Injectable Hydrogel Membrane for Guided Bone Regeneration
In recent years, multicomponent hydrogels such as interpenetrating polymer networks (IPNs) have emerged as innovative biomaterials due to the synergistic combination of the properties of each network. We hypothesized that an innovative non-animal IPN hydrogel combining self-setting silanized hydroxy...
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-01-01
|
| Series: | Bioengineering |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2306-5354/10/1/94 |
| _version_ | 1827628518021791744 |
|---|---|
| author | Pauline Marie Chichiricco Pietro Matricardi Bruno Colaço Pedro Gomes Christine Jérôme Julie Lesoeur Joëlle Veziers Gildas Réthoré Pierre Weiss Xavier Struillou Catherine Le Visage |
| author_facet | Pauline Marie Chichiricco Pietro Matricardi Bruno Colaço Pedro Gomes Christine Jérôme Julie Lesoeur Joëlle Veziers Gildas Réthoré Pierre Weiss Xavier Struillou Catherine Le Visage |
| author_sort | Pauline Marie Chichiricco |
| collection | DOAJ |
| description | In recent years, multicomponent hydrogels such as interpenetrating polymer networks (IPNs) have emerged as innovative biomaterials due to the synergistic combination of the properties of each network. We hypothesized that an innovative non-animal IPN hydrogel combining self-setting silanized hydroxypropyl methylcellulose (Si-HPMC) with photochemically cross-linkable dextran methacrylate (DexMA) could be a valid alternative to porcine collagen membranes in guided bone regeneration. Calvaria critical-size defects in rabbits were filled with synthetic biphasic calcium phosphate granules in conjunction with Si-HPMC; DexMA; or Si-HPMC/DexMA experimental membranes; and in a control group with a porcine collagen membrane. The synergistic effect obtained by interpenetration of the two polymer networks improved the physicochemical properties, and the gel point under visible light was reached instantaneously. Neutral red staining of murine L929 fibroblasts confirmed the cytocompatibility of the IPN. At 8 weeks, the photo-crosslinked membranes induced a similar degree of mineral deposition in the calvaria defects compared to the positive control, with 30.5 ± 5.2% for the IPN and 34.3 ± 8.2% for the collagen membrane. The barrier effect appeared to be similar in the IPN test group compared with the collagen membrane. In conclusion, this novel, easy-to-handle and apply, photochemically cross-linkable IPN hydrogel is an excellent non-animal alternative to porcine collagen membrane in guided bone regeneration procedures. |
| first_indexed | 2024-03-09T13:33:40Z |
| format | Article |
| id | doaj.art-f1eb8fe4161e4e09b814429401f9f519 |
| institution | Directory Open Access Journal |
| issn | 2306-5354 |
| language | English |
| last_indexed | 2024-03-09T13:33:40Z |
| publishDate | 2023-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Bioengineering |
| spelling | doaj.art-f1eb8fe4161e4e09b814429401f9f5192023-11-30T21:15:33ZengMDPI AGBioengineering2306-53542023-01-011019410.3390/bioengineering10010094Injectable Hydrogel Membrane for Guided Bone RegenerationPauline Marie Chichiricco0Pietro Matricardi1Bruno Colaço2Pedro Gomes3Christine Jérôme4Julie Lesoeur5Joëlle Veziers6Gildas Réthoré7Pierre Weiss8Xavier Struillou9Catherine Le Visage10CESAM Research Unit, Center for Education and Research on Macromolecules (CERM), University of Liège, B-4000 Liège, BelgiumDepartment of Drug Chemistry and Technologies, Sapienza University, 000185 Rome, ItalyDepartment of Animal Sciences, Animal and Veterinary Research Centre, University of Trás-os-Montes and Alto Douro, 500-801 Vila Real, PortugalFMDUP, Laboratory for Bone Metabolism and Regeneration, Faculty of Dental Medicine, University of Porto, 500-801 Porto, PortugalCESAM Research Unit, Center for Education and Research on Macromolecules (CERM), University of Liège, B-4000 Liège, BelgiumNantes Université, Oniris, Univ Angers, INSERM, Regenerative Medicine and Skeleton, RMeS, UMR 1229, F-44000 Nantes, FranceNantes Université, Oniris, Univ Angers, INSERM, Regenerative Medicine and Skeleton, RMeS, UMR 