Evaluation of the Cariogenic and Anti-Cariogenic Potential of Human Colostrum and Colostrum-Derived Probiotics: Impact on <i>S. mutans</i> Growth, Biofilm Formation, and <i>L. rhamnosus</i> Growth
Human colostrum (HC) is essential for oral health as it is rich in probiotics that could affect the growth of the cariogenic <i>S. mutans</i> and its biofilm formation; hindering dental caries in advance. In this study, HC was collected from 36 healthy mothers 1–3 days postpartum. The ef...
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MDPI AG
2023-09-01
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| Series: | Life |
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| Online Access: | https://www.mdpi.com/2075-1729/13/9/1869 |
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| author | Samaa A. Zaghloul Sara N. Hashem Safaa R. El-Sayed Mona Shaban E. M. Badawy Sarah I. Bukhari Heba Mohammed Refat M. Selim Omnia Karem M. Riad |
| author_facet | Samaa A. Zaghloul Sara N. Hashem Safaa R. El-Sayed Mona Shaban E. M. Badawy Sarah I. Bukhari Heba Mohammed Refat M. Selim Omnia Karem M. Riad |
| author_sort | Samaa A. Zaghloul |
| collection | DOAJ |
| description | Human colostrum (HC) is essential for oral health as it is rich in probiotics that could affect the growth of the cariogenic <i>S. mutans</i> and its biofilm formation; hindering dental caries in advance. In this study, HC was collected from 36 healthy mothers 1–3 days postpartum. The effect of HC on oral health was carried out by assessing the impact of HC and its derived probiotics’ cell-free supernatants (CFS) on the growth of <i>S. mutans</i> (using modified well diffusion) and its biofilm formation (using microtiter plate assay). Moreover, the effect of whole HC on <i>L. rhamnosus</i>, a probiotic oral bacterium, was examined. Probiotics were isolated and identified phenotypically by API 50 CH carbohydrate fermentation and genotypically by 16S rRNA amplification. The in vitro study revealed that HC has cariogenic activity and is associated with biofilm formation. Biofilm strength was inversely proportional to HC dilution (<i>p</i>-value < 0.0001). Nevertheless, HC and colostrum-derived probiotics improve oral health by inhibiting the growth of caries-inducing <i>S. mutans</i> with lower inhibition to <i>L. rhamnosus</i> probiotics. The CFS of isolated probiotics reduced the biofilm formation via the cariogenic <i>S. mutans</i>. These results are not only promising for caries eradication, but they also highlight the importance of breastfeeding infants from their first hours to shape healthy oral microbiota, protecting them from various diseases including dental caries. |
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| institution | Directory Open Access Journal |
| issn | 2075-1729 |
| language | English |
| last_indexed | 2024-03-10T22:33:35Z |
| publishDate | 2023-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Life |
| spelling | doaj.art-f1f0d5cf1f6e4377b932e577a5f6baf82023-11-19T11:37:26ZengMDPI AGLife2075-17292023-09-01139186910.3390/life13091869Evaluation of the Cariogenic and Anti-Cariogenic Potential of Human Colostrum and Colostrum-Derived Probiotics: Impact on <i>S. mutans</i> Growth, Biofilm Formation, and <i>L. rhamnosus</i> GrowthSamaa A. Zaghloul0Sara N. Hashem1Safaa R. El-Sayed2Mona Shaban E. M. Badawy3Sarah I. Bukhari4Heba Mohammed Refat M. Selim5Omnia Karem M. Riad6Department of Operative Dentistry, Faculty of Dental Medicine for Girls, Al-Azhar University, Cairo 11765, EgyptDepartment of Pedodontics and Oral Health, Faculty of Dental Medicine for Girls, Al-Azhar University, Cairo 11651, EgyptDepartment of Pedodontics and Oral Health, Faculty of Dental Medicine for Girls, Al-Azhar University, Cairo 11651, EgyptDepartment of Microbiology and Immunology, Faculty of Pharmacy for Girls, Al-Azhar University, Cairo 11884, EgyptDepartment