Evaluation of Tissue Expression and Salivary Levels of HER2/neu in Patients with Head and Neck Squamous cell Carcinoma
Introduction: HER2/neu, a member of the epidermal growth factor receptor family, has been shown to be over-expressed in some tumors. The purpose of this study was to determine the salivary levels and tissue expression of HER2/neu in patients with head and neck squamous cell carcinoma (HNSCC) and the...
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| Format: | Article |
| Language: | English |
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Mashhad University of Medical Sciences
2012-10-01
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| Series: | Iranian Journal of Otorhinolaryngology |
| Subjects: | |
| Online Access: | http://ijorl.mums.ac.ir/pdf_668_b706dce7bea8b8f23e9327611db6360d.html |
| _version_ | 1819127290596950016 |
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| author | Soheil Pardis Yasaman Sardari Mohammad Javad Ashraf Azadeh Andisheh Tadbir Hooman Ebrahimi Sara Purshahidi Bijan Khademi Mohammad Javad Fattahi Marzieh Hamzavi |
| author_facet | Soheil Pardis Yasaman Sardari Mohammad Javad Ashraf Azadeh Andisheh Tadbir Hooman Ebrahimi Sara Purshahidi Bijan Khademi Mohammad Javad Fattahi Marzieh Hamzavi |
| author_sort | Soheil Pardis |
| collection | DOAJ |
| description | Introduction:
HER2/neu, a member of the epidermal growth factor receptor family, has been shown to be over-expressed in some tumors. The purpose of this study was to determine the salivary levels and tissue expression of HER2/neu in patients with head and neck squamous cell carcinoma (HNSCC) and their correlation with clinicopathologic parameters.
Materials and Methods:
Enzyme-linked immunosorbent assays (ELISA) were used to evaluate the salivary levels of HER2/neu and immunohistochemistry was used to measure tissue expression of HER2/neu in 28 patients with HNSCC and 25 healthy control subjects.
Results:
The salivary levels of HER2/neu in patients with HNSCC were not significantly higher compared to healthy control subjects. There was no apparent correlation between salivary HER2/neu levels and clinicopathological features such as age, sex, tumor grade, tumor size and nodal status. All HNSCC specimens were positive (membranous or/and cytoplasmic) for HER2/neu, except one sample. Only one HNSCC specimen showed staining purely in the tumor-cell cytoplasm. All control specimens were also positive for both membranous and cytoplasmic HER2/neu but there was a significant difference between the level of cytoplasmic staining in the HNSCC specimens and in the control specimens (P |
| first_indexed | 2024-12-22T08:09:35Z |
| format | Article |
| id | doaj.art-f20ea7dc50a144dca6bd7893fae76b2a |
| institution | Directory Open Access Journal |
| issn | 2251-7251 2251-726X |
| language | English |
| last_indexed | 2024-12-22T08:09:35Z |
| publishDate | 2012-10-01 |
| publisher | Mashhad University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Otorhinolaryngology |
| spelling | doaj.art-f20ea7dc50a144dca6bd7893fae76b2a2022-12-21T18:33:04ZengMashhad University of Medical SciencesIranian Journal of Otorhinolaryngology2251-72512251-726X2012-10-012469161170668Evaluation of Tissue Expression and Salivary Levels of HER2/neu in Patients with Head and Neck Squamous cell CarcinomaSoheil Pardis0Yasaman Sardari1Mohammad Javad Ashraf2Azadeh Andisheh Tadbir3Hooman Ebrahimi4Sara Purshahidi5Bijan Khademi6Mohammad Javad Fattahi7Marzieh Hamzavi8Department of Oral and MaxillofacialPathology, School of Dentistry, Shiraz University of Medical Sciences, Shiraz, Iran.Department of Oral and MaxillofacialPathology, School of Dentistry, Shiraz University of Medical Sciences, Shiraz, Iran.Department of Pathology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.Department of Oral and MaxillofacialPathology, School of Dentistry, Shiraz University of Medical Sciences, Shiraz, Iran.Department of Oral Medicine, School of Dentistry, Shiraz University of Medical Sciences, Shiraz, Iran.Department of Otolaryngology, Khalili Hospital, Shiraz Institute for Cancer Research, Shiraz University of Medical Sciences, Shiraz, Iran.Department of Otolaryngology, Khalili Hospital, Shiraz Institute for Cancer Research, Shiraz University of Medical Sciences, Shiraz, Iran.Shiraz Institute for Cancer Research, Shiraz University of Medical Sciences, Shiraz, Iran.Department of Pathology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.Introduction: HER2/neu, a member of the epidermal growth factor receptor family, has been shown to be over-expressed in some tumors. The purpose of this study was to determine the salivary levels and tissue expression of HER2/neu in patients with head and neck squamous cell carcinoma (HNSCC) and their correlation with clinicopathologic parameters. Materials and Methods: Enzyme-linked immunosorbent assays (ELISA) were used to evaluate the salivary levels of HER2/neu and immunohistochemistry was used to measure tissue expression of HER2/neu in 28 patients with HNSCC and 25 healthy control subjects. Results: The salivary levels of HER2/neu in patients with HNSCC were not significantly higher compared to healthy control subjects. There was no apparent correlation between salivary HER2/neu levels and clinicopathological features such as age, sex, tumor grade, tumor size and nodal status. All HNSCC specimens were positive (membranous or/and cytoplasmic) for HER2/neu, except one sample. Only one HNSCC specimen showed staining purely in the tumor-cell cytoplasm. All control specimens were also positive for both membranous and cytoplasmic HER2/neu but there was a significant difference between the level of cytoplasmic staining in the HNSCC specimens and in the control specimens (Phttp://ijorl.mums.ac.ir/pdf_668_b706dce7bea8b8f23e9327611db6360d.htmlCarcinomaHER2/neuSalivary GlandSquamous cell of head and neckTissue expression |
| spellingShingle | Soheil Pardis Yasaman Sardari Mohammad Javad Ashraf Azadeh Andisheh Tadbir Hooman Ebrahimi Sara Purshahidi Bijan Khademi Mohammad Javad Fattahi Marzieh Hamzavi Evaluation of Tissue Expression and Salivary Levels of HER2/neu in Patients with Head and Neck Squamous cell Carcinoma Iranian Journal of Otorhinolaryngology Carcinoma HER2/neu Salivary Gland Squamous cell of head and neck Tissue expression |
| title | Evaluation of Tissue Expression and Salivary Levels of HER2/neu in Patients with Head and Neck Squamous cell Carcinoma |
| title_full | Evaluation of Tissue Expression and Salivary Levels of HER2/neu in Patients with Head and Neck Squamous cell Carcinoma |
| title_fullStr | Evaluation of Tissue Expression and Salivary Levels of HER2/neu in Patients with Head and Neck Squamous cell Carcinoma |
| title_full_unstemmed | Evaluation of Tissue Expression and Salivary Levels of HER2/neu in Patients with Head and Neck Squamous cell Carcinoma |
| title_short | Evaluation of Tissue Expression and Salivary Levels of HER2/neu in Patients with Head and Neck Squamous cell Carcinoma |
| title_sort | evaluation of tissue expression and salivary levels of her2 neu in patients with head and neck squamous cell carcinoma |
| topic | Carcinoma HER2/neu Salivary Gland Squamous cell of head and neck Tissue expression |
| url | http://ijorl.mums.ac.ir/pdf_668_b706dce7bea8b8f23e9327611db6360d.html |
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