Multicentre interlaboratory analysis of routine susceptibility testing with a challenge panel of resistant strains

Multicentre interlaboratory analysis of routine susceptibility testing with a challenge panel of resistant strains

ABSTRACT: Objectives: In order to elaborate a new national challenge panel of resistant Gram-negative bacilli and Gram-positive cocci strains for the validation of routine antimicrobial susceptibility testing (AST) methods, an interlaboratory evaluation was organised. Methods: The results of 12 wel...

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Bibliographic Details
Main Authors: Corentin Deckers, Reza Soleimani, Olivier Denis, Pierre Bogaerts, Catherine Berhin, Hector Rodríguez-Villalobos, Julie Descy, Marie Hallin, Claire Nonhoff, Stefanie Desmet, Koen Magerman, Anne Marie Van den Abeele, Bénédicte Lissoir, Veerle Matheeussen, Kris Vernelen, Te-Din Huang
Format: Article
Language:English
Published: Elsevier 2022-03-01
Series:Journal of Global Antimicrobial Resistance
Subjects:
Interlaboratory assay
Quality control
Multidrug-resistant organisms
Automated susceptibility testing
Disk diffusion
Online Access:http://www.sciencedirect.com/science/article/pii/S2213716522000054
Description
Summary:ABSTRACT: Objectives: In order to elaborate a new national challenge panel of resistant Gram-negative bacilli and Gram-positive cocci strains for the validation of routine antimicrobial susceptibility testing (AST) methods, an interlaboratory evaluation was organised. Methods: The results of 12 well-characterised multidrug-resistant strains tested by nine laboratories using local disk diffusion (DD) and automated AST (AUST) methods were compared with the reference broth microdilution method. Results: Overall categorical agreement ranged from 70% to 100% both for DD and AUST and was >90% for all but one strain for all antibiotics. Conclusion: Our multicentre AST study showed good reproducibility and the panel can be used as national resistant reference strains for routine AST validation.
ISSN:2213-7165

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