A multimodal analytical method to simultaneously determine monoacetyldiacylglycerols, medium and long chain triglycerides in biological samples during routine lipidomics
Abstract Background Monoacetyldiglycerides (MAcDG), are acetylated triglycerides (TG) and an emerging class of bioactive or functional lipid with promising nutritional, medical, and industrial applications. A major challenge exists when analyzing MAcDG from other subclasses of TG in biological matri...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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BMC
2022-05-01
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| Series: | Lipids in Health and Disease |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12944-022-01650-w |
| _version_ | 1818202190524710912 |
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| author | Charles F. Manful Thu H. Pham Heather Spicer Raymond H. Thomas |
| author_facet | Charles F. Manful Thu H. Pham Heather Spicer Raymond H. Thomas |
| author_sort | Charles F. Manful |
| collection | DOAJ |
| description | Abstract Background Monoacetyldiglycerides (MAcDG), are acetylated triglycerides (TG) and an emerging class of bioactive or functional lipid with promising nutritional, medical, and industrial applications. A major challenge exists when analyzing MAcDG from other subclasses of TG in biological matrices, limiting knowledge on their applications and metabolism. Methods Herein a multimodal analytical method for resolution, identification, and quantitation of MAcDG in biological samples was demonstrated based on thin layer chromatography-flame ionization detection complimentary with C30-reversed phase liquid chromatography-high resolution accurate mass tandem mass spectrometry. This method was then applied to determine the MAcDG molecular species composition and quantity in E. solidaginis larvae. The statistical method for analysis of TG subclass composition and molecular species composition of E. solidaginis larvae was one-way analysis of variance (ANOVA). Results The findings suggest that the proposed analytical method could simultaneously provide a fast, accurate, sensitive, high throughput analysis of MAcDG from other TG subclasses, including the fatty acids, isomers, and molecular species composition. Conclusion This method would allow for MAcDG to be included during routine lipidomics analysis of biological samples and will have broad interests and applications in the scientific community in areas such as nutrition, climate change, medicine and biofuel innovations. Graphical abstract |
| first_indexed | 2024-12-12T03:05:31Z |
| format | Article |
| id | doaj.art-f2169029e2264e9a81c789999fc064cd |
| institution | Directory Open Access Journal |
| issn | 1476-511X |
| language | English |
| last_indexed | 2024-12-12T03:05:31Z |
| publishDate | 2022-05-01 |
| publisher | BMC |
| record_format | Article |
| series | Lipids in Health and Disease |
| spelling | doaj.art-f2169029e2264e9a81c789999fc064cd2022-12-22T00:40:32ZengBMCLipids in Health and Disease1476-511X2022-05-0121111710.1186/s12944-022-01650-wA multimodal analytical method to simultaneously determine monoacetyldiacylglycerols, medium and long chain triglycerides in biological samples during routine lipidomicsCharles F. Manful0Thu H. Pham1Heather Spicer2Raymond H. Thomas3School of Science and the Environment/ Boreal Ecosystem Research Initiative, Grenfell Campus, Memorial University of NewfoundlandSchool of Science and the Environment/ Boreal Ecosystem Research Initiative, Grenfell Campus, Memorial University of NewfoundlandSchool of Science and the Environment/ Boreal Ecosystem Research Initiative, Grenfell Campus, Memorial University of NewfoundlandSchool of Science and the Environment/ Boreal Ecosystem Research Initiative, Grenfell Campus, Memorial University of NewfoundlandAbstract Background Monoacetyldiglycerides (MAcDG), are acetylated triglycerides (TG) and an emerging class of bioactive or functional lipid with promising nutritional, medical, and industrial applications. A major challenge exists when analyzing MAcDG from other subclasses of TG in biological matrices, limiting knowledge on their applications and metabolism. Methods Herein a multimodal analytical method for resolution, identification, and quantitation of MAcDG in biological samples was demonstrated based on thin layer chromatography-flame ionization detection complimentary with C30-reversed phase liquid chromatography-high resolution accurate mass tandem mass spectrometry. This method was then applied to determine the MAcDG molecular species composition and quantity in E. solidaginis larvae. The statistical method for analysis of TG subclass composition and molecular species composition of E. solidaginis larvae was one-way analysis of variance (ANOVA). Results The findings suggest that the proposed analytical method could simultaneously provide a fast, accurate, sensitive, high throughput analysis of MAcDG from other TG subclasses, including the fatty acids, isomers, and molecular species composition. Conclusion This method would allow for MAcDG to be included during routine lipidomics analysis of biological samples and will have broad interests and applications in the scientific community in areas such as nutrition, climate change, medicine and biofuel innovations. Graphical abstracthttps://doi.org/10.1186/s12944-022-01650-wThin layer chromatographyTriglyceride analysisAcylated triglycerideLiquid chromatographyMass spectrometryFunctional lipids |
| spellingShingle | Charles F. Manful Thu H. Pham Heather Spicer Raymond H. Thomas A multimodal analytical method to simultaneously determine monoacetyldiacylglycerols, medium and long chain triglycerides in biological samples during routine lipidomics Lipids in Health and Disease Thin layer chromatography Triglyceride analysis Acylated triglyceride Liquid chromatography Mass spectrometry Functional lipids |
| title | A multimodal analytical method to simultaneously determine monoacetyldiacylglycerols, medium and long chain triglycerides in biological samples during routine lipidomics |
| title_full | A multimodal analytical method to simultaneously determine monoacetyldiacylglycerols, medium and long chain triglycerides in biological samples during routine lipidomics |
| title_fullStr | A multimodal analytical method to simultaneously determine monoacetyldiacylglycerols, medium and long chain triglycerides in biological samples during routine lipidomics |
| title_full_unstemmed | A multimodal analytical method to simultaneously determine monoacetyldiacylglycerols, medium and long chain triglycerides in biological samples during routine lipidomics |
| title_short | A multimodal analytical method to simultaneously determine monoacetyldiacylglycerols, medium and long chain triglycerides in biological samples during routine lipidomics |
| title_sort | multimodal analytical method to simultaneously determine monoacetyldiacylglycerols medium and long chain triglycerides in biological samples during routine lipidomics |
| topic | Thin layer chromatography Triglyceride analysis Acylated triglyceride Liquid chromatography Mass spectrometry Functional lipids |
| url | https://doi.org/10.1186/s12944-022-01650-w |
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