Fractionation of Soil Organic Matter into Labile and Stable Fractions

The present study aims to test and evaluate the efficiency of a new modified method of organic matter evaluation. It allows the assessment of the quality and quantity of the primary soil organic matter and the stable organic fractions separately. The new method was tested in six soil samples of diff...

Full description

Bibliographic Details
Main Authors: Marek Kopecký, Ladislav Kolář, Kristýna Perná, Radka Váchalová, Petr Mráz, Petr Konvalina, Yves Theoneste Murindangabo, Mohammad Ghorbani, Ladislav Menšík, Miroslav Dumbrovský
Format: Article
Language:English
Published: MDPI AG 2021-12-01
Series:Agronomy
Subjects:
Online Access:https://www.mdpi.com/2073-4395/12/1/73
_version_ 1827667323520024576
author Marek Kopecký
Ladislav Kolář
Kristýna Perná
Radka Váchalová
Petr Mráz
Petr Konvalina
Yves Theoneste Murindangabo
Mohammad Ghorbani
Ladislav Menšík
Miroslav Dumbrovský
author_facet Marek Kopecký
Ladislav Kolář
Kristýna Perná
Radka Váchalová
Petr Mráz
Petr Konvalina
Yves Theoneste Murindangabo
Mohammad Ghorbani
Ladislav Menšík
Miroslav Dumbrovský
author_sort Marek Kopecký
collection DOAJ
description The present study aims to test and evaluate the efficiency of a new modified method of organic matter evaluation. It allows the assessment of the quality and quantity of the primary soil organic matter and the stable organic fractions separately. The new method was tested in six soil samples of different localities in the Czech Republic. This method is based on observing reaction kinetics during the oxidation of soil organic matter and measuring the cation-exchange capacity of stable organic fractions. The results were compared with classical methods, which rely on the isolation of humic substances, determination of the content of humic acids and fulvic acids and their ratio C<sub>HA</sub>:C<sub>FA</sub>, quotient E<sub>4/6</sub>, and fractionation of soil organic matter according to resistance to oxidation. It turned out that the results of the new modified method are more sensitive in comparison with the results obtained by classical procedures. The linear regression demonstrated the dependence between the amounts of soil organic matter determined by the classical method compared with the modified method. Moreover, the new modified method was found to be faster and not demanding on laboratory equipment. The new method has been improved to be easily repeatable, and some shortcomings of the previous method were eliminated. Based on our results and other recent studies, the modified method may be recommended for the practical evaluation of soil organic matter conditions.
first_indexed 2024-03-10T03:08:05Z
format Article
id doaj.art-f21b5d26482d4b46bc183c10845939a9
institution Directory Open Access Journal
issn 2073-4395
language English
last_indexed 2024-03-10T03:08:05Z
publishDate 2021-12-01
publisher MDPI AG
record_format Article
series Agronomy
spelling doaj.art-f21b5d26482d4b46bc183c10845939a92023-11-23T12:37:48ZengMDPI AGAgronomy2073-43952021-12-011217310.3390/agronomy12010073Fractionation of Soil Organic Matter into Labile and Stable FractionsMarek Kopecký0Ladislav Kolář1Kristýna Perná2Radka Váchalová3Petr Mráz4Petr Konvalina5Yves Theoneste Murindangabo6Mohammad Ghorbani7Ladislav Menšík8Miroslav Dumbrovský9Faculty of Agriculture, University of South Bohemia in Ceske Budejovice, 370 05 Ceske Budejovice, Czech RepublicFaculty of Agriculture, University of South Bohemia in Ceske Budejovice, 370 05 Ceske Budejovice, Czech RepublicFaculty of Agriculture, University of South Bohemia in Ceske Budejovice, 370 05 Ceske Budejovice, Czech RepublicFaculty of Agriculture, University of South Bohemia in Ceske Budejovice, 370 05 Ceske Budejovice, Czech RepublicFaculty of Agriculture, University of South Bohemia in Ceske Budejovice, 370 05 Ceske Budejovice, Czech RepublicFaculty of