<i>Pseudorogneria libanotica</i> Intraspecific Genetic Polymorphism Revealed by Fluorescence In Situ Hybridization with Newly Identified Tandem Repeats and Wheat Single-Copy Gene Probes
The genus <i>Pseudoroegneria</i> (Nevski) Löve (Triticeae, Poaceae) with its genome abbreviated ‘St’ accounts for more than 60% of perennial Triticeae species. The diploid species <i>Psudoroegneria libanotica</i> (2n = 14) contains the most ancient St genome. Therefore, inves...
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MDPI AG
2022-11-01
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| Series: | International Journal of Molecular Sciences |
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| author | Dandan Wu Namei Yang Qian Xiang Mingkun Zhu Zhongyan Fang Wen Zheng Jiale Lu Lina Sha Xing Fan Yiran Cheng Yi Wang Houyang Kang Haiqin Zhang Yonghong Zhou |
| author_facet | Dandan Wu Namei Yang Qian Xiang Mingkun Zhu Zhongyan Fang Wen Zheng Jiale Lu Lina Sha Xing Fan Yiran Cheng Yi Wang Houyang Kang Haiqin Zhang Yonghong Zhou |
| author_sort | Dandan Wu |
| collection | DOAJ |
| description | The genus <i>Pseudoroegneria</i> (Nevski) Löve (Triticeae, Poaceae) with its genome abbreviated ‘St’ accounts for more than 60% of perennial Triticeae species. The diploid species <i>Psudoroegneria libanotica</i> (2n = 14) contains the most ancient St genome. Therefore, investigating its chromosomes could provide some fundamental information required for subsequent studies of St genome evolution. Here, 24 wheat cDNA probes covering seven chromosome groups were mapped in <i>P. libanotica</i> to distinguish homoelogous chromosomes, and newly identified tandem repeats were performed to differentiate seven chromosome pairs. Using these probes, we investigated intraspecific population chromosomal polymorphism of <i>P. libanotica</i>. We found that (i) a duplicated fragment of the 5St long arm was inserted into the short arm of 2St; (ii) asymmetrical fluorescence in situ hybridization (FISH) hybridization signals among 2St, 5St, and 7St homologous chromosome pairs; and (iii) intraspecific population of polymorphism in <i>P. libanotica.</i> These observations established the integrated molecular karyotype of <i>P. libanotica.</i> Moreover, we suggested heterozygosity due to outcrossing habit and adaptation to the local climate of <i>P. libanotica.</i> Specifically, the generated STlib_96 and STlib_98 repeats showed no cross-hybridization signals with wheat chromosomes, suggesting that they are valuable for identifying alien chromosomes or introgressed fragments of wild relatives in wheat. |
| first_indexed | 2024-03-09T17:46:59Z |
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| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T17:46:59Z |
| publishDate | 2022-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-f1f1b2044d2d4dcda8250c4c8c273ed62023-11-24T11:08:28ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-11-0123231481810.3390/ijms232314818<i>Pseudorogneria libanotica</i> Intraspecific Genetic Polymorphism Revealed by Fluorescence In Situ Hybridization with Newly Identified Tandem Repeats and Wheat Single-Copy Gene ProbesDandan Wu0Namei Yang1Qian Xiang2Mingkun Zhu3Zhongyan Fang4Wen Zheng5Jiale Lu6Lina Sha7Xing Fan8Yiran Cheng9Yi Wang10Houyang Kang11Haiqin Zhang12Yonghong Zhou13State Key Laboratory of Crop Genetic Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu 611130, ChinaTriticeae Research Institute, Sichuan Agricultural University, Chengdu 611130, ChinaTriticeae Research Institute, Sichuan Agricultural University, Chengdu 611130, ChinaTriticeae Research Institute, Sichuan Agricultural University, Chengdu 611130, ChinaTriticeae Research Institute, Sichuan Agricultural University, Chengdu 611130, ChinaTriticeae Research Institute, Sichuan Agricultural University, Chengdu 611130, ChinaState Key Laboratory of Crop Genetic Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu 611130, ChinaState Key Laboratory of Crop Genetic Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu 611130, ChinaState Key Laboratory of Crop Genetic Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu 611130, ChinaState Key Laboratory of Crop