Bioprospecting Through Cloning of Whole Natural Product Biosynthetic Gene Clusters
Since the discovery of penicillin, natural products and their derivatives have been a valuable resource for drug discovery. With recent development of genome mining approaches in the post-genome era, a great number of natural product biosynthetic gene clusters (BGCs) have been identified and these c...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2020-06-01
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| Series: | Frontiers in Bioengineering and Biotechnology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/article/10.3389/fbioe.2020.00526/full |
| _version_ | 1818136589706985472 |
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| author | Zhenquan Lin Jens Nielsen Jens Nielsen Jens Nielsen Jens Nielsen Zihe Liu |
| author_facet | Zhenquan Lin Jens Nielsen Jens Nielsen Jens Nielsen Jens Nielsen Zihe Liu |
| author_sort | Zhenquan Lin |
| collection | DOAJ |
| description | Since the discovery of penicillin, natural products and their derivatives have been a valuable resource for drug discovery. With recent development of genome mining approaches in the post-genome era, a great number of natural product biosynthetic gene clusters (BGCs) have been identified and these can potentially be exploited for the discovery of novel natural products that can find application as pharmaceuticals. Since many BGCs are silent or do not express in native hosts under laboratory conditions, heterologous expression of BGCs in genetically tractable hosts becomes an attractive route to activate these BGCs to discover the corresponding products. Here, we highlight recent achievements in cloning and discovery of natural product biosynthetic pathways via intact BGC capturing, and discuss the prospects of high-throughput and multiplexed cloning of rational-designed gene clusters in the future. |
| first_indexed | 2024-12-11T09:42:49Z |
| format | Article |
| id | doaj.art-f1ef40024bec4759a3a1ceed1274a1dd |
| institution | Directory Open Access Journal |
| issn | 2296-4185 |
| language | English |
| last_indexed | 2024-12-11T09:42:49Z |
| publishDate | 2020-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Bioengineering and Biotechnology |
| spelling | doaj.art-f1ef40024bec4759a3a1ceed1274a1dd2022-12-22T01:12:39ZengFrontiers Media S.A.Frontiers in Bioengineering and Biotechnology2296-41852020-06-01810.3389/fbioe.2020.00526545512Bioprospecting Through Cloning of Whole Natural Product Biosynthetic Gene ClustersZhenquan Lin0Jens Nielsen1Jens Nielsen2Jens Nielsen3Jens Nielsen4Zihe Liu5Beijing Advanced Innovation Center for Soft Matter Science and Engineering, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing, ChinaBeijing Advanced Innovation Center for Soft Matter Science and Engineering, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing, ChinaDepartment of Biology and Biological Engineering, Chalmers University of Technology, Gothenburg, SwedenNovo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Lyngby, DenmarkBioInnovation Institute, Copenhagen, DenmarkBeijing Advanced Innovation Center for Soft Matter Science and Engineering, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing, ChinaSince the discovery of penicillin, natural products and their derivatives have been a valuable resource for drug discovery. With recent development of genome mining approaches in the post-genome era, a great number of natural product biosynthetic gene clusters (BGCs) have been identified and these can potentially be exploited for the discovery of novel natural products that can find application as pharmaceuticals. Since many BGCs are silent or do not express in native hosts under laboratory conditions, heterologous expression of BGCs in genetically tractable hosts becomes an attractive route to activate these BGCs to discover the corresponding products. Here, we highlight recent achievements in cloning and discovery of natural product biosynthetic pathways via intact BGC capturing, and discuss the prospects of high-throughput and multiplexed cloning of rational-designed gene clusters in the future.https://www.frontiersin.org/article/10.3389/fbioe.2020.00526/fullnatural productbiosynthetic gene clusterheterologous expressionsequence-independent cloningdirect cloning |
| spellingShingle | Zhenquan Lin Jens Nielsen Jens Nielsen Jens Nielsen Jens Nielsen Zihe Liu Bioprospecting Through Cloning of Whole Natural Product Biosynthetic Gene Clusters Frontiers in Bioengineering and Biotechnology natural product biosynthetic gene cluster heterologous expression sequence-independent cloning direct cloning |
| title | Bioprospecting Through Cloning of Whole Natural Product Biosynthetic Gene Clusters |
| title_full | Bioprospecting Through Cloning of Whole Natural Product Biosynthetic Gene Clusters |
| title_fullStr | Bioprospecting Through Cloning of Whole Natural Product Biosynthetic Gene Clusters |
| title_full_unstemmed | Bioprospecting Through Cloning of Whole Natural Product Biosynthetic Gene Clusters |
| title_short | Bioprospecting Through Cloning of Whole Natural Product Biosynthetic Gene Clusters |
| title_sort | bioprospecting through cloning of whole natural product biosynthetic gene clusters |
| topic | natural product biosynthetic gene cluster heterologous expression sequence-independent cloning direct cloning |
| url | https://www.frontiersin.org/article/10.3389/fbioe.2020.00526/full |
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