New trends in liquid chromatography and their utilization in analysis of beer and brewery raw materials. Part 3. Comparison of HPLC and UHPLC determination of α- and β-acids.
The traditional method of High Performance Liquid Chromatography (HPLC) was compared with the currently increasingly used Ultra- High Performance Liquid Chromatography (UHPLC) on a representative group of 11 hop samples. The results of this work confirmed that the UHPLC technique ensures high throug...
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| Format: | Article |
| Language: | ces |
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Research Institute of Brewing and Malting, Plc.
2012-06-01
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| Series: | Kvasný průmysl |
| Subjects: | |
| Online Access: | https://kvasnyprumysl.cz/en/artkey/kpr-201206-0001_Nove_trendy_v_kapalinove_chromatografii_a_jejich_vyuziti_v_analyze_piva_a_pivovarskych_surovin_Cast_3_Porovna.php |
| _version_ | 1818408345211502592 |
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| author | Marie JURKOVÁ Pavel ČEJKA Jana OLŠOVSKÁ |
| author_facet | Marie JURKOVÁ Pavel ČEJKA Jana OLŠOVSKÁ |
| author_sort | Marie JURKOVÁ |
| collection | DOAJ |
| description | The traditional method of High Performance Liquid Chromatography (HPLC) was compared with the currently increasingly used Ultra- High Performance Liquid Chromatography (UHPLC) on a representative group of 11 hop samples. The results of this work confirmed that the UHPLC technique ensures high throughput also in determination of α- and β-acids. Another benefit of this method is the decrease of result uncertainty which is determined by high separation properties of the UHPLC system. While original HPLC method originating from Analytica EBC 7.7 provides 4 separate peaks (co-elution of n-adhumulone and adhumulone and also of n-lupulone and adlupulone), all 6 analyzed bitter compounds were completely separated using UHPLC.The traditional method of High Performance Liquid Chromatography (HPLC) was compared with the currently increasingly used Ultra- High Performance Liquid Chromatography (UHPLC) on a representative group of 11 hop samples. The results of this work confirmed that the UHPLC technique ensures high throughput also in determination of α- and β-acids. Another benefit of this method is the decrease of result uncertainty which is determined by high separation properties of the UHPLC system. While original HPLC method originating from Analytica EBC 7.7 provides 4 separate peaks (co-elution of n-adhumulone and adhumulone and also of n-lupulone and adlupulone), all 6 analyzed bitter compounds were completely separated using UHPLC. |
| first_indexed | 2024-12-14T09:42:15Z |
| format | Article |
| id | doaj.art-f1da1ec895df4103a9812ed594b790b0 |
| institution | Directory Open Access Journal |
| issn | 0023-5830 2570-8619 |
| language | ces |
| last_indexed | 2024-12-14T09:42:15Z |
| publishDate | 2012-06-01 |
| publisher | Research Institute of Brewing and Malting, Plc. |
| record_format | Article |
| series | Kvasný průmysl |
| spelling | doaj.art-f1da1ec895df4103a9812ed594b790b02022-12-21T23:07:46ZcesResearch Institute of Brewing and Malting, Plc.Kvasný průmysl0023-58302570-86192012-06-0158616617010.18832/kp2012016kpr-201206-0001New trends in liquid chromatography and their utilization in analysis of beer and brewery raw materials. Part 3. Comparison of HPLC and UHPLC determination of α- and β-acids.Marie JURKOVÁ0Pavel ČEJKA1Jana OLŠOVSKÁ2Výzkumný ústav pivovarský a sladařský, a.s., Pivovarský ústav Praha, Lípová 15, 120 44 Praha 2Výzkumný ústav pivovarský a sladařský, a.s., Pivovarský ústav Praha, Lípová 15, 120 44 Praha 2Výzkumný ústav pivovarský a sladařský, a.s., Pivovarský ústav Praha, Lípová 15, 120 44 Praha 2The traditional method of High Performance Liquid Chromatography (HPLC) was compared with the currently increasingly used Ultra- High Performance Liquid Chromatography (UHPLC) on a representative group of 11 hop samples. The results of this work confirmed that the UHPLC technique ensures high throughput also in determination of α- and β-acids. Another benefit of this method is the decrease of result uncertainty which is determined by high separation properties of the UHPLC system. While original HPLC method originating from Analytica EBC 7.7 provides 4 separate peaks (co-elution of n-adhumulone and adhumulone and also of n-lupulone and adlupulone), all 6 analyzed bitter compounds were completely separated using UHPLC.The traditional method of High Performance Liquid Chromatography (HPLC) was compared with the currently increasingly used Ultra- High Performance Liquid Chromatography (UHPLC) on a representative group of 11 hop samples. The results of this work confirmed that the UHPLC technique ensures high throughput also in determination of α- and β-acids. Another benefit of this method is the decrease of result uncertainty which is determined by high separation properties of the UHPLC system. While original HPLC method originating from Analytica EBC 7.7 provides 4 separate peaks (co-elution of n-adhumulone and adhumulone and also of n-lupulone and adlupulone), all 6 analyzed bitter compounds were completely separated using UHPLC.https://kvasnyprumysl.cz/en/artkey/kpr-201206-0001_Nove_trendy_v_kapalinove_chromatografii_a_jejich_vyuziti_v_analyze_piva_a_pivovarskych_surovin_Cast_3_Porovna.phpUHPLC - Ultra High Performance Liquid ChromatographyHPLC - High Performance Liquid Chromatographyα-acidsβ- acids |
| spellingShingle | Marie JURKOVÁ Pavel ČEJKA Jana OLŠOVSKÁ New trends in liquid chromatography and their utilization in analysis of beer and brewery raw materials. Part 3. Comparison of HPLC and UHPLC determination of α- and β-acids. Kvasný průmysl UHPLC - Ultra High Performance Liquid Chromatography HPLC - High Performance Liquid Chromatography α-acids β- acids |
| title | New trends in liquid chromatography and their utilization in analysis of beer and brewery raw materials. Part 3. Comparison of HPLC and UHPLC determination of α- and β-acids. |
| title_full | New trends in liquid chromatography and their utilization in analysis of beer and brewery raw materials. Part 3. Comparison of HPLC and UHPLC determination of α- and β-acids. |
| title_fullStr | New trends in liquid chromatography and their utilization in analysis of beer and brewery raw materials. Part 3. Comparison of HPLC and UHPLC determination of α- and β-acids. |
| title_full_unstemmed | New trends in liquid chromatography and their utilization in analysis of beer and brewery raw materials. Part 3. Comparison of HPLC and UHPLC determination of α- and β-acids. |
| title_short | New trends in liquid chromatography and their utilization in analysis of beer and brewery raw materials. Part 3. Comparison of HPLC and UHPLC determination of α- and β-acids. |
| title_sort | new trends in liquid chromatography and their utilization in analysis of beer and brewery raw materials part 3 comparison of hplc and uhplc determination of α and β acids |
| topic | UHPLC - Ultra High Performance Liquid Chromatography HPLC - High Performance Liquid Chromatography α-acids β- acids |
| url | https://kvasnyprumysl.cz/en/artkey/kpr-201206-0001_Nove_trendy_v_kapalinove_chromatografii_a_jejich_vyuziti_v_analyze_piva_a_pivovarskych_surovin_Cast_3_Porovna.php |
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