Effects of occlusal disharmony on cardiac fibrosis, myocyte apoptosis and myocyte oxidative DNA damage in mice.
Occlusal disharmony leads to morphological changes in the hippocampus and osteopenia of the lumbar vertebra and long bones in mice, and causes stress. Various types of stress are associated with increased incidence of cardiovascular disease, but the relationship between occlusal disharmony and cardi...
| Main Authors: | , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2020-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0236547 |
| _version_ | 1798032666717585408 |
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| author | Yuka Yagisawa Kenji Suita Yoshiki Ohnuki Misao Ishikawa Yasumasa Mototani Aiko Ito Ichiro Matsuo Yoshio Hayakawa Megumi Nariyama Daisuke Umeki Yasutake Saeki Yasuharu Amitani Yoshiki Nakamura Hiroshi Tomonari Satoshi Okumura |
| author_facet | Yuka Yagisawa Kenji Suita Yoshiki Ohnuki Misao Ishikawa Yasumasa Mototani Aiko Ito Ichiro Matsuo Yoshio Hayakawa Megumi Nariyama Daisuke Umeki Yasutake Saeki Yasuharu Amitani Yoshiki Nakamura Hiroshi Tomonari Satoshi Okumura |
| author_sort | Yuka Yagisawa |
| collection | DOAJ |
| description | Occlusal disharmony leads to morphological changes in the hippocampus and osteopenia of the lumbar vertebra and long bones in mice, and causes stress. Various types of stress are associated with increased incidence of cardiovascular disease, but the relationship between occlusal disharmony and cardiovascular disease remain poorly understood. Therefore, in this work, we examined the effects of occlusal disharmony on cardiac homeostasis in bite-opening (BO) mice, in which a 0.7 mm space was introduced by cementing a suitable applicance onto the mandibular incisior. We first examined the effects of BO on the level of serum corticosterone, a key biomarker for stress, and on heart rate variability at 14 days after BO treatment, compared with baseline. BO treatment increased serum corticosterone levels by approximately 3.6-fold and the low frequency/high frequency ratio, an index of sympathetic nervous activity, was significantly increased by approximately 4-fold by the BO treatment. We then examined the effects of BO treatment on cardiac homeostasis in mice treated or not treated with the non-selective β-blocker propranolol for 2 weeks. Cardiac function was significantly decreased in the BO group compared to the control group, but propranolol ameliorated the dysfunction. Cardiac fibrosis, myocyte apoptosis and myocyte oxidative DNA damage were significantly increased in the BO group, but propranolol blocked these changes. The BO-induced cardiac dysfunction was associated with increased phospholamban phosphorylation at threonine-17 and serine-16, as well as inhibition of Akt/mTOR signaling and autophagic flux. These data suggest that occlusal disharmony might affect cardiac homeostasis via alteration of the autonomic nervous system. |
| first_indexed | 2024-04-11T20:16:29Z |
| format | Article |
| id | doaj.art-f20604e45c5c4ae682ba0e64004f0051 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-11T20:16:29Z |
| publishDate | 2020-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-f20604e45c5c4ae682ba0e64004f00512022-12-22T04:04:57ZengPublic Library of Science (PLoS)PLoS ONE1932-62032020-01-01157e023654710.1371/journal.pone.0236547Effects of occlusal disharmony on cardiac fibrosis, myocyte apoptosis and myocyte oxidative DNA damage in mice.Yuka YagisawaKenji SuitaYoshiki OhnukiMisao IshikawaYasumasa MototaniAiko ItoIchiro MatsuoYoshio HayakawaMegumi NariyamaDaisuke UmekiYasutake SaekiYasuharu AmitaniYoshiki NakamuraHiroshi TomonariSatoshi OkumuraOcclusal disharmony leads to morphological changes in the hippocampus and osteopenia of the lumbar vertebra and long bones in mice, and causes stress. Various types of stress are associated with increased incidence of cardiovascular disease, but the relationship between occlusal disharmony and cardiovascular disease remain poorly understood. Therefore, in this work, we examined the effects of occlusal disharmony on cardiac homeostasis in bite-opening (BO) mice, in which a 0.7 mm space was introduced by cementing a suitable applicance onto the mandibular incisior. We first examined the effects of BO on the level of serum corticosterone, a key biomarker for stress, and on heart rate variability at 14 days after BO treatment, compared with baseline. BO treatment increased serum corticosterone levels by approximately 3.6-fold and the low frequency/high frequency ratio, an index of sympathetic nervous activity, was significantly increased by approximately 4-fold by the BO treatment. We then examined the effects of BO treatment on cardiac homeostasis in mice treated or not treated with the non-selective β-blocker propranolol for 2 weeks. Cardiac function was significantly decreased in the BO group compared to the control group, but propranolol ameliorated the dysfunction. Cardiac fibrosis, myocyte apoptosis and myocyte oxidative DNA damage were significantly increased in the BO group, but propranolol blocked these changes. The BO-induced cardiac dysfunction was associated with increased phospholamban phosphorylation at threonine-17 and serine-16, as well as inhibition of Akt/mTOR signaling and autophagic flux. These data suggest that occlusal disharmony might affect cardiac homeostasis via alteration of the autonomic nervous system.https://doi.org/10.1371/journal.pone.0236547 |
| spellingShingle | Yuka Yagisawa Kenji Suita Yoshiki Ohnuki Misao Ishikawa Yasumasa Mototani Aiko Ito Ichiro Matsuo Yoshio Hayakawa Megumi Nariyama Daisuke Umeki Yasutake Saeki Yasuharu Amitani Yoshiki Nakamura Hiroshi Tomonari Satoshi Okumura Effects of occlusal disharmony on cardiac fibrosis, myocyte apoptosis and myocyte oxidative DNA damage in mice. PLoS ONE |
| title | Effects of occlusal disharmony on cardiac fibrosis, myocyte apoptosis and myocyte oxidative DNA damage in mice. |
| title_full | Effects of occlusal disharmony on cardiac fibrosis, myocyte apoptosis and myocyte oxidative DNA damage in mice. |
| title_fullStr | Effects of occlusal disharmony on cardiac fibrosis, myocyte apoptosis and myocyte oxidative DNA damage in mice. |
| title_full_unstemmed | Effects of occlusal disharmony on cardiac fibrosis, myocyte apoptosis and myocyte oxidative DNA damage in mice. |
| title_short | Effects of occlusal disharmony on cardiac fibrosis, myocyte apoptosis and myocyte oxidative DNA damage in mice. |
| title_sort | effects of occlusal disharmony on cardiac fibrosis myocyte apoptosis and myocyte oxidative dna damage in mice |
| url | https://doi.org/10.1371/journal.pone.0236547 |
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