated pool of muscle progenitors, several signaling pathways regulate the switch between proliferation and differentiation of myoblasts. The identification of the gene regulatory networks operating during myogenesis is crucial for the development of in vitro protocols to differentiate pluripotent stem cells into myoblasts required for regenerative medicine.https://doi.org/10.1186/s13619-021-00093-5MyogenesisMuscle progenitorPAX3PAX7MRFMYF5 |
| spellingShingle | Joana Esteves de Lima Frédéric Relaix Master regulators of skeletal muscle lineage development and pluripotent stem cells differentiation Cell Regeneration Myogenesis Muscle progenitor PAX3 PAX7 MRF MYF5 |
| title | Master regulators of skeletal muscle lineage development and pluripotent stem cells differentiation |
| title_full | Master regulators of skeletal muscle lineage development and pluripotent stem cells differentiation |
| title_fullStr | Master regulators of skeletal muscle lineage development and pluripotent stem cells differentiation |
| title_full_unstemmed | Master regulators of skeletal muscle lineage development and pluripotent stem cells differentiation |
| title_short | Master regulators of skeletal muscle lineage development and pluripotent stem cells differentiation |
| title_sort | master regulators of skeletal muscle lineage development and pluripotent stem cells differentiation |
| topic | Myogenesis Muscle progenitor PAX3 PAX7 MRF MYF5 |
| url | https://doi.org/10.1186/s13619-021-00093-5 |
| work_keys_str_mv | AT joanaestevesdelima masterregulatorsofskeletalmusclelineagedevelopmentandpluripotentstemcellsdifferentiation AT fredericrelaix masterregulatorsofskeletalmusclelineagedevelopmentandpluripotentstemcellsdifferentiation |