Study of Alzheimer’s Disease-Related Biophysical Kinetics with a Microslit-Embedded Cantilever Sensor in a Liquid Environment
A microsized slit-embedded cantilever sensor (slit cantilever) was fabricated and evaluated as a biosensing platform in a liquid environment. In order to minimize the degradation caused by viscous damping, a 300 × 100 µm2 (length × width) sized cantilever was released by a 5 µm gap-surrounding and v...
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| Format: | Article |
| Language: | English |
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MDPI AG
2017-08-01
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| Series: | Sensors |
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| Online Access: | https://www.mdpi.com/1424-8220/17/8/1819 |
| _version_ | 1798027023629680640 |
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| author | Myung-Sic Chae Jinsik Kim Yong Kyoung Yoo Jeong Hoon Lee Tae Geun Kim Kyo Seon Hwang |
| author_facet | Myung-Sic Chae Jinsik Kim Yong Kyoung Yoo Jeong Hoon Lee Tae Geun Kim Kyo Seon Hwang |
| author_sort | Myung-Sic Chae |
| collection | DOAJ |
| description | A microsized slit-embedded cantilever sensor (slit cantilever) was fabricated and evaluated as a biosensing platform in a liquid environment. In order to minimize the degradation caused by viscous damping, a 300 × 100 µm2 (length × width) sized cantilever was released by a 5 µm gap-surrounding and vibrated by an internal piezoelectric-driven self-actuator. Owing to the structure, when the single side of the slit cantilever was exposed to liquid a significant quality factor (Q = 35) could be achieved. To assess the sensing performance, the slit cantilever was exploited to study the biophysical kinetics related to Aβ peptide. First, the quantification of Aβ peptide with a concentration of 10 pg/mL to 1 μg/mL was performed. The resonant responses exhibited a dynamic range from 100 pg/mL to 100 ng/mL (−56.5 to −774 ΔHz) and a dissociation constant (KD) of binding affinity was calculated as 1.75 nM. Finally, the Aβ self-aggregation associated with AD pathogenesis was monitored by adding monomeric Aβ peptides. As the concentration of added analyte increased from 100 ng/mL to 10 µg/mL, both the frequency shift values (−813 to −1804 ΔHz) and associate time constant increased. These results showed the excellent sensing performance of the slit cantilever overcoming a major drawback in liquid environments to become a promising diagnostic tool candidate. |
| first_indexed | 2024-04-11T18:45:48Z |
| format | Article |
| id | doaj.art-f2033043ca2d433c80ee114462e940e9 |
| institution | Directory Open Access Journal |
| issn | 1424-8220 |
| language | English |
| last_indexed | 2024-04-11T18:45:48Z |
| publishDate | 2017-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Sensors |
| spelling | doaj.art-f2033043ca2d433c80ee114462e940e92022-12-22T04:08:51ZengMDPI AGSensors1424-82202017-08-01178181910.3390/s17081819s17081819Study of Alzheimer’s Disease-Related Biophysical Kinetics with a Microslit-Embedded Cantilever Sensor in a Liquid EnvironmentMyung-Sic Chae0Jinsik Kim1Yong Kyoung Yoo2Jeong Hoon Lee3Tae Geun Kim4Kyo Seon Hwang5Department of Clinical Pharmacology and Therapeutics, College of Medicine, Kyung Hee University, Seoul 02447, KoreaDepartment of Medical Biotechnology, College of Life Science and Biotechnology, Dongguk University, Seoul 10326, KoreaDepartment of Electrical Engineering, Kwangwoon University, Seoul 01897, KoreaDepartment of Electrical Engineering, Kwangwoon University, Seoul 01897, KoreaSchool of Electrical Engineering, Korea University, Seoul 02841, KoreaDepartment of Clinical Pharmacology and Therapeutics, College of Medicine, Kyung Hee University, Seoul 02447, KoreaA microsized slit-embedded cantilever sensor (slit cantilever) was fabricated and evaluated as a biosensing platform in a liquid environment. In order to minimize the degradation caused by viscous damping, a 300 × 100 µm2 (length × width) sized cantilever was released by a 5 µm gap-surrounding and vibrated by an internal piezoelectric-driven self-actuator. Owing to the structure, when the single side of the slit cantilever was exposed to liquid a significant quality factor (Q = 35) could be achieved. To assess the sensing performance, the slit cantilever was exploited to study the biophysical kinetics related to Aβ peptide. First, the quantification of Aβ peptide with a concentration of 10 pg/mL to 1 μg/mL was performed. The resonant responses exhibited a dynamic range from 100 pg/mL to 100 ng/mL (−56.5 to −774 ΔHz) and a dissociation constant (KD) of binding affinity was calculated as 1.75 nM. Finally, the Aβ self-aggregation associated with AD pathogenesis was monitored by adding monomeric Aβ peptides. As the concentration of added analyte increased from 100 ng/mL to 10 µg/mL, both the frequency shift values (−813 to −1804 ΔHz) and associate time constant increased. These results showed the excellent sensing performance of the slit cantilever overcoming a major drawback in liquid environments to become a promising diagnostic tool candidate.https://www.mdpi.com/1424-8220/17/8/1819cantilever sensorbiosensorviscous dampingAlzheimer’s disease |
| spellingShingle | Myung-Sic Chae Jinsik Kim Yong Kyoung Yoo Jeong Hoon Lee Tae Geun Kim Kyo Seon Hwang Study of Alzheimer’s Disease-Related Biophysical Kinetics with a Microslit-Embedded Cantilever Sensor in a Liquid Environment Sensors cantilever sensor biosensor viscous damping Alzheimer’s disease |
| title | Study of Alzheimer’s Disease-Related Biophysical Kinetics with a Microslit-Embedded Cantilever Sensor in a Liquid Environment |
| title_full | Study of Alzheimer’s Disease-Related Biophysical Kinetics with a Microslit-Embedded Cantilever Sensor in a Liquid Environment |
| title_fullStr | Study of Alzheimer’s Disease-Related Biophysical Kinetics with a Microslit-Embedded Cantilever Sensor in a Liquid Environment |
| title_full_unstemmed | Study of Alzheimer’s Disease-Related Biophysical Kinetics with a Microslit-Embedded Cantilever Sensor in a Liquid Environment |
| title_short | Study of Alzheimer’s Disease-Related Biophysical Kinetics with a Microslit-Embedded Cantilever Sensor in a Liquid Environment |
| title_sort | study of alzheimer s disease related biophysical kinetics with a microslit embedded cantilever sensor in a liquid environment |
| topic | cantilever sensor biosensor viscous damping Alzheimer’s disease |
| url | https://www.mdpi.com/1424-8220/17/8/1819 |
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