Protective effect of oil from Cornus wilsoniana fruits against carbon tetrachloride-induced hepatic fibrosis in mice
Background: Cornus wilsoniana Wanger is a widely distributed woody oil plant in south China; oil extracted from its fruits has been the main source of edible oil for local residents for hundreds of years. Previous studies have demonstrated that Cornus wilsoniana oil (CWO) has hypolipidemic activity...
| Main Authors: | , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Swedish Nutrition Foundation
2020-05-01
|
| Series: | Food & Nutrition Research |
| Subjects: | |
| Online Access: | https://foodandnutritionresearch.net/index.php/fnr/article/view/4205/10849 |
| _version_ | 1811292937157345280 |
|---|---|
| author | Qiang Liu Qiang Liu Xiaohua Lei Zhenyu Cao Ju Zhang Tao Kuang Guoxing Liu Yu Fang Ke Qian Jie Fu Huihui Du Likun Yan Zhihong Xiao Changzhu Li Xundi Xu |
| author_facet | Qiang Liu Qiang Liu Xiaohua Lei Zhenyu Cao Ju Zhang Tao Kuang Guoxing Liu Yu Fang Ke Qian Jie Fu Huihui Du Likun Yan Zhihong Xiao Changzhu Li Xundi Xu |
| author_sort | Qiang Liu |
| collection | DOAJ |
| description | Background: Cornus wilsoniana Wanger is a widely distributed woody oil plant in south China; oil extracted from its fruits has been the main source of edible oil for local residents for hundreds of years. Previous studies have demonstrated that Cornus wilsoniana oil (CWO) has hypolipidemic activity in rats. However, the hepatoprotective effects of CWO and their underlying mechanisms are not clear. Objective: The purpose of this study was to explore the protective effects and mechanisms of the CWO against carbon tetrachloride (CCl4)-induced hepatic fibrosis in mice. Methods: Hepatic fibrosis mouse model was induced by intraperitoneal injection with 1 mL/kg CCl4 (mixed 1:4 in olive oil) twice a week for 6 weeks. In the meantime, the mice were orally administrated with CWO (0.5, 2 mL/kg) once daily for 6 weeks. Serological changes as well as oxidative stress, inflammatory, and histological alteration in the liver were determined. Results: The results showed that CWO significantly attenuated CCl4-induced serological changes in mice, as assessed by serum markers, including alanine aminotransferase (ALT), aspartate aminotransferase (AST), procollagen III, collagen type IV, hyaluronic acid, and laminin. At the same time, CWO significantly improved CCl4-induced liver histological changes, as detected by hematoxylin and eosin (H&E), Sirius red, and Masson’s trichrome staining. In addition, treatment with CWO reduced oxidative stress and inflammation in the liver. Furthermore, CWO also reduced the expression of extracellular matrix (ECM) in liver induced by CCl4, and TGF-β1/Smad3 signaling may be involved in the process. Conclusions: CWO ameliorates CCl4-induced hepatic fibrosis by attenuating hepatic oxidative stress, reducing hepatic inflammation and inhibiting TGF-β1/Smad3 signaling pathway in liver. CWO may be a potentially beneficial edible oil for the adjuvant treatment of hepatic fibrosis. |
| first_indexed | 2024-04-13T04:52:59Z |
| format | Article |
| id | doaj.art-f1e1dfaa8d3a42dd8d82ee9717ea7c8e |
| institution | Directory Open Access Journal |
| issn | 1654-661X |
| language | English |
| last_indexed | 2024-04-13T04:52:59Z |
| publishDate | 2020-05-01 |
| publisher | Swedish Nutrition Foundation |
| record_format | Article |
| series | Food & Nutrition Research |
