Comparison of different PCR-based protocols for detection of Roundup Ready soybean
Genetically modified organisms have become a part of our environment and food chain. Roundup Ready soybean is at the moment the most frequent one that man can meet. National regulations require careful monitoring and detection of GMOs. We present in this investigation comparison of several protocols...
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| Format: | Article |
| Language: | English |
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Czech Academy of Agricultural Sciences
2002-03-01
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| Series: | Czech Journal of Genetics and Plant Breeding |
| Subjects: | |
| Online Access: | https://cjgpb.agriculturejournals.cz/artkey/cjg-200201-0007_comparison-of-different-pcr-based-protocols-for-detection-of-roundup-ready-soybean.php |
| _version_ | 1797899576422694912 |
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| author | J. Ovesná L. Dedičová J. Horáček E. Sadilová L. Kučera L. Měsková |
| author_facet | J. Ovesná L. Dedičová J. Horáček E. Sadilová L. Kučera L. Měsková |
| author_sort | J. Ovesná |
| collection | DOAJ |
| description | Genetically modified organisms have become a part of our environment and food chain. Roundup Ready soybean is at the moment the most frequent one that man can meet. National regulations require careful monitoring and detection of GMOs. We present in this investigation comparison of several protocols and individual steps, which are included in the whole detection procedure. Currently used CTAB based protocol is suitable for DNA isolation from the green plant tissue but also from the flour. Lectin coding sequence specific primers were suitable for soybean DNA detection unlike leu-tRNA gene specific primers. Only one of the tested CaMV sequence specific primer pairs did not amplify unspecific products under condition tested. NOS terminator specific primer pair was found suitable for routine screening. EPSPS gene was detected using two different protocols; the same primers did not identify other Roundup Ready GM crops. |
| first_indexed | 2024-04-10T08:32:08Z |
| format | Article |
| id | doaj.art-f20babbe7e644fcf9af20aae01f01746 |
| institution | Directory Open Access Journal |
| issn | 1212-1975 1805-9325 |
| language | English |
| last_indexed | 2024-04-10T08:32:08Z |
| publishDate | 2002-03-01 |
| publisher | Czech Academy of Agricultural Sciences |
| record_format | Article |
| series | Czech Journal of Genetics and Plant Breeding |
| spelling | doaj.art-f20babbe7e644fcf9af20aae01f017462023-02-23T03:28:54ZengCzech Academy of Agricultural SciencesCzech Journal of Genetics and Plant Breeding1212-19751805-93252002-03-01381556310.17221/6111-CJGPBcjg-200201-0007Comparison of different PCR-based protocols for detection of Roundup Ready soybeanJ. Ovesná0L. Dedičová1J. Horáček2E. Sadilová3L. Kučera4L. Měsková5Research Institute of Crop Production - Division of Genetics and Plant Breeding, Prague-Ruzyně, Czech RepublicResearch Institute of Crop Production - Division of Genetics and Plant Breeding, Prague-Ruzyně, Czech RepublicResearch Institute of Crop Production - Division of Genetics and Plant Breeding, Prague-Ruzyně, Czech RepublicResearch Institute of Crop Production - Division of Genetics and Plant Breeding, Prague-Ruzyně, Czech RepublicResearch Institute of Crop Production - Division of Genetics and Plant Breeding, Prague-Ruzyně, Czech RepublicResearch Institute of Crop Production - Division of Genetics and Plant Breeding, Prague-Ruzyně, Czech RepublicGenetically modified organisms have become a part of our environment and food chain. Roundup Ready soybean is at the moment the most frequent one that man can meet. National regulations require careful monitoring and detection of GMOs. We present in this investigation comparison of several protocols and individual steps, which are included in the whole detection procedure. Currently used CTAB based protocol is suitable for DNA isolation from the green plant tissue but also from the flour. Lectin coding sequence specific primers were suitable for soybean DNA detection unlike leu-tRNA gene specific primers. Only one of the tested CaMV sequence specific primer pairs did not amplify unspecific products under condition tested. NOS terminator specific primer pair was found suitable for routine screening. EPSPS gene was detected using two different protocols; the same primers did not identify other Roundup Ready GM crops.https://cjgpb.agriculturejournals.cz/artkey/cjg-200201-0007_comparison-of-different-pcr-based-protocols-for-detection-of-roundup-ready-soybean.phpgmoroundup ready soybeanpcrdetection |
| spellingShingle | J. Ovesná L. Dedičová J. Horáček E. Sadilová L. Kučera L. Měsková Comparison of different PCR-based protocols for detection of Roundup Ready soybean Czech Journal of Genetics and Plant Breeding gmo roundup ready soybean pcr detection |
| title | Comparison of different PCR-based protocols for detection of Roundup Ready soybean |
| title_full | Comparison of different PCR-based protocols for detection of Roundup Ready soybean |
| title_fullStr | Comparison of different PCR-based protocols for detection of Roundup Ready soybean |
| title_full_unstemmed | Comparison of different PCR-based protocols for detection of Roundup Ready soybean |
| title_short | Comparison of different PCR-based protocols for detection of Roundup Ready soybean |
| title_sort | comparison of different pcr based protocols for detection of roundup ready soybean |
| topic | gmo roundup ready soybean pcr detection |
| url | https://cjgpb.agriculturejournals.cz/artkey/cjg-200201-0007_comparison-of-different-pcr-based-protocols-for-detection-of-roundup-ready-soybean.php |
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