Establishment of analytical method for quantification of anti-inflammatory agents co-nanoencapsulated and its application to physicochemical development and characterization of lipid-core nanocapsules
Anti-inflammatory agents may be alternatives for the treatment of Alzheimer's disease. Then, we propose the nanotechnology as a strategy to curcumin and meloxicam co-nanoencapsulation for neurodegenerative diseases treatment. In this study, we developed and validated an analytical method by HPL...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2020-01-01
|
| Series: | Arabian Journal of Chemistry |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1878535218301187 |
| _version_ | 1811268449888894976 |
|---|---|
| author | Kelly Ayumi Nakama Renata Bem dos Santos Carlos Eduardo da Rosa Silva Jessica Cristina Izoton Anne Suely Pinto Savall Maria Eduarda Ziani Gutirrez Silvane Souza Roman Cristiane Luchese Simone Pinton Sandra Elisa Haas |
| author_facet | Kelly Ayumi Nakama Renata Bem dos Santos Carlos Eduardo da Rosa Silva Jessica Cristina Izoton Anne Suely Pinto Savall Maria Eduarda Ziani Gutirrez Silvane Souza Roman Cristiane Luchese Simone Pinton Sandra Elisa Haas |
| author_sort | Kelly Ayumi Nakama |
| collection | DOAJ |
| description | Anti-inflammatory agents may be alternatives for the treatment of Alzheimer's disease. Then, we propose the nanotechnology as a strategy to curcumin and meloxicam co-nanoencapsulation for neurodegenerative diseases treatment. In this study, we developed and validated an analytical method by HPLC-DAD for simultaneous quantification of curcumin and meloxicam in the same formulation. We applied this method to characterize curcumin, meloxican and curcumin plus meloxican loaded lipid-core nanocapsules (LNC): drug content, encapsulation efficiency, photostability and drug's distribution. Additionally, toxicity levels of the nanoparticles were evaluated in vivo (mice). Therefore a C18-RP column was used with a guard column packed with the same material as the stationary phase. Acetonitrile: methanol: water: triethylamine (52:5:43:0.3 v/v/v/v), was used as the mobile phase in the 1 mL min−1 flow. Detection was 424 nm (curcumin) and 365 nm (meloxicam). The method was established in accordance with the current national and international guidelines, showing good linearity (r2 > 0.999). The formulations showed exclusively nanosized particles, negative zeta potential and slightly acidic pH, with content and encapsulation rate close to 100%. The distributions of meloxicam and curcumin in the LNC (alone or co-encapsulated) were type III and type VI, respectively. LNC were able to protect both drugs against UVA degradation. In vivo experiments showed no toxicity in relation to the parameters determined of all LNC evaluated in mice. In this work, we showed the possibility to carry meloxicam and curcumin in LNC with good technological characteristics and without toxicity in vivo with prospects for the treatment of Alzheimer's disease in sequential pharmacological studies. Keywords: Lipid-core nanocapsules, HPLC-PDA, Alzheimer’s disease, Curcumin, Meloxicam |
| first_indexed | 2024-04-12T21:23:06Z |
| format | Article |
| id | doaj.art-f20669f71d0e4f57b4ab0a36f4a5700f |
| institution | Directory Open Access Journal |
| issn | 1878-5352 |
| language | English |
| last_indexed | 2024-04-12T21:23:06Z |
| publishDate | 2020-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Arabian Journal of Chemistry |
| spelling | doaj.art-f20669f71d0e4f57b4ab0a36f4a5700f2022-12-22T03:16:15ZengElsevierArabian Journal of Chemistry1878-53522020-01-0113124562469Establishment of analytical method for quantification of anti-inflammatory agents co-nanoencapsulated and its application to physicochemical development and characterization of lipid-core nanocapsulesKelly Ayumi Nakama0Renata Bem dos Santos1Carlos Eduardo da Rosa Silva2Jessica Cristina Izoton3Anne Suely Pinto Savall4Maria Eduarda Ziani Gutirrez5Silvane Souza Roman6Cristiane Luchese7Simone Pinton8Sandra Elisa Haas9Programa de Pós-Graduação em Ciências Farmacêuticas, Universidade Federal do Pampa, UNIPAMPA, Uruguaiana, BrazilPrograma de Pós-Graduação em Ciências Farmacêuticas, Universidade Federal do Pampa, UNIPAMPA, Uruguaiana, BrazilPrograma de Pós-Graduação em Ciências Farmacêuticas, Universidade Federal do Pampa, UNIPAMPA, Uruguaiana, BrazilPrograma de Pós-Graduação em Ciências Farmacêuticas, Universidade Federal do Pampa, UNIPAMPA, Uruguaiana, BrazilPrograma de Pós-Graduação em Bioquímica, Universidade Federal do Pampa, UNIPAMPA, Uruguaiana, BrazilPrograma de Pós-Graduação em Bioquímica, Universidade Federal do Pampa, UNIPAMPA, Uruguaiana, BrazilUniversidade Regional Integrada do Alto Uruguai e das Missões, Campus Erechim, Erechim, BrazilPrograma de Pós-Graduação em Bioquímica e Bioprospecção, Laboratório de Pesquisa em Farmacologia Bioquímica, Centro de Ciências Químicas, Farmacêuticas e de Alimentos, Universidade Federal de Pelotas, Pelotas, BrazilPrograma de Pós-Graduação em Bioquímica, Universidade Federal do Pampa, UNIPAMPA, Uruguaiana, BrazilPrograma de Pós-Graduação em Ciências Farmacêuticas, Universidade Federal do Pampa, UNIPAMPA, Uruguaiana, Brazil; Corresponding author at: Curso de Farmácia, Universidade Federal do Pampa, UNIPAMPA, Uruguaiana, Brazil.Anti-inflammatory agents may be alternatives for the treatment of Alzheimer's disease. Then, we propose the nanotechnology as a strategy to curcumin and meloxicam co-nanoencapsulation for neurodegenerative diseases treatment. In this study, we developed