Store-operated calcium entry involves in regulating the hyperpermeability of endothelial cells induced by ox-LDL
Objective To explore the effects of store-operated calcium entry(SOCE) on oxidized low-density lipoprotein (ox-LDL)-induced hyperpermeability in human umbilical vein endothelial cells(HUVECs). Methods HUVECs at passage 3 were divided into the control group and the ox-LDL(10, 25, 50 and 100 μg/mL) in...
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Editorial Office of Journal of Army Medical University
2023-02-01
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| Series: | 陆军军医大学学报 |
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| Online Access: | http://aammt.tmmu.edu.cn/Upload/rhtml/202207164.htm |
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| author | KANG Yue TAI Suyu LI Maowei YU Linjun WANG Lianyou |
| author_facet | KANG Yue TAI Suyu LI Maowei YU Linjun WANG Lianyou |
| author_sort | KANG Yue |
| collection | DOAJ |
| description | Objective To explore the effects of store-operated calcium entry(SOCE) on oxidized low-density lipoprotein (ox-LDL)-induced hyperpermeability in human umbilical vein endothelial cells(HUVECs). Methods HUVECs at passage 3 were divided into the control group and the ox-LDL(10, 25, 50 and 100 μg/mL) intervention groups (n=3) for the examination of transendothelial electrical resistance (TER). HUVECs cultured in slides were randomly divided into the control group, the ox-LDL 24 h group and the ox-LDL 48 h group. The distribution of F-actin and VE-cadherin were detected by immunofluorescence, and the protein content of VE-cadherin was detected by Western blotting. Incubated with calcium fluorescence probe (Fluo-3/AM), intracellular free Ca2+ concentration (control group, ox-LDLgroup, ox-LDL+2-APB group) and SOCE (control group, ox-LDL 24 h group, ox-LDL 48 h group) were detected by laser confocal microscopy. Results Low dose of ox-LDL(10, 25 μg/mL) exerted no effect on TER. High dose of ox-LDL (50, 100 μg/mL) also had no effect on TER within 6 h, but after 6 h showed no effect on TER. However, high dose of ox-LDL(50, 100 μg/mL) reduced the decrease of TER in 12 h (P < 0.05), and the decrease was more significant in 24 h and 48 h. After the incubation with ox-LDL(100 μg/mL) 24 h and 48 h, the expression level of VE-cadherin decreased(P < 0.05), while the form and distribution of F-action changed; Intracellular free Ca2+ concentration increased obviously which was interrupted by 2-APB the SOCE inhibitor (P < 0.05), and the 2-APB can decrease the effect of ox-LDL on TER. After incubation with ox-LDL(100 μg/mL) 48 h, the amplitude of SOCE decreased (P < 0.05). The descending artery flow velocity of SOCE showed a slightly decreased trend after incubation with ox-LDL 24 h and a more significant decrease in 48 h(P < 0.05). Conclusion Ox-LDL up-regulate the intracellular free Ca2+ concentration of vascular endothelial cells by extending the duration of SOCE to effect the arrangement of cytoskeleton and cell junction, which increases the cell permeability.