Genetic Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu 611130, ChinaState Key Laboratory of Crop Genetic Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu 611130, ChinaState Key Laboratory of Crop Genetic Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu 611130, ChinaTriticeae Research Institute, Sichuan Agricultural University, Chengdu 611130, ChinaState Key Laboratory of Crop Genetic Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu 611130, ChinaThe genus <i>Pseudoroegneria</i> (Nevski) Löve (Triticeae, Poaceae) with its genome abbreviated ‘St’ accounts for more than 60% of perennial Triticeae species. The diploid species <i>Psudoroegneria libanotica</i> (2n = 14) contains the most ancient St genome. Therefore, investigating its chromosomes could provide some fundamental information required for subsequent studies of St genome evolution. Here, 24 wheat cDNA probes covering seven chromosome groups were mapped in <i>P. libanotica</i> to distinguish homoelogous chromosomes, and newly identified tandem repeats were performed to differentiate seven chromosome pairs. Using these probes, we investigated intraspecific population chromosomal polymorphism of <i>P. libanotica</i>. We found that (i) a duplicated fragment of the 5St long arm was inserted into the short arm of 2St; (ii) asymmetrical fluorescence in situ hybridization (FISH) hybridization signals among 2St, 5St, and 7St homologous chromosome pairs; and (iii) intraspecific population of polymorphism in <i>P. libanotica.</i> These observations established the integrated molecular karyotype of <i>P. libanotica.</i> Moreover, we suggested heterozygosity due to outcrossing habit and adaptation to the local climate of <i>P. libanotica.</i> Specifically, the generated STlib_96 and STlib_98 repeats showed no cross-hybridization signals with wheat chromosomes, suggesting that they are valuable for identifying alien chromosomes or introgressed fragments of wild relatives in wheat.https://www.mdpi.com/1422-0067/23/23/14818fluorescence in situ hybridizationhomoeologous chromosometandem repeatcytogenetic karyotypegenetic polymorphism<i>Pseudoroegneria libanotica</i> |
| spellingShingle | Dandan Wu Namei Yang Qian Xiang Mingkun Zhu Zhongyan Fang Wen Zheng Jiale Lu Lina Sha Xing Fan Yiran Cheng Yi Wang Houyang Kang Haiqin Zhang Yonghong Zhou <i>Pseudorogneria libanotica</i> Intraspecific Genetic Polymorphism Revealed by Fluorescence In Situ Hybridization with Newly Identified Tandem Repeats and Wheat Single-Copy Gene Probes International Journal of Molecular Sciences fluorescence in situ hybridization homoeologous chromosome tandem repeat cytogenetic karyotype genetic polymorphism <i>Pseudoroegneria libanotica</i> |
| title | <i>Pseudorogneria libanotica</i> Intraspecific Genetic Polymorphism Revealed by Fluorescence In Situ Hybridization with Newly Identified Tandem Repeats and Wheat Single-Copy Gene Probes |
| title_full | <i>Pseudorogneria libanotica</i> Intraspecific Genetic Polymorphism Revealed by Fluorescence In Situ Hybridization with Newly Identified Tandem Repeats and Wheat Single-Copy Gene Probes |
| title_fullStr | <i>Pseudorogneria libanotica</i> Intraspecific Genetic Polymorphism Revealed by Fluorescence In Situ Hybridization with Newly Identified Tandem Repeats and Wheat Single-Copy Gene Probes |
| title_full_unstemmed | <i>Pseudorogneria libanotica</i> Intraspecific Genetic Polymorphism Revealed by Fluorescence In Situ Hybridization with Newly Identified Tandem Repeats and Wheat Single-Copy Gene Probes |
| title_short | <i>Pseudorogneria libanotica</i> Intraspecific Genetic Polymorphism Revealed by Fluorescence In Situ Hybridization with Newly Identified Tandem Repeats and Wheat Single-Copy Gene Probes |
| title_sort | i pseudorogneria libanotica i intraspecific genetic polymorphism revealed by fluorescence in situ hybridization with newly identified tandem repeats and wheat single copy gene probes |
| topic | fluorescence in situ hybridization homoeologous chromosome tandem repeat cytogenetic karyotype genetic polymorphism <i>Pseudoroegneria libanotica</i> |
| url | https://www.mdpi.com/1422-0067/23/23/14818 |
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