of Pharmaceutics, College of Pharmacy, King Saud University, Riyadh 11451, Saudi ArabiaDepartment of Microbiology and Immunology, Faculty of Pharmacy for Girls, Al-Azhar University, Cairo 11884, EgyptDepartment of Microbiology and Immunology, Faculty of Pharmacy for Girls, Al-Azhar University, Cairo 11884, EgyptHuman colostrum (HC) is essential for oral health as it is rich in probiotics that could affect the growth of the cariogenic <i>S. mutans</i> and its biofilm formation; hindering dental caries in advance. In this study, HC was collected from 36 healthy mothers 1–3 days postpartum. The effect of HC on oral health was carried out by assessing the impact of HC and its derived probiotics’ cell-free supernatants (CFS) on the growth of <i>S. mutans</i> (using modified well diffusion) and its biofilm formation (using microtiter plate assay). Moreover, the effect of whole HC on <i>L. rhamnosus</i>, a probiotic oral bacterium, was examined. Probiotics were isolated and identified phenotypically by API 50 CH carbohydrate fermentation and genotypically by 16S rRNA amplification. The in vitro study revealed that HC has cariogenic activity and is associated with biofilm formation. Biofilm strength was inversely proportional to HC dilution (<i>p</i>-value < 0.0001). Nevertheless, HC and colostrum-derived probiotics improve oral health by inhibiting the growth of caries-inducing <i>S. mutans</i> with lower inhibition to <i>L. rhamnosus</i> probiotics. The CFS of isolated probiotics reduced the biofilm formation via the cariogenic <i>S. mutans</i>. These results are not only promising for caries eradication, but they also highlight the importance of breastfeeding infants from their first hours to shape healthy oral microbiota, protecting them from various diseases including dental caries.https://www.mdpi.com/2075-1729/13/9/1869biofilmbreast feedingcell-free supernatantdental carieshuman colostrum<i>L. rhamnosus</i> |
| spellingShingle | Samaa A. Zaghloul Sara N. Hashem Safaa R. El-Sayed Mona Shaban E. M. Badawy Sarah I. Bukhari Heba Mohammed Refat M. Selim Omnia Karem M. Riad Evaluation of the Cariogenic and Anti-Cariogenic Potential of Human Colostrum and Colostrum-Derived Probiotics: Impact on <i>S. mutans</i> Growth, Biofilm Formation, and <i>L. rhamnosus</i> Growth Life biofilm breast feeding cell-free supernatant dental caries human colostrum <i>L. rhamnosus</i> |
| title | Evaluation of the Cariogenic and Anti-Cariogenic Potential of Human Colostrum and Colostrum-Derived Probiotics: Impact on <i>S. mutans</i> Growth, Biofilm Formation, and <i>L. rhamnosus</i> Growth |
| title_full | Evaluation of the Cariogenic and Anti-Cariogenic Potential of Human Colostrum and Colostrum-Derived Probiotics: Impact on <i>S. mutans</i> Growth, Biofilm Formation, and <i>L. rhamnosus</i> Growth |
| title_fullStr | Evaluation of the Cariogenic and Anti-Cariogenic Potential of Human Colostrum and Colostrum-Derived Probiotics: Impact on <i>S. mutans</i> Growth, Biofilm Formation, and <i>L. rhamnosus</i> Growth |
| title_full_unstemmed | Evaluation of the Cariogenic and Anti-Cariogenic Potential of Human Colostrum and Colostrum-Derived Probiotics: Impact on <i>S. mutans</i> Growth, Biofilm Formation, and <i>L. rhamnosus</i> Growth |
| title_short | Evaluation of the Cariogenic and Anti-Cariogenic Potential of Human Colostrum and Colostrum-Derived Probiotics: Impact on <i>S. mutans</i> Growth, Biofilm Formation, and <i>L. rhamnosus</i> Growth |
| title_sort | evaluation of the cariogenic and anti cariogenic potential of human colostrum and colostrum derived probiotics impact on i s mutans i growth biofilm formation and i l rhamnosus i growth |
| topic | biofilm breast feeding cell-free supernatant dental caries human colostrum <i>L. rhamnosus</i> |
| url | https://www.mdpi.com/2075-1729/13/9/1869 |
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