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| first_indexed | 2024-04-10T07:04:27Z |
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| id | doaj.art-f1f96b1326564045b9674d7fa442b070 |
| institution | Directory Open Access Journal |
| issn | 2097-0927 |
| language | zho |
| last_indexed | 2024-04-10T07:04:27Z |
| publishDate | 2023-02-01 |
| publisher | Editorial Office of Journal of Army Medical University |
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| series | 陆军军医大学学报 |
| spelling | doaj.art-f1f96b1326564045b9674d7fa442b0702023-02-27T11:19:55ZzhoEditorial Office of Journal of Army Medical University陆军军医大学学报2097-09272023-02-0145435836510.16016/j.2097-0927.202207164Store-operated calcium entry involves in regulating the hyperpermeability of endothelial cells induced by ox-LDLKANG Yue0TAI Suyu1LI Maowei2YU Linjun3WANG Lianyou4School of Graduate Students, Xinxiang Medical University, Xinxiang, Henan Province, 453003 Department of Cardiology, No. 988 Hospital of Joint Logistic Force of PLA, Zhengzhou, Henan Province, 450042, China Department of Cardiology, No. 988 Hospital of Joint Logistic Force of PLA, Zhengzhou, Henan Province, 450042, ChinaDepartment of Cardiology, No. 988 Hospital of Joint Logistic Force of PLA, Zhengzhou, Henan Province, 450042, ChinaDepartment of Cardiology, No. 988 Hospital of Joint Logistic Force of PLA, Zhengzhou, Henan Province, 450042, ChinaObjective To explore the effects of store-operated calcium entry(SOCE) on oxidized low-density lipoprotein (ox-LDL)-induced hyperpermeability in human umbilical vein endothelial cells(HUVECs). Methods HUVECs at passage 3 were divided into the control group and the ox-LDL(10, 25, 50 and 100 μg/mL) intervention groups (n=3) for the examination of transendothelial electrical resistance (TER). HUVECs cultured in slides were randomly divided into the control group, the ox-LDL 24 h group and the ox-LDL 48 h group. The distribution of F-actin and VE-cadherin were detected by immunofluorescence, and the protein content of VE-cadherin was detected by Western blotting. Incubated with calcium fluorescence probe (Fluo-3/AM), intracellular free Ca2+ concentration (control group, ox-LDLgroup, ox-LDL+2-APB group) and SOCE (control group, ox-LDL 24 h group, ox-LDL 48 h group) were detected by laser confocal microscopy. Results Low dose of ox-LDL(10, 25 μg/mL) exerted no effect on TER. High dose of ox-LDL (50, 100 μg/mL) also had no effect on TER within 6 h, but after 6 h showed no effect on TER. However, high dose of ox-LDL(50, 100 μg/mL) reduced the decrease of TER in 12 h (P < 0.05), and the decrease was more significant in 24 h and 48 h. After the incubation with ox-LDL(100 μg/mL) 24 h and 48 h, the expression level of VE-cadherin decreased(P < 0.05), while the form and distribution of F-action changed; Intracellular free Ca2+ concentration increased obviously which was interrupted by 2-APB the SOCE inhibitor (P < 0.05), and the 2-APB can decrease the effect of ox-LDL on TER. After incubation with ox-LDL(100 μg/mL) 48 h, the amplitude of SOCE decreased (P < 0.05). The descending artery flow velocity of SOCE showed a slightly decreased trend after incubation with ox-LDL 24 h and a more significant decrease in 48 h(P < 0.05). Conclusion Ox-LDL up-regulate the intracellular free Ca2+ concentration of vascular endothelial cells by extending the duration of SOCE to effect the arrangement of cytoskeleton and cell junction, which increases the cell permeability. http://aammt.tmmu.edu.cn/Upload/rhtml/202207164.htmstore-operated calcium entrycytoskeletonoxidized low-density lipoproteinpermeabilityvascular endothelial cells |
| spellingShingle | KANG Yue TAI Suyu LI Maowei YU Linjun WANG Lianyou Store-operated calcium entry involves in regulating the hyperpermeability of endothelial cells induced by ox-LDL 陆军军医大学学报 store-operated calcium entry cytoskeleton oxidized low-density lipoprotein permeability vascular endothelial cells |
| title | Store-operated calcium entry involves in regulating the hyperpermeability of endothelial cells induced by ox-LDL |
| title_full | Store-operated calcium entry involves in regulating the hyperpermeability of endothelial cells induced by ox-LDL |
| title_fullStr | Store-operated calcium entry involves in regulating the hyperpermeability of endothelial cells induced by ox-LDL |
| title_full_unstemmed | Store-operated calcium entry involves in regulating the hyperpermeability of endothelial cells induced by ox-LDL |
| title_short | Store-operated calcium entry involves in regulating the hyperpermeability of endothelial cells induced by ox-LDL |
| title_sort | store operated calcium entry involves in regulating the hyperpermeability of endothelial cells induced by ox ldl |
| topic | store-operated calcium entry cytoskeleton oxidized low-density lipoprotein permeability vascular endothelial cells |
| url | http://aammt.tmmu.edu.cn/Upload/rhtml/202207164.htm |
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