Biosynthesis of (R)‐(+)‐perillyl alcohol by Escherichia coli expressing neryl pyrophosphate synthase
Abstract (R)‐(+)‐perillyl alcohol is widely used in agricultural and anticarcinogenic fields. Microbial production of (R)‐(+)‐perillyl alcohol was investigated in this study. We optimized biosynthesis of (R)‐(+)‐perillyl alcohol in Escherichia coli by using neryl pyrophosphate synthase and NADPH reg...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Wiley-VCH
2022-05-01
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| Series: | Engineering in Life Sciences |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/elsc.202100135 |
| _version_ | 1811338555487682560 |
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| author | Chao Sun Rubing Zhang Congxia Xie |
| author_facet | Chao Sun Rubing Zhang Congxia Xie |
| author_sort | Chao Sun |
| collection | DOAJ |
| description | Abstract (R)‐(+)‐perillyl alcohol is widely used in agricultural and anticarcinogenic fields. Microbial production of (R)‐(+)‐perillyl alcohol was investigated in this study. We optimized biosynthesis of (R)‐(+)‐perillyl alcohol in Escherichia coli by using neryl pyrophosphate synthase and NADPH regeneration. Engineering neryl pyrophosphate (NPP)‐supplied pathway resulted in a 4‐fold improvement of (R)‐(+)‐perillyl alcohol titer. Subsequently, combined engineering of p‐cymene monooxygenase (CymA) expression and module for NADPH regeneration exhibited a 15.4‐fold increase of titer over the initial strain S02. Finally, 453 mg/L (R)‐(+)‐perillyl alcohol was achieved in fed‐batch fermentation, which is the highest (R)‐(+)‐perillyl alcohol titer in E. coli. |
| first_indexed | 2024-04-13T18:12:37Z |
| format | Article |
| id | doaj.art-f1fe5fdcd5bb4e03a1b814d571ddc0f4 |
| institution | Directory Open Access Journal |
| issn | 1618-0240 1618-2863 |
| language | English |
| last_indexed | 2024-04-13T18:12:37Z |
| publishDate | 2022-05-01 |
| publisher | Wiley-VCH |
| record_format | Article |
| series | Engineering in Life Sciences |
| spelling | doaj.art-f1fe5fdcd5bb4e03a1b814d571ddc0f42022-12-22T02:35:49ZengWiley-VCHEngineering in Life Sciences1618-02401618-28632022-05-0122540741610.1002/elsc.202100135Biosynthesis of (R)‐(+)‐perillyl alcohol by Escherichia coli expressing neryl pyrophosphate synthaseChao Sun0Rubing Zhang1Congxia Xie2State Key Laboratory Base of Eco‐Chemical Engineering, College of Chemistry and Molecular Engineering Qingdao University of Science and Technology Qingdao P. R. ChinaCAS Key Laboratory of Bio‐based Materials Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences Qingdao P. R. ChinaState Key Laboratory Base of Eco‐Chemical Engineering, College of Chemistry and Molecular Engineering Qingdao University of Science and Technology Qingdao P. R. ChinaAbstract (R)‐(+)‐perillyl alcohol is widely used in agricultural and anticarcinogenic fields. Microbial production of (R)‐(+)‐perillyl alcohol was investigated in this study. We optimized biosynthesis of (R)‐(+)‐perillyl alcohol in Escherichia coli by using neryl pyrophosphate synthase and NADPH regeneration. Engineering neryl pyrophosphate (NPP)‐supplied pathway resulted in a 4‐fold improvement of (R)‐(+)‐perillyl alcohol titer. Subsequently, combined engineering of p‐cymene monooxygenase (CymA) expression and module for NADPH regeneration exhibited a 15.4‐fold increase of titer over the initial strain S02. Finally, 453 mg/L (R)‐(+)‐perillyl alcohol was achieved in fed‐batch fermentation, which is the highest (R)‐(+)‐perillyl alcohol titer in E. coli.https://doi.org/10.1002/elsc.202100135(R)‐(+)‐perillyl alcoholEscherichia coliNADPH regenerationneryl pyrophosphate synthase |
| spellingShingle | Chao Sun Rubing Zhang Congxia Xie Biosynthesis of (R)‐(+)‐perillyl alcohol by Escherichia coli expressing neryl pyrophosphate synthase Engineering in Life Sciences (R)‐(+)‐perillyl alcohol Escherichia coli NADPH regeneration neryl pyrophosphate synthase |
| title | Biosynthesis of (R)‐(+)‐perillyl alcohol by Escherichia coli expressing neryl pyrophosphate synthase |
| title_full | Biosynthesis of (R)‐(+)‐perillyl alcohol by Escherichia coli expressing neryl pyrophosphate synthase |
| title_fullStr | Biosynthesis of (R)‐(+)‐perillyl alcohol by Escherichia coli expressing neryl pyrophosphate synthase |
| title_full_unstemmed | Biosynthesis of (R)‐(+)‐perillyl alcohol by Escherichia coli expressing neryl pyrophosphate synthase |
| title_short | Biosynthesis of (R)‐(+)‐perillyl alcohol by Escherichia coli expressing neryl pyrophosphate synthase |
| title_sort | biosynthesis of r perillyl alcohol by escherichia coli expressing neryl pyrophosphate synthase |
| topic | (R)‐(+)‐perillyl alcohol Escherichia coli NADPH regeneration neryl pyrophosphate synthase |
| url | https://doi.org/10.1002/elsc.202100135 |
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