Agriculture, University of South Bohemia in Ceske Budejovice, 370 05 Ceske Budejovice, Czech RepublicFaculty of Agriculture, University of South Bohemia in Ceske Budejovice, 370 05 Ceske Budejovice, Czech RepublicFaculty of Agriculture, University of South Bohemia in Ceske Budejovice, 370 05 Ceske Budejovice, Czech RepublicDivision of Crop Management Systems, Crop Research Institute, 161 06 Prague, Czech RepublicFaculty of Civil Engineering, Brno University of Technology, 602 00 Brno, Czech RepublicThe present study aims to test and evaluate the efficiency of a new modified method of organic matter evaluation. It allows the assessment of the quality and quantity of the primary soil organic matter and the stable organic fractions separately. The new method was tested in six soil samples of different localities in the Czech Republic. This method is based on observing reaction kinetics during the oxidation of soil organic matter and measuring the cation-exchange capacity of stable organic fractions. The results were compared with classical methods, which rely on the isolation of humic substances, determination of the content of humic acids and fulvic acids and their ratio C<sub>HA</sub>:C<sub>FA</sub>, quotient E<sub>4/6</sub>, and fractionation of soil organic matter according to resistance to oxidation. It turned out that the results of the new modified method are more sensitive in comparison with the results obtained by classical procedures. The linear regression demonstrated the dependence between the amounts of soil organic matter determined by the classical method compared with the modified method. Moreover, the new modified method was found to be faster and not demanding on laboratory equipment. The new method has been improved to be easily repeatable, and some shortcomings of the previous method were eliminated. Based on our results and other recent studies, the modified method may be recommended for the practical evaluation of soil organic matter conditions.https://www.mdpi.com/2073-4395/12/1/73analytical methodsfractionationlabilitymodified methodsoil organic matterstability
spellingShingle Marek Kopecký
Ladislav Kolář
Kristýna Perná
Radka Váchalová
Petr Mráz
Petr Konvalina
Yves Theoneste Murindangabo
Mohammad Ghorbani
Ladislav Menšík
Miroslav Dumbrovský
Fractionation of Soil Organic Matter into Labile and Stable Fractions
Agronomy
analytical methods
fractionation
lability
modified method
soil organic matter
stability
title Fractionation of Soil Organic Matter into Labile and Stable Fractions
title_full Fractionation of Soil Organic Matter into Labile and Stable Fractions
title_fullStr Fractionation of Soil Organic Matter into Labile and Stable Fractions
title_full_unstemmed Fractionation of Soil Organic Matter into Labile and Stable Fractions
title_short Fractionation of Soil Organic Matter into Labile and Stable Fractions
title_sort fractionation of soil organic matter into labile and stable fractions
topic analytical methods
fractionation
lability
modified method
soil organic matter
stability
url https://www.mdpi.com/2073-4395/12/1/73
work_keys_str_mv AT marekkopecky fractionationofsoilorganicmatterintolabileandstablefractions
AT ladislavkolar fractionationofsoilorganicmatterintolabileandstablefractions
AT kristynaperna fractionationofsoilorganicmatterintolabileandstablefractions
AT radkavachalova fractionationofsoilorganicmatterintolabileandstablefractions
AT petrmraz fractionationofsoilorganicmatterintolabileandstablefractions
AT petrkonvalina fractionationofsoilorganicmatterintolabileandstablefractions
AT yvestheonestemurindangabo fractionationofsoilorganicmatterintolabileandstablefractions
AT mohammadghorbani fractionationofsoilorganicmatterintolabileandstablefractions
AT ladislavmensik fractionationofsoilorganicmatterintolabileandstablefractions
AT miroslavdumbrovsky fractionationofsoilorganicmatterintolabileandstablefractions