| spelling | doaj.art-f1e1dfaa8d3a42dd8d82ee9717ea7c8e2022-12-22T03:01:36ZengSwedish Nutrition FoundationFood & Nutrition Research1654-661X2020-05-0164011010.29219/fnr.v64.42054205Protective effect of oil from Cornus wilsoniana fruits against carbon tetrachloride-induced hepatic fibrosis in miceQiang Liu0Qiang Liu1Xiaohua Lei2Zhenyu Cao3Ju Zhang4Tao Kuang5Guoxing Liu6Yu Fang7Ke Qian8Jie Fu9Huihui Du10Likun Yan11Zhihong Xiao12Changzhu Li13Xundi Xu14Hunan Provincial Key Laboratory of Hepatobiliary Disease Research & Division of Hepato-Biliary-Pancreatic Surgery, Department of Surgery, The Second Xiangya Hospital, Central South University, Changsha, ChinaCollege of Life Sciences and Technology, Central South University of Forestry and Technology, Changsha, ChinaHunan Provincial Key Laboratory of Hepatobiliary Disease Research & Division of Hepato-Biliary-Pancreatic Surgery, Department of Surgery, The Second Xiangya Hospital, Central South University, Changsha, ChinaHunan Provincial Key Laboratory of Hepatobiliary Disease Research & Division of Hepato-Biliary-Pancreatic Surgery, Department of Surgery, The Second Xiangya Hospital, Central South University, Changsha, ChinaHunan Provincial Key Laboratory of Hepatobiliary Disease Research & Division of Hepato-Biliary-Pancreatic Surgery, Department of Surgery, The Second Xiangya Hospital, Central South University, Changsha, ChinaHunan Provincial Key Laboratory of Hepatobiliary Disease Research & Division of Hepato-Biliary-Pancreatic Surgery, Department of Surgery, The Second Xiangya Hospital, Central South University, Changsha, ChinaHunan Provincial Key Laboratory of Hepatobiliary Disease Research & Division of Hepato-Biliary-Pancreatic Surgery, Department of Surgery, The Second Xiangya Hospital, Central South University, Changsha, ChinaHunan Provincial Key Laboratory of Hepatobiliary Disease Research & Division of Hepato-Biliary-Pancreatic Surgery, Department of Surgery, The Second Xiangya Hospital, Central South University, Changsha, ChinaHunan Provincial Key Laboratory of Hepatobiliary Disease Research & Division of Hepato-Biliary-Pancreatic Surgery, Department of Surgery, The Second Xiangya Hospital, Central South University, Changsha, ChinaHunan Provincial Key Laboratory of Hepatobiliary Disease Research & Division of Hepato-Biliary-Pancreatic Surgery, Department of Surgery, The Second Xiangya Hospital, Central South University, Changsha, ChinaHunan Provincial Key Laboratory of Hepatobiliary Disease Research & Division of Hepato-Biliary-Pancreatic Surgery, Department of Surgery, The Second Xiangya Hospital, Central South University, Changsha, ChinaHunan Provincial Key Laboratory of Hepatobiliary Disease Research & Division of Hepato-Biliary-Pancreatic Surgery, Department of Surgery, The Second Xiangya Hospital, Central South University, Changsha, ChinaHunan Provincial key Laboratory of Oils & Fats Molecular Structure and Function, Hunan Academy of Forestry, Changsha, ChinaHunan Provincial key Laboratory of Oils & Fats Molecular Structure and Function, Hunan Academy of Forestry, Changsha, ChinaHunan Provincial Key Laboratory of Hepatobiliary Disease Research & Division of Hepato-Biliary-Pancreatic Surgery, Department of Surgery, The Second Xiangya Hospital, Central South University, Changsha, ChinaBackground: Cornus wilsoniana Wanger is a widely distributed woody oil plant in south China; oil extracted from its fruits has been the main source of edible oil for local residents for hundreds of years. Previous studies have demonstrated that Cornus wilsoniana oil (CWO) has hypolipidemic activity in rats. However, the hepatoprotective effects of CWO and their underlying mechanisms are not clear. Objective: The purpose of this study was to explore the protective effects and mechanisms of the CWO against carbon tetrachloride (CCl4)-induced hepatic fibrosis in mice. Methods: Hepatic fibrosis mouse model was induced by intraperitoneal injection with 1 mL/kg CCl4 (mixed 1:4 in olive oil) twice a week for 6 weeks. In the meantime, the mice were orally administrated with CWO (0.5, 2 mL/kg) once daily for 6 weeks. Serological changes as well as oxidative stress, inflammatory, and histological alteration in the liver were determined. Results: The results showed that CWO significantly attenuated CCl4-induced serological changes in mice, as assessed by serum markers, including alanine aminotransferase (ALT), aspartate aminotransferase (AST), procollagen III, collagen type IV, hyaluronic acid, and