Autophagic Survival Precedes Programmed Cell Death in Wheat Seedlings Exposed to Drought Stress
Although studies have shown the concomitant occurrence of autophagic and programmed cell death (PCD) in plants, the relationship between autophagy and PCD and the factors determining this relationship remain unclear. In this study, seedlings of the wheat cultivar Jimai 22 were used to examine the oc...
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| Format: | Article |
| Language: | English |
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MDPI AG
2019-11-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/20/22/5777 |
| _version_ | 1811310637542801408 |
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| author | Yong-Bo Li De-Zhou Cui Xin-Xia Sui Chen Huang Cheng-Yan Huang Qing-Qi Fan Xiu-Sheng Chu |
| author_facet | Yong-Bo Li De-Zhou Cui Xin-Xia Sui Chen Huang Cheng-Yan Huang Qing-Qi Fan Xiu-Sheng Chu |
| author_sort | Yong-Bo Li |
| collection | DOAJ |
| description | Although studies have shown the concomitant occurrence of autophagic and programmed cell death (PCD) in plants, the relationship between autophagy and PCD and the factors determining this relationship remain unclear. In this study, seedlings of the wheat cultivar Jimai 22 were used to examine the occurrence of autophagy and PCD during polyethylene glycol (PEG)-8000-induced drought stress. Autophagy and PCD occurred sequentially, with autophagy at a relatively early stage and PCD at a much later stage. These findings suggest that the duration of drought stress determines the occurrence of PCD following autophagy. Furthermore, the addition of 3-methyladenine (3-MA, an autophagy inhibitor) and the knockdown of <i>autophagy-related gene 6</i> (<i>ATG6</i>) accelerated PEG-8000-induced PCD, respectively, suggesting that inhibition of autophagy also results in PCD under drought stress. Overall, these findings confirm that wheat seedlings undergo autophagic survival under mild drought stress, with subsequent PCD only under severe drought. |
| first_indexed | 2024-04-13T10:02:23Z |
| format | Article |
| id | doaj.art-f2016e1a376f41dd9809dfbfe0fea542 |
| institution | Directory Open Access Journal |
| issn | 1422-0067 |
| language | English |
| last_indexed | 2024-04-13T10:02:23Z |
| publishDate | 2019-11-01 |
| publisher | MDPI AG |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-f2016e1a376f41dd9809dfbfe0fea5422022-12-22T02:51:13ZengMDPI AGInternational Journal of Molecular Sciences1422-00672019-11-012022577710.3390/ijms20225777ijms20225777Autophagic Survival Precedes Programmed Cell Death in Wheat Seedlings Exposed to Drought StressYong-Bo Li0De-Zhou Cui1Xin-Xia Sui2Chen Huang3Cheng-Yan Huang4Qing-Qi Fan5Xiu-Sheng Chu6Crop Research Institute, Shandong Academy of Agricultural Sciences, Jinan 250100, ChinaCrop Research Institute, Shandong Academy of Agricultural Sciences, Jinan 250100, ChinaCrop Research Institute, Shandong Academy of Agricultural Sciences, Jinan 250100, ChinaCrop Research Institute, Shandong Academy of Agricultural Sciences, Jinan 250100, ChinaCrop Research Institute, Shandong Academy of Agricultural Sciences, Jinan 250100, ChinaCrop Research Institute, Shandong Academy of Agricultural Sciences, Jinan 250100, ChinaCrop Research Institute, Shandong Academy of Agricultural Sciences, Jinan 250100, ChinaAlthough studies have shown the concomitant occurrence of autophagic and programmed cell death (PCD) in plants, the relationship between autophagy and PCD and the factors determining this relationship remain unclear. In this study, seedlings of the wheat cultivar Jimai 22 were used to examine the occurrence of autophagy and PCD during polyethylene glycol (PEG)-8000-induced drought stress. Autophagy and PCD occurred sequentially, with autophagy at a relatively early stage and PCD at a much later stage. These findings suggest that the duration of drought stress determines the occurrence of PCD following autophagy. Furthermore, the addition of 3-methyladenine (3-MA, an autophagy inhibitor) and the knockdown of <i>autophagy-related gene 6</i> (<i>ATG6</i>) accelerated PEG-8000-induced PCD, respectively, suggesting that inhibition of autophagy also results in PCD under drought stress. Overall, these findings confirm that wheat seedlings undergo autophagic survival under mild drought stress, with subsequent PCD only under severe drought.https://www.mdpi.com/1422-0067/20/22/5777wheat seedlingsdroughtautophagyprogrammed cell death |
| spellingShingle | Yong-Bo Li De-Zhou Cui Xin-Xia Sui Chen Huang Cheng-Yan Huang Qing-Qi Fan Xiu-Sheng Chu Autophagic Survival Precedes Programmed Cell Death in Wheat Seedlings Exposed to Drought Stress International Journal of Molecular Sciences wheat seedlings drought autophagy programmed cell death |
| title | Autophagic Survival Precedes Programmed Cell Death in Wheat Seedlings Exposed to Drought Stress |
| title_full | Autophagic Survival Precedes Programmed Cell Death in Wheat Seedlings Exposed to Drought Stress |
| title_fullStr | Autophagic Survival Precedes Programmed Cell Death in Wheat Seedlings Exposed to Drought Stress |
| title_full_unstemmed | Autophagic Survival Precedes Programmed Cell Death in Wheat Seedlings Exposed to Drought Stress |
| title_short | Autophagic Survival Precedes Programmed Cell Death in Wheat Seedlings Exposed to Drought Stress |
| title_sort | autophagic survival precedes programmed cell death in wheat seedlings exposed to drought stress |
| topic | wheat seedlings drought autophagy programmed cell death |
| url | https://www.mdpi.com/1422-0067/20/22/5777 |
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