Peripheral blood CD4+ T cell populations by CD25 and Foxp3 expression as a potential biomarker: reflecting inflammatory activity in chronic obstructive pulmonary disease
Zhao-Ji Meng,* Jiang-Hua Wu,* Mei Zhou, Sheng-Wen Sun, Shuai-Ying Miao, Hong-Li Han, Long Chen, Xian-Zhi XiongDepartment of Respiratory and Critical Care Medicine, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, People’s Republic of Chin...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Dove Medical Press
2019-07-01
|
| Series: | International Journal of COPD |
| Subjects: | |
| Online Access: | https://www.dovepress.com/peripheral-blood-cd4-t-cell-populations-by-cd25-and-foxp3-expression-a-peer-reviewed-article-COPD |
| _version_ | 1819117453788053504 |
|---|---|
| author | Meng ZJ Wu JH Zhou M Sun SW Miao SY Han HL Chen L Xiong XZ |
| author_facet | Meng ZJ Wu JH Zhou M Sun SW Miao SY Han HL Chen L Xiong XZ |
| author_sort | Meng ZJ |
| collection | DOAJ |
| description | Zhao-Ji Meng,* Jiang-Hua Wu,* Mei Zhou, Sheng-Wen Sun, Shuai-Ying Miao, Hong-Li Han, Long Chen, Xian-Zhi XiongDepartment of Respiratory and Critical Care Medicine, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, People’s Republic of China*These authors contributed equally to this workBackground: The temporally dynamic changes of CD25 and Foxp3 expression in CD4+ T cells are initiated by T cell receptor (TCR) signals strength or frequency. There is a deficiency of peripheral markers for assessing COPD activity, and the current study was conducted to explore whether peripheral CD4+ T cell populations based on CD25 and Foxp3 expression could serve as an indicator for COPD inflammatory activity.Methods: The distribution and phenotypic characteristics of CD4+CD25±Foxp3± T cells from peripheral blood in different populations were determined by flow cytometry. The model for the differentiation of CD4+ T cells populations by CD25 and Foxp3 expression was explored in vitro.Results: The frequencies of peripheral CD4+CD25+Foxp3− T cells and CD4+CD25+Foxp3+ T cells were increased in AECOPD patients, whereas the frequency of CD4+CD25−Foxp3+ T cells was increased in SCOPD patients without receiving systemic treatment. Phenotypic analysis revealed that CD4+CD25+Foxp3− T cells, CD4+CD25+Foxp3+ T cells and CD4+CD25−Foxp3+ T cells had received antigenic stimulation and resembled central memory or effector memory T cells. The differentiation of CD4+ T cells populations by CD25 and Foxp3 expression was dictated by TCR signals. The paired study indicated that the frequencies of CD4+CD25+Foxp3− T cells, CD4+CD25+Foxp3+ T cells and CD4+CD25− Foxp3+ T cells were decreased while the frequency of CD4+CD25−Foxp3− T cells were increased in the same patients from AECOPD to convalescence.Conclusions: Collectively, we propose that the dynamic changes of CD4+ T cell populations by CD25 and Foxp3 expression could function as potential biomarkers for reflecting inflammatory activity in COPD.Keywords: COPD, CD4+ T cell subsets, inflammation, peripheral biomarkers |
| first_indexed | 2024-12-22T05:33:14Z |
| format | Article |
| id | doaj.art-f1ed9b2e38b84c25b788923304dd730e |
| institution | Directory Open Access Journal |
| issn | 1178-2005 |
| language | English |
| last_indexed | 2024-12-22T05:33:14Z |
| publishDate | 2019-07-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | International Journal of COPD |
| spelling | doaj.art-f1ed9b2e38b84c25b788923304dd730e2022-12-21T18:37:24ZengDove Medical PressInternational Journal of COPD1178-20052019-07-01Volume 141669168047498Peripheral blood CD4+ T cell populations by CD25 and Foxp3 expression as a potential biomarker: reflecting inflammatory activity in chronic obstructive pulmonary diseaseMeng ZJWu JHZhou MSun SWMiao SYHan HLChen LXiong XZZhao-Ji Meng,* Jiang-Hua Wu,* Mei Zhou, Sheng-Wen Sun, Shuai-Ying Miao, Hong-Li Han, Long Chen, Xian-Zhi XiongDepartment of Respiratory and Critical Care Medicine, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, People’s Republic of China*These authors contributed equally to this workBackground: The temporally dynamic changes of CD25 and Foxp3 expression in CD4+ T cells are initiated by T cell receptor (TCR) signals strength or frequency. There is a deficiency of peripheral markers for assessing COPD activity, and the current study was conducted to explore whether peripheral CD4+ T cell populations based on CD25 and Foxp3 expression could serve as an indicator for COPD inflammatory activity.Methods: