Roles of apoptosis and autophagy in natural rabies infections
The aim of this study was to investigate the activity of apoptosis and autophagy in animals (cows, horses, donkeys, dogs and cats) naturally infected with rabies by using immunohistochemistry, immunofluorescence, and qPCR. The mRNA transcript levels of caspase-3, Bax, Bcl2 and LC3B were determined w...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Czech Academy of Agricultural Sciences
2022-01-01
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| Series: | Veterinární Medicína |
| Subjects: | |
| Online Access: | https://vetmed.agriculturejournals.cz/artkey/vet-202201-0001_roles-of-apoptosis-and-autophagy-in-natural-rabies-infections.php |
| _version_ | 1828006530376531968 |
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| author | M Ozkaraca S Ozdemir S Comakli Timurkan MO |
| author_facet | M Ozkaraca S Ozdemir S Comakli Timurkan MO |
| author_sort | M Ozkaraca |
| collection | DOAJ |
| description | The aim of this study was to investigate the activity of apoptosis and autophagy in animals (cows, horses, donkeys, dogs and cats) naturally infected with rabies by using immunohistochemistry, immunofluorescence, and qPCR. The mRNA transcript levels of caspase-3, Bax, Bcl2 and LC3B were determined with qPCR. Caspase-3 and AIF immunopositivity were not observed in the immunohistochemical and immunofluorescence staining, whereas LC3B immunopositivity was determined intensively in the infected animals compared to the control groups. LC3B immunopositivity was detected in the cytoplasm of the Purkinje cells in the cerebellum of the cows, horses and donkeys, and also in the cytoplasm of the neurons in the cornu ammonis of the dogs and cats. While the expression levels of caspase-3 and Bax were downregulated, the Bcl2 expression was up-regulated in the infected animals compared to the uninfected animals. In addition, the LC3B levels were found to be significantly higher in the infected animals. To the best of our knowledge, this work represents the first report of neuronal death in the central nervous system by autophagy, rather than by caspase-dependent or AIF-containing caspase-independent apoptosis. |
| first_indexed | 2024-04-10T07:58:06Z |
| format | Article |
| id | doaj.art-f213fbaac0e141c096b04c850abdd8fe |
| institution | Directory Open Access Journal |
| issn | 0375-8427 1805-9392 |
| language | English |
| last_indexed | 2024-04-10T07:58:06Z |
| publishDate | 2022-01-01 |
| publisher | Czech Academy of Agricultural Sciences |
| record_format | Article |
| series | Veterinární Medicína |
| spelling | doaj.art-f213fbaac0e141c096b04c850abdd8fe2023-02-23T03:51:11ZengCzech Academy of Agricultural SciencesVeterinární Medicína0375-84271805-93922022-01-0167111210.17221/221/2020-VETMEDvet-202201-0001Roles of apoptosis and autophagy in natural rabies infectionsM Ozkaraca0S Ozdemir1S Comakli2Timurkan MO3Department of Pathology, Faculty of Veterinary Medicine, Cumhuriyet University, Sivas, TurkeyDepartment of Genetic, Faculty of Veterinary Medicine, Ataturk University, Erzurum, TurkeyDepartment of Pathology, Faculty of Veterinary Medicine, Ataturk University, Erzurum, TurkeyDepartment of Virology, Faculty of Veterinary Medicine, Ataturk University, Erzurum, TurkeyThe aim of this study was to investigate the activity of apoptosis and autophagy in animals (cows, horses, donkeys, dogs and cats) naturally infected with rabies by using immunohistochemistry, immunofluorescence, and qPCR. The mRNA transcript levels of caspase-3, Bax, Bcl2 and LC3B were determined with qPCR. Caspase-3 and AIF immunopositivity were not observed in the immunohistochemical and immunofluorescence staining, whereas LC3B immunopositivity was determined intensively in the infected animals compared to the control groups. LC3B immunopositivity was detected in the cytoplasm of the Purkinje cells in the cerebellum of the cows, horses and donkeys, and also in the cytoplasm of the neurons in the cornu ammonis of the dogs and cats. While the expression levels of caspase-3 and Bax were downregulated, the Bcl2 expression was up-regulated in the infected animals compared to the uninfected animals. In addition, the LC3B levels were found to be significantly higher in the infected animals. To the best of our knowledge, this work represents the first report of neuronal death in the central nervous system by autophagy, rather than by caspase-dependent or AIF-containing caspase-independent apoptosis.https://vetmed.agriculturejournals.cz/artkey/vet-202201-0001_roles-of-apoptosis-and-autophagy-in-natural-rabies-infections.phpaifcaspase-3lc3b |
| spellingShingle | M Ozkaraca S Ozdemir S Comakli Timurkan MO Roles of apoptosis and autophagy in natural rabies infections Veterinární Medicína aif caspase-3 lc3b |
| title | Roles of apoptosis and autophagy in natural rabies infections |
| title_full | Roles of apoptosis and autophagy in natural rabies infections |
| title_fullStr | Roles of apoptosis and autophagy in natural rabies infections |
| title_full_unstemmed | Roles of apoptosis and autophagy in natural rabies infections |
| title_short | Roles of apoptosis and autophagy in natural rabies infections |
| title_sort | roles of apoptosis and autophagy in natural rabies infections |
| topic | aif caspase-3 lc3b |
| url | https://vetmed.agriculturejournals.cz/artkey/vet-202201-0001_roles-of-apoptosis-and-autophagy-in-natural-rabies-infections.php |
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