laminin. At the same time, CWO significantly improved CCl4-induced liver histological changes, as detected by hematoxylin and eosin (H&E), Sirius red, and Masson’s trichrome staining. In addition, treatment with CWO reduced oxidative stress and inflammation in the liver. Furthermore, CWO also reduced the expression of extracellular matrix (ECM) in liver induced by CCl4, and TGF-β1/Smad3 signaling may be involved in the process. Conclusions: CWO ameliorates CCl4-induced hepatic fibrosis by attenuating hepatic oxidative stress, reducing hepatic inflammation and inhibiting TGF-β1/Smad3 signaling pathway in liver. CWO may be a potentially beneficial edible oil for the adjuvant treatment of hepatic fibrosis.https://foodandnutritionresearch.net/index.php/fnr/article/view/4205/10849cornus wilsoniana oilhepatic fibrosisoxidative stressinflammationtgf-β1 |
| spellingShingle | Qiang Liu Qiang Liu Xiaohua Lei Zhenyu Cao Ju Zhang Tao Kuang Guoxing Liu Yu Fang Ke Qian Jie Fu Huihui Du Likun Yan Zhihong Xiao Changzhu Li Xundi Xu Protective effect of oil from Cornus wilsoniana fruits against carbon tetrachloride-induced hepatic fibrosis in mice Food & Nutrition Research cornus wilsoniana oil hepatic fibrosis oxidative stress inflammation tgf-β1 |
| title | Protective effect of oil from Cornus wilsoniana fruits against carbon tetrachloride-induced hepatic fibrosis in mice |
| title_full | Protective effect of oil from Cornus wilsoniana fruits against carbon tetrachloride-induced hepatic fibrosis in mice |
| title_fullStr | Protective effect of oil from Cornus wilsoniana fruits against carbon tetrachloride-induced hepatic fibrosis in mice |
| title_full_unstemmed | Protective effect of oil from Cornus wilsoniana fruits against carbon tetrachloride-induced hepatic fibrosis in mice |
| title_short | Protective effect of oil from Cornus wilsoniana fruits against carbon tetrachloride-induced hepatic fibrosis in mice |
| title_sort | protective effect of oil from cornus wilsoniana fruits against carbon tetrachloride induced hepatic fibrosis in mice |
| topic | cornus wilsoniana oil hepatic fibrosis oxidative stress inflammation tgf-β1 |
| url | https://foodandnutritionresearch.net/index.php/fnr/article/view/4205/10849 |
| work_keys_str_mv | AT qiangliu protectiveeffectofoilfromcornuswilsonianafruitsagainstcarbontetrachlorideinducedhepaticfibrosisinmice AT qiangliu protectiveeffectofoilfromcornuswilsonianafruitsagainstcarbontetrachlorideinducedhepaticfibrosisinmice AT xiaohualei protectiveeffectofoilfromcornuswilsonianafruitsagainstcarbontetrachlorideinducedhepaticfibrosisinmice AT zhenyucao protectiveeffectofoilfromcornuswilsonianafruitsagainstcarbontetrachlorideinducedhepaticfibrosisinmice AT juzhang protectiveeffectofoilfromcornuswilsonianafruitsagainstcarbontetrachlorideinducedhepaticfibrosisinmice AT taokuang protectiveeffectofoilfromcornuswilsonianafruitsagainstcarbontetrachlorideinducedhepaticfibrosisinmice AT guoxingliu protectiveeffectofoilfromcornuswilsonianafruitsagainstcarbontetrachlorideinducedhepaticfibrosisinmice AT yufang protectiveeffectofoilfromcornuswilsonianafruitsagainstcarbontetrachlorideinducedhepaticfibrosisinmice AT keqian protectiveeffectofoilfromcornuswilsonianafruitsagainstcarbontetrachlorideinducedhepaticfibrosisinmice AT jiefu protectiveeffectofoilfromcornuswilsonianafruitsagainstcarbontetrachlorideinducedhepaticfibrosisinmice AT huihuidu protectiveeffectofoilfromcornuswilsonianafruitsagainstcarbontetrachlorideinducedhepaticfibrosisinmice AT likunyan protectiveeffectofoilfromcornuswilsonianafruitsagainstcarbontetrachlorideinducedhepaticfibrosisinmice AT zhihongxiao protectiveeffectofoilfromcornuswilsonianafruitsagainstcarbontetrachlorideinducedhepaticfibrosisinmice AT changzhuli protectiveeffectofoilfromcornuswilsonianafruitsagainstcarbontetrachlorideinducedhepaticfibrosisinmice AT xundixu protectiveeffectofoilfromcornuswilsonianafruitsagainstcarbontetrachlorideinducedhepaticfibrosisinmice |