The Cytoskeleton and Its Roles in Self-Organization Phenomena: Insights from <i>Xenopus</i> Egg Extracts
Self-organization of and by the cytoskeleton is central to the biology of the cell. Since their introduction in the early 1980s, cytoplasmic extracts derived from the eggs of the African clawed-frog, <i>Xenopus laevis</i>, have flourished as a major experimental system to study the vario...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-08-01
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| Series: | Cells |
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| Online Access: | https://www.mdpi.com/2073-4409/10/9/2197 |
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| author | Zachary M. Geisterfer Gabriel Guilloux Jesse C. Gatlin Romain Gibeaux |
| author_facet | Zachary M. Geisterfer Gabriel Guilloux Jesse C. Gatlin Romain Gibeaux |
| author_sort | Zachary M. Geisterfer |
| collection | DOAJ |
| description | Self-organization of and by the cytoskeleton is central to the biology of the cell. Since their introduction in the early 1980s, cytoplasmic extracts derived from the eggs of the African clawed-frog, <i>Xenopus laevis</i>, have flourished as a major experimental system to study the various facets of cytoskeleton-dependent self-organization. Over the years, the many investigations that have used these extracts uniquely benefited from their simplified cell cycle, large experimental volumes, biochemical tractability and cell-free nature. Here, we review the contributions of egg extracts to our understanding of the cytoplasmic aspects of self-organization by the microtubule and the actomyosin cytoskeletons as well as the importance of cytoskeletal filaments in organizing nuclear structure and function. |
| first_indexed | 2024-03-10T07:49:52Z |
| format | Article |
| id | doaj.art-f21256339d0d4df2a93b5258d637a9c5 |
| institution | Directory Open Access Journal |
| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-03-10T07:49:52Z |
| publishDate | 2021-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cells |
| spelling | doaj.art-f21256339d0d4df2a93b5258d637a9c52023-11-22T12:22:23ZengMDPI AGCells2073-44092021-08-01109219710.3390/cells10092197The Cytoskeleton and Its Roles in Self-Organization Phenomena: Insights from <i>Xenopus</i> Egg ExtractsZachary M. Geisterfer0Gabriel Guilloux1Jesse C. Gatlin2Romain Gibeaux3Department of Molecular Biology, University of Wyoming, 1000 E. University Ave, Laramie, WY 82070, USAUniv Rennes, CNRS, IGDR (Institut de Génétique et Développement de Rennes)-UMR 6290, F-35000 Rennes, FranceDepartment of Molecular Biology, University of Wyoming, 1000 E. University Ave, Laramie, WY 82070, USAUniv Rennes, CNRS, IGDR (Institut de Génétique et Développement de Rennes)-UMR 6290, F-35000 Rennes, FranceSelf-organization of and by the cytoskeleton is central to the biology of the cell. Since their introduction in the early 1980s, cytoplasmic extracts derived from the eggs of the African clawed-frog, <i>Xenopus laevis</i>, have flourished as a major experimental system to study the various facets of cytoskeleton-dependent self-organization. Over the years, the many investigations that have used these extracts uniquely benefited from their simplified cell cycle, large experimental volumes, biochemical tractability and cell-free nature. Here, we review the contributions of egg extracts to our understanding of the cytoplasmic aspects of self-organization by the microtubule and the actomyosin cytoskeletons as well as the importance of cytoskeletal filaments in organizing nuclear structure and function.https://www.mdpi.com/2073-4409/10/9/2197cytoskeleton<i>Xenopus</i> egg extractself-organizationmicrotubuleactin |
| spellingShingle | Zachary M. Geisterfer Gabriel Guilloux Jesse C. Gatlin Romain Gibeaux The Cytoskeleton and Its Roles in Self-Organization Phenomena: Insights from <i>Xenopus</i> Egg Extracts Cells cytoskeleton <i>Xenopus</i> egg extract self-organization microtubule actin |
| title | The Cytoskeleton and Its Roles in Self-Organization Phenomena: Insights from <i>Xenopus</i> Egg Extracts |
| title_full | The Cytoskeleton and Its Roles in Self-Organization Phenomena: Insights from <i>Xenopus</i> Egg Extracts |
| title_fullStr | The Cytoskeleton and Its Roles in Self-Organization Phenomena: Insights from <i>Xenopus</i> Egg Extracts |
| title_full_unstemmed | The Cytoskeleton and Its Roles in Self-Organization Phenomena: Insights from <i>Xenopus</i> Egg Extracts |
| title_short | The Cytoskeleton and Its Roles in Self-Organization Phenomena: Insights from <i>Xenopus</i> Egg Extracts |
| title_sort | cytoskeleton and its roles in self organization phenomena insights from i xenopus i egg extracts |
| topic | cytoskeleton <i>Xenopus</i> egg extract self-organization microtubule actin |
| url | https://www.mdpi.com/2073-4409/10/9/2197 |
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