1229, F-44000 Nantes, FranceNantes Université, Oniris, Univ Angers, INSERM, Regenerative Medicine and Skeleton, RMeS, UMR 1229, F-44000 Nantes, FranceNantes Université, Oniris, Univ Angers, INSERM, Regenerative Medicine and Skeleton, RMeS, UMR 1229, F-44000 Nantes, FranceNantes Université, Oniris, Univ Angers, INSERM, Regenerative Medicine and Skeleton, RMeS, UMR 1229, F-44000 Nantes, FranceNantes Université, Oniris, Univ Angers, INSERM, Regenerative Medicine and Skeleton, RMeS, UMR 1229, F-44000 Nantes, FranceIn recent years, multicomponent hydrogels such as interpenetrating polymer networks (IPNs) have emerged as innovative biomaterials due to the synergistic combination of the properties of each network. We hypothesized that an innovative non-animal IPN hydrogel combining self-setting silanized hydroxypropyl methylcellulose (Si-HPMC) with photochemically cross-linkable dextran methacrylate (DexMA) could be a valid alternative to porcine collagen membranes in guided bone regeneration. Calvaria critical-size defects in rabbits were filled with synthetic biphasic calcium phosphate granules in conjunction with Si-HPMC; DexMA; or Si-HPMC/DexMA experimental membranes; and in a control group with a porcine collagen membrane. The synergistic effect obtained by interpenetration of the two polymer networks improved the physicochemical properties, and the gel point under visible light was reached instantaneously. Neutral red staining of murine L929 fibroblasts confirmed the cytocompatibility of the IPN. At 8 weeks, the photo-crosslinked membranes induced a similar degree of mineral deposition in the calvaria defects compared to the positive control, with 30.5 ± 5.2% for the IPN and 34.3 ± 8.2% for the collagen membrane. The barrier effect appeared to be similar in the IPN test group compared with the collagen membrane. In conclusion, this novel, easy-to-handle and apply, photochemically cross-linkable IPN hydrogel is an excellent non-animal alternative to porcine collagen membrane in guided bone regeneration procedures.https://www.mdpi.com/2306-5354/10/1/94photo-crosslinkingvisible light photopolymerizationsilanized hydroxypropyl methylcellulosedextran methacrylateriboflavincalvaria bone regeneration |
| spellingShingle | Pauline Marie Chichiricco Pietro Matricardi Bruno Colaço Pedro Gomes Christine Jérôme Julie Lesoeur Joëlle Veziers Gildas Réthoré Pierre Weiss Xavier Struillou Catherine Le Visage Injectable Hydrogel Membrane for Guided Bone Regeneration Bioengineering photo-crosslinking visible light photopolymerization silanized hydroxypropyl methylcellulose dextran methacrylate riboflavin calvaria bone regeneration |
| title | Injectable Hydrogel Membrane for Guided Bone Regeneration |
| title_full | Injectable Hydrogel Membrane for Guided Bone Regeneration |
| title_fullStr | Injectable Hydrogel Membrane for Guided Bone Regeneration |
| title_full_unstemmed | Injectable Hydrogel Membrane for Guided Bone Regeneration |
| title_short | Injectable Hydrogel Membrane for Guided Bone Regeneration |
| title_sort | injectable hydrogel membrane for guided bone regeneration |
| topic | photo-crosslinking visible light photopolymerization silanized hydroxypropyl methylcellulose dextran methacrylate riboflavin calvaria bone regeneration |
| url | https://www.mdpi.com/2306-5354/10/1/94 |
| work_keys_str_mv | AT paulinemariechichiricco injectablehydrogelmembraneforguidedboneregeneration AT pietromatricardi injectablehydrogelmembraneforguidedboneregeneration AT brunocolaco injectablehydrogelmembraneforguidedboneregeneration AT pedrogomes injectablehydrogelmembraneforguidedboneregeneration AT christinejerome injectablehydrogelmembraneforguidedboneregeneration AT julielesoeur injectablehydrogelmembraneforguidedboneregeneration AT joelleveziers injectablehydrogelmembraneforguidedboneregeneration AT gildasrethore injectablehydrogelmembraneforguidedboneregeneration AT pierreweiss injectablehydrogelmembraneforguidedboneregeneration AT xavierstruillou injectablehydrogelmembraneforguidedboneregeneration AT catherinelevisage injectablehydrogelmembraneforguidedboneregeneration |