and validated an analytical method by HPLC-DAD for simultaneous quantification of curcumin and meloxicam in the same formulation. We applied this method to characterize curcumin, meloxican and curcumin plus meloxican loaded lipid-core nanocapsules (LNC): drug content, encapsulation efficiency, photostability and drug's distribution. Additionally, toxicity levels of the nanoparticles were evaluated in vivo (mice). Therefore a C18-RP column was used with a guard column packed with the same material as the stationary phase. Acetonitrile: methanol: water: triethylamine (52:5:43:0.3 v/v/v/v), was used as the mobile phase in the 1 mL min−1 flow. Detection was 424 nm (curcumin) and 365 nm (meloxicam). The method was established in accordance with the current national and international guidelines, showing good linearity (r2 > 0.999). The formulations showed exclusively nanosized particles, negative zeta potential and slightly acidic pH, with content and encapsulation rate close to 100%. The distributions of meloxicam and curcumin in the LNC (alone or co-encapsulated) were type III and type VI, respectively. LNC were able to protect both drugs against UVA degradation. In vivo experiments showed no toxicity in relation to the parameters determined of all LNC evaluated in mice. In this work, we showed the possibility to carry meloxicam and curcumin in LNC with good technological characteristics and without toxicity in vivo with prospects for the treatment of Alzheimer's disease in sequential pharmacological studies. Keywords: Lipid-core nanocapsules, HPLC-PDA, Alzheimer’s disease, Curcumin, Meloxicamhttp://www.sciencedirect.com/science/article/pii/S1878535218301187 |
| spellingShingle | Kelly Ayumi Nakama Renata Bem dos Santos Carlos Eduardo da Rosa Silva Jessica Cristina Izoton Anne Suely Pinto Savall Maria Eduarda Ziani Gutirrez Silvane Souza Roman Cristiane Luchese Simone Pinton Sandra Elisa Haas Establishment of analytical method for quantification of anti-inflammatory agents co-nanoencapsulated and its application to physicochemical development and characterization of lipid-core nanocapsules Arabian Journal of Chemistry |
| title | Establishment of analytical method for quantification of anti-inflammatory agents co-nanoencapsulated and its application to physicochemical development and characterization of lipid-core nanocapsules |
| title_full | Establishment of analytical method for quantification of anti-inflammatory agents co-nanoencapsulated and its application to physicochemical development and characterization of lipid-core nanocapsules |
| title_fullStr | Establishment of analytical method for quantification of anti-inflammatory agents co-nanoencapsulated and its application to physicochemical development and characterization of lipid-core nanocapsules |
| title_full_unstemmed | Establishment of analytical method for quantification of anti-inflammatory agents co-nanoencapsulated and its application to physicochemical development and characterization of lipid-core nanocapsules |
| title_short | Establishment of analytical method for quantification of anti-inflammatory agents co-nanoencapsulated and its application to physicochemical development and characterization of lipid-core nanocapsules |
| title_sort | establishment of analytical method for quantification of anti inflammatory agents co nanoencapsulated and its application to physicochemical development and characterization of lipid core nanocapsules |
| url | http://www.sciencedirect.com/science/article/pii/S1878535218301187 |
| work_keys_str_mv | AT kellyayuminakama establishmentofanalyticalmethodforquantificationofantiinflammatoryagentsconanoencapsulatedanditsapplicationtophysicochemicaldevelopmentandcharacterizationoflipidcorenanocapsules AT renatabemdossantos establishmentofanalyticalmethodforquantificationofantiinflammatoryagentsconanoencapsulatedanditsapplicationtophysicochemicaldevelopmentandcharacterizationoflipidcorenanocapsules AT carloseduardodarosasilva establishmentofanalyticalmethodforquantificationofantiinflammatoryagentsconanoencapsulatedanditsapplicationtophysicochemicaldevelopmentandcharacterizationoflipidcorenanocapsules AT jessicacristinaizoton establishmentofanalyticalmethodforquantificationofantiinflammatoryagentsconanoencapsulatedanditsapplicationtophysicochemicaldevelopmentandcharacterizationoflipidcorenanocapsules AT annesuelypintosavall establishmentofanalyticalmethodforquantificationofantiinflammatoryagentsconanoencapsulatedanditsapplicationtophysicochemicaldevelopmentandcharacterizationoflipidcorenanocapsules AT mariaeduardazianigutirrez establishmentofanalyticalmethodforquantificationofantiinflammatoryagentsconanoencapsulatedanditsapplicationtophysicochemicaldevelopmentandcharacterizationoflipidcorenanocapsules AT silvanesouzaroman establishmentofanalyticalmethodforquantificationofantiinflammatoryagentsconanoencapsulatedanditsapplicationtophysicochemicaldevelopmentandcharacterizationoflipidcorenanocapsules AT cristianeluchese establishmentofanalyticalmethodforquantificationofantiinflammatoryagentsconanoencapsulatedanditsapplicationtophysicochemicaldevelopmentandcharacterizationoflipidcorenanocapsules AT simonepinton establishmentofanalyticalmethodforquantificationofantiinflammatoryagentsconanoencapsulatedanditsapplicationtophysicochemicaldevelopmentandcharacterizationoflipidcorenanocapsules AT sandraelisahaas establishmentofanalyticalmethodforquantificationofantiinflammatoryagentsconanoencapsulatedanditsapplicationtophysicochemicaldevelopmentandcharacterizationoflipidcorenanocapsules |