Characterization and Cytotoxic Activity of Microwave-Assisted Extracted Crude Fucoidans from Different Brown Seaweeds
Microwave-assisted extraction (MAE) is recognized as a green method for extraction of natural products. The current research aimed to explore the MAE for fucoidans extraction from different brown seaweeds, including <i>Fucus vesiculosus</i>, <i>F. spiralis</i>, and <i>L...
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| Format: | Article |
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MDPI AG
2023-01-01
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| Series: | Marine Drugs |
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| Online Access: | https://www.mdpi.com/1660-3397/21/1/48 |
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| author | Ahmed Zayed Doris Finkelmeier Thomas Hahn Lisa Rebers Anusriha Shanmugam Anke Burger-Kentischer Roland Ulber |
| author_facet | Ahmed Zayed Doris Finkelmeier Thomas Hahn Lisa Rebers Anusriha Shanmugam Anke Burger-Kentischer Roland Ulber |
| author_sort | Ahmed Zayed |
| collection | DOAJ |
| description | Microwave-assisted extraction (MAE) is recognized as a green method for extraction of natural products. The current research aimed to explore the MAE for fucoidans extraction from different brown seaweeds, including <i>Fucus vesiculosus</i>, <i>F. spiralis</i>, and <i>Laminaria saccharina</i>. Following several solvent-extraction pre-treatment steps and MAE optimization, the algal biomasses were extracted in a ratio of 1:25 in 0.1 M HCl containing 2 M CaCl<sub>2</sub> for 1.0 min. The results showed that <i>L. saccharina</i>’s extract was different from the others, regarding the highest sugar content reached 0.47 mg glucose equivalent/mg extract being confirmed by monosaccharide composition analysis and the lowest fucoidan content and sulfation degree at 0.09 mg/mg extract and 0.13, respectively. Moreover, these findings were confirmed by tentative structural elucidation based on Fourier-transform infrared spectrometry which also showed a different spectrum. However, the MAE enhanced melanoidins formation in products, which was confirmed by the intense band at 1420 cm<sup>−1</sup>. Interestingly, the results of monomeric composition showed that fucoidan extract by MAE from <i>F. vesiculosus</i> belonged to sulfated galactofucans which are known for their potential bioactivities. Furthermore, the cytotoxic activity of the four fucoidans in concentrations ranging from 4.9 µg/mL to 2500 µg/mL was investigated and correlated with the chemical characterization showing that <i>F. vesiculosus</i>_MAE fucoidan was the most potent and safest. The current research revealed the chemical heterogeneity of fucoidans regarding taxonomical class and used greener extraction method of fucoidans toward the achievement of the UN sustainability goals. |
| first_indexed | 2024-03-09T11:52:59Z |
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| institution | Directory Open Access Journal |
| issn | 1660-3397 |
| language | English |
| last_indexed | 2024-03-09T11:52:59Z |
| publishDate | 2023-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Marine Drugs |
| spelling | doaj.art-f1e92ee430404b8d92fcaebae6bb35012023-11-30T23:13:48ZengMDPI AGMarine Drugs1660-33972023-01-012114810.3390/md21010048Characterization and Cytotoxic Activity of Microwave-Assisted Extracted Crude Fucoidans from Different Brown SeaweedsAhmed Zayed0Doris Finkelmeier1Thomas Hahn2Lisa Rebers3Anusriha Shanmugam4Anke Burger-Kentischer5Roland Ulber6Institute of Bioprocess Engineering, Rheinland-Pfälzische Technische Universität Kaiserslautern-Landau, Gottlieb-Daimler-Straße 49, 67663 Kaiserslautern, GermanyInnovation Field Cell and Tissue Technologies, Fraunhofer Institute for Interfacial Engineering and Biotechnology IGB, Nobelstraße 12, 70569 Stuttgart, GermanyInnovation Field Industrial Biotechnology, Fraunhofer Institute