The distribution and phenotypic characteristics of CD4+CD25±Foxp3± T cells from peripheral blood in different populations were determined by flow cytometry. The model for the differentiation of CD4+ T cells populations by CD25 and Foxp3 expression was explored in vitro.Results: The frequencies of peripheral CD4+CD25+Foxp3− T cells and CD4+CD25+Foxp3+ T cells were increased in AECOPD patients, whereas the frequency of CD4+CD25−Foxp3+ T cells was increased in SCOPD patients without receiving systemic treatment. Phenotypic analysis revealed that CD4+CD25+Foxp3− T cells, CD4+CD25+Foxp3+ T cells and CD4+CD25−Foxp3+ T cells had received antigenic stimulation and resembled central memory or effector memory T cells. The differentiation of CD4+ T cells populations by CD25 and Foxp3 expression was dictated by TCR signals. The paired study indicated that the frequencies of CD4+CD25+Foxp3− T cells, CD4+CD25+Foxp3+ T cells and CD4+CD25− Foxp3+ T cells were decreased while the frequency of CD4+CD25−Foxp3− T cells were increased in the same patients from AECOPD to convalescence.Conclusions: Collectively, we propose that the dynamic changes of CD4+ T cell populations by CD25 and Foxp3 expression could function as potential biomarkers for reflecting inflammatory activity in COPD.Keywords: COPD, CD4+ T cell subsets, inflammation, peripheral biomarkershttps://www.dovepress.com/peripheral-blood-cd4-t-cell-populations-by-cd25-and-foxp3-expression-a-peer-reviewed-article-COPDCOPDCD4+ T cell subsetsinflammationperipheral biomarkers |
| spellingShingle | Meng ZJ Wu JH Zhou M Sun SW Miao SY Han HL Chen L Xiong XZ Peripheral blood CD4+ T cell populations by CD25 and Foxp3 expression as a potential biomarker: reflecting inflammatory activity in chronic obstructive pulmonary disease International Journal of COPD COPD CD4+ T cell subsets inflammation peripheral biomarkers |
| title | Peripheral blood CD4+ T cell populations by CD25 and Foxp3 expression as a potential biomarker: reflecting inflammatory activity in chronic obstructive pulmonary disease |
| title_full | Peripheral blood CD4+ T cell populations by CD25 and Foxp3 expression as a potential biomarker: reflecting inflammatory activity in chronic obstructive pulmonary disease |
| title_fullStr | Peripheral blood CD4+ T cell populations by CD25 and Foxp3 expression as a potential biomarker: reflecting inflammatory activity in chronic obstructive pulmonary disease |
| title_full_unstemmed | Peripheral blood CD4+ T cell populations by CD25 and Foxp3 expression as a potential biomarker: reflecting inflammatory activity in chronic obstructive pulmonary disease |
| title_short | Peripheral blood CD4+ T cell populations by CD25 and Foxp3 expression as a potential biomarker: reflecting inflammatory activity in chronic obstructive pulmonary disease |
| title_sort | peripheral blood cd4 t cell populations by cd25 and foxp3 expression as a potential biomarker reflecting inflammatory activity in chronic obstructive pulmonary disease |
| topic | COPD CD4+ T cell subsets inflammation peripheral biomarkers |
| url | https://www.dovepress.com/peripheral-blood-cd4-t-cell-populations-by-cd25-and-foxp3-expression-a-peer-reviewed-article-COPD |
| work_keys_str_mv | AT mengzj peripheralbloodcd4tcellpopulationsbycd25andfoxp3expressionasapotentialbiomarkerreflectinginflammatoryactivityinchronicobstructivepulmonarydisease AT wujh peripheralbloodcd4tcellpopulationsbycd25andfoxp3expressionasapotentialbiomarkerreflectinginflammatoryactivityinchronicobstructivepulmonarydisease AT zhoum peripheralbloodcd4tcellpopulationsbycd25andfoxp3expressionasapotentialbiomarkerreflectinginflammatoryactivityinchronicobstructivepulmonarydisease AT sunsw peripheralbloodcd4tcellpopulationsbycd25andfoxp3expressionasapotentialbiomarkerreflectinginflammatoryactivityinchronicobstructivepulmonarydisease AT miaosy peripheralbloodcd4tcellpopulationsbycd25andfoxp3expressionasapotentialbiomarkerreflectinginflammatoryactivityinchronicobstructivepulmonarydisease AT hanhl peripheralbloodcd4tcellpopulationsbycd25andfoxp3expressionasapotentialbiomarkerreflectinginflammatoryactivityinchronicobstructivepulmonarydisease AT chenl peripheralbloodcd4tcellpopulationsbycd25andfoxp3expressionasapotentialbiomarkerreflectinginflammatoryactivityinchronicobstructivepulmonarydisease AT xiongxz peripheralbloodcd4tcellpopulationsbycd25andfoxp3expressionasapotentialbiomarkerreflectinginflammatoryactivityinchronicobstructivepulmonarydisease |