Serological, molecular, and microscopic detection of Leishmania in cats (Felis catus) in Belo Horizonte, Minas Gerais State, Brazil
Abstract The role of cats in the epidemiological cycle of leishmaniasis remains unclear. To better understand the occurrence of leishmaniasis in cats, we studied the frequency of Leishmania in serum samples of 100 cats living in an endemic region for canine and human leishmaniasis by serological, p...
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| Format: | Article |
| Language: | English |
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Colégio Brasileiro de Parasitologia Veterinaria
2018-08-01
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| Series: | Revista Brasileira de Parasitologia Veterinária |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612018005010106&lng=en&tlng=en |
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| author | Fernanda Morcatti Coura Stephanie Karoline Pereira Passos Marina de Oliveira França Pelegrino Fabiola de Oliveira Paes Leme Gustavo Fontes Paz Célia Maria Ferreira Gontijo Adriane Pimenta da Costa-Val |
| author_facet | Fernanda Morcatti Coura Stephanie Karoline Pereira Passos Marina de Oliveira França Pelegrino Fabiola de Oliveira Paes Leme Gustavo Fontes Paz Célia Maria Ferreira Gontijo Adriane Pimenta da Costa-Val |
| author_sort | Fernanda Morcatti Coura |
| collection | DOAJ |
| description | Abstract The role of cats in the epidemiological cycle of leishmaniasis remains unclear. To better understand the occurrence of leishmaniasis in cats, we studied the frequency of Leishmania in serum samples of 100 cats living in an endemic region for canine and human leishmaniasis by serological, parasitological, and molecular methods. Of the 100 cats, 54 were seropositive for Leishmania antibodies by immunofluorescence antibody test. None of the bone marrow aspirates collected from these cats tested positive for the parasite in culture or upon polymerase chain reaction (PCR) analysis. Biopsy samples of the ears also tested negative for Leishmania upon PCR analysis. These findings may indicate that the region is endemic for canine leishmaniasis and cats are infected by Leishmania; or that cross-reaction with antibodies against other parasites increases the frequency of seropositivity; or that cats respond to Leishmania infection by producing antibodies when few or no parasites are present in bone marrow and tissue samples. Overall, our results suggest that cats can be infected by Leishmania ; however, we failed to demonstrate feline parasitosis. These findings highlight the need to study leishmaniasis in cats, since sandflies feed on cats, these animals may act as a reservoir for the parasite. |
| first_indexed | 2024-12-11T22:19:24Z |
| format | Article |
| id | doaj.art-f1ed7786a2364be2a45fa59dc342bcdd |
| institution | Directory Open Access Journal |
| issn | 1984-2961 |
| language | English |
| last_indexed | 2024-12-11T22:19:24Z |
| publishDate | 2018-08-01 |
| publisher | Colégio Brasileiro de Parasitologia Veterinaria |
| record_format | Article |
| series | Revista Brasileira de Parasitologia Veterinária |
| spelling | doaj.art-f1ed7786a2364be2a45fa59dc342bcdd2022-12-22T00:48:30ZengColégio Brasileiro de Parasitologia VeterinariaRevista Brasileira de Parasitologia Veterinária1984-29612018-08-01010.1590/s1984-296120180052S1984-29612018005010106Serological, molecular, and microscopic detection of Leishmania in cats (Felis catus) in Belo Horizonte, Minas Gerais State, BrazilFernanda Morcatti CouraStephanie Karoline Pereira PassosMarina de Oliveira França PelegrinoFabiola de Oliveira Paes LemeGustavo Fontes PazCélia Maria Ferreira GontijoAdriane Pimenta da Costa-ValAbstract The role of cats in the epidemiological cycle of leishmaniasis remains unclear. To better understand the occurrence of leishmaniasis in cats, we studied the frequency of Leishmania in serum samples of 100 cats living in an endemic region for canine and human leishmaniasis by serological, parasitological, and molecular methods. Of the 100 cats, 54 were seropositive for Leishmania antibodies by immunofluorescence antibody test. None of the bone marrow aspirates collected from these cats tested positive for the parasite in culture or upon polymerase chain reaction (PCR) analysis. Biopsy samples of the ears also tested negative for Leishmania upon PCR analysis. These findings may indicate that the region is endemic for canine leishmaniasis and cats are infected by Leishmania; or that cross-reaction with antibodies against other parasites increases the frequency of seropositivity; or that cats respond to Leishmania infection by producing antibodies when few or no parasites are present in bone marrow and tissue samples. Overall, our results suggest that cats can be infected by Leishmania ; however, we failed to demonstrate feline parasitosis. These findings highlight the need to study leishmaniasis in cats, since sandflies feed on cats, these animals may act as a reservoir for the parasite.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612018005010106&lng=en&tlng=enDiagnósticoleishmaniose felinareservatório |
| spellingShingle | Fernanda Morcatti Coura Stephanie Karoline Pereira Passos Marina de Oliveira França Pelegrino Fabiola de Oliveira Paes Leme Gustavo Fontes Paz Célia Maria Ferreira Gontijo Adriane Pimenta da Costa-Val Serological, molecular, and microscopic detection of Leishmania in cats (Felis catus) in Belo Horizonte, Minas Gerais State, Brazil Revista Brasileira de Parasitologia Veterinária Diagnóstico leishmaniose felina reservatório |
| title | Serological, molecular, and microscopic detection of Leishmania in cats (Felis catus) in Belo Horizonte, Minas Gerais State, Brazil |
| title_full | Serological, molecular, and microscopic detection of Leishmania in cats (Felis catus) in Belo Horizonte, Minas Gerais State, Brazil |
| title_fullStr | Serological, molecular, and microscopic detection of Leishmania in cats (Felis catus) in Belo Horizonte, Minas Gerais State, Brazil |
| title_full_unstemmed | Serological, molecular, and microscopic detection of Leishmania in cats (Felis catus) in Belo Horizonte, Minas Gerais State, Brazil |
| title_short | Serological, molecular, and microscopic detection of Leishmania in cats (Felis catus) in Belo Horizonte, Minas Gerais State, Brazil |
| title_sort | serological molecular and microscopic detection of leishmania in cats felis catus in belo horizonte minas gerais state brazil |
| topic | Diagnóstico leishmaniose felina reservatório |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612018005010106&lng=en&tlng=en |
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