for Interfacial Engineering and Biotechnology IGB, Nobelstraße 12, 70569 Stuttgart, GermanyInnovation Field Cell and Tissue Technologies, Fraunhofer Institute for Interfacial Engineering and Biotechnology IGB, Nobelstraße 12, 70569 Stuttgart, GermanyBiology Department, Rheinland-Pfälzische Technische Universität Kaiserslautern-Landau, Erwin-Schrödinger-Straße 13, 67663 Kaiserslautern, GermanyInnovation Field Cell and Tissue Technologies, Fraunhofer Institute for Interfacial Engineering and Biotechnology IGB, Nobelstraße 12, 70569 Stuttgart, GermanyInstitute of Bioprocess Engineering, Rheinland-Pfälzische Technische Universität Kaiserslautern-Landau, Gottlieb-Daimler-Straße 49, 67663 Kaiserslautern, GermanyMicrowave-assisted extraction (MAE) is recognized as a green method for extraction of natural products. The current research aimed to explore the MAE for fucoidans extraction from different brown seaweeds, including <i>Fucus vesiculosus</i>, <i>F. spiralis</i>, and <i>Laminaria saccharina</i>. Following several solvent-extraction pre-treatment steps and MAE optimization, the algal biomasses were extracted in a ratio of 1:25 in 0.1 M HCl containing 2 M CaCl<sub>2</sub> for 1.0 min. The results showed that <i>L. saccharina</i>’s extract was different from the others, regarding the highest sugar content reached 0.47 mg glucose equivalent/mg extract being confirmed by monosaccharide composition analysis and the lowest fucoidan content and sulfation degree at 0.09 mg/mg extract and 0.13, respectively. Moreover, these findings were confirmed by tentative structural elucidation based on Fourier-transform infrared spectrometry which also showed a different spectrum. However, the MAE enhanced melanoidins formation in products, which was confirmed by the intense band at 1420 cm<sup>−1</sup>. Interestingly, the results of monomeric composition showed that fucoidan extract by MAE from <i>F. vesiculosus</i> belonged to sulfated galactofucans which are known for their potential bioactivities. Furthermore, the cytotoxic activity of the four fucoidans in concentrations ranging from 4.9 µg/mL to 2500 µg/mL was investigated and correlated with the chemical characterization showing that <i>F. vesiculosus</i>_MAE fucoidan was the most potent and safest. The current research revealed the chemical heterogeneity of fucoidans regarding taxonomical class and used greener extraction method of fucoidans toward the achievement of the UN sustainability goals.https://www.mdpi.com/1660-3397/21/1/48anti-tumorfucoidansextractionmicrowave-assistedbioactivitybrown algae |
| spellingShingle | Ahmed Zayed Doris Finkelmeier Thomas Hahn Lisa Rebers Anusriha Shanmugam Anke Burger-Kentischer Roland Ulber Characterization and Cytotoxic Activity of Microwave-Assisted Extracted Crude Fucoidans from Different Brown Seaweeds Marine Drugs anti-tumor fucoidans extraction microwave-assisted bioactivity brown algae |
| title | Characterization and Cytotoxic Activity of Microwave-Assisted Extracted Crude Fucoidans from Different Brown Seaweeds |
| title_full | Characterization and Cytotoxic Activity of Microwave-Assisted Extracted Crude Fucoidans from Different Brown Seaweeds |
| title_fullStr | Characterization and Cytotoxic Activity of Microwave-Assisted Extracted Crude Fucoidans from Different Brown Seaweeds |
| title_full_unstemmed | Characterization and Cytotoxic Activity of Microwave-Assisted Extracted Crude Fucoidans from Different Brown Seaweeds |
| title_short | Characterization and Cytotoxic Activity of Microwave-Assisted Extracted Crude Fucoidans from Different Brown Seaweeds |
| title_sort | characterization and cytotoxic activity of microwave assisted extracted crude fucoidans from different brown seaweeds |
| topic | anti-tumor fucoidans extraction microwave-assisted bioactivity brown algae |
| url | https://www.mdpi.com/1660-3397/21/1/48 |
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