Effect of various media and supplements on laccase activity and its application in dyes decolorization
Aims: Marine-derived fungi are a potential for the search of new compounds with relevant features. Among these, theligninolytic enzymes have potential applications in a large number of fields, including the environmental and industrialsectors. This work aimed to evaluate the laccase activity of the...
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Malaysian Society for Microbiology
2013-01-01
|
| Series: | Malaysian Journal of Microbiology |
| Subjects: | |
| Online Access: | http://web.usm.my/mjm/issues/vol9no2/Research%207.pdf |
| _version_ | 1818552024268013568 |
|---|---|
| author | Abeer, A. A. E. A. Faten, A. M. |
| author_facet | Abeer, A. A. E. A. Faten, A. M. |
| author_sort | Abeer, A. A. E. A. |
| collection | DOAJ |
| description | Aims: Marine-derived fungi are a potential for the search of new compounds with relevant features. Among these, theligninolytic enzymes have potential applications in a large number of fields, including the environmental and industrialsectors. This work aimed to evaluate the laccase activity of the marine-derived fungus Alternaria alternata, undervarious cultivation conditions and its application in synthetic dyes decolorization.Methodology and results: Wheat bran prepared with 40 mL sea water proportion was the most suitable substrate forlaccase production (114.06±2.24 U/mL) by A. alternata, after 14 days of incubation in submerged fermentation. Laccaseproduction in static cultivation was superior to that in agitated cultures. The simple Boyd and Kohlmeyer medium withsupplementation of 2 mM CuSO4·5H2O on day 6, at an incubation period of 14 days and incubation temperature of 28±2°C under static conditions, yielded amounts of laccase (36.13±0.34 U/mL) less than that obtained with submergedfermentation of wheat bran as unique substrate. Furthermore, A. alternata has high decolorization capability toward azodyes in the absence of redox mediators, 75.47% of the reactive black at 0.01% concentration, was removed after 30days of incubation. Also has good ability to decolorize the triphenylmethane dye crystal violet, at 0.01% concentration,about 69.35% of the dye was removed after 30 days.Conclusion, significance and impact of study: These unusual properties demonstrate that the marine-derived fungusAlternaria alternata has potentials in specific industrial or environmental applications. |
| first_indexed | 2024-12-12T09:07:41Z |
| format | Article |
| id | doaj.art-f21c19d36b0746fa89cc296ed8741279 |
| institution | Directory Open Access Journal |
| issn | 1823-8262 2231-7538 |
| language | English |
| last_indexed | 2024-12-12T09:07:41Z |
| publishDate | 2013-01-01 |
| publisher | Malaysian Society for Microbiology |
| record_format | Article |
| series | Malaysian Journal of Microbiology |
| spelling | doaj.art-f21c19d36b0746fa89cc296ed87412792022-12-22T00:29:38ZengMalaysian Society for MicrobiologyMalaysian Journal of Microbiology1823-82622231-75382013-01-0192166175Effect of various media and supplements on laccase activity and its application in dyes decolorizationAbeer, A. A. E. A.Faten, A. M.Aims: Marine-derived fungi are a potential for the search of new compounds with relevant features. Among these, theligninolytic enzymes have potential applications in a large number of fields, including the environmental and industrialsectors. This work aimed to evaluate the laccase activity of the marine-derived fungus Alternaria alternata, undervarious cultivation conditions and its application in synthetic dyes decolorization.Methodology and results: Wheat bran prepared with 40 mL sea water proportion was the most suitable substrate forlaccase production (114.06±2.24 U/mL) by A. alternata, after 14 days of incubation in submerged fermentation. Laccaseproduction in static cultivation was superior to that in agitated cultures. The simple Boyd and Kohlmeyer medium withsupplementation of 2 mM CuSO4·5H2O on day 6, at an incubation period of 14 days and incubation temperature of 28±2°C under static conditions, yielded amounts of laccase (36.13±0.34 U/mL) less than that obtained with submergedfermentation of wheat bran as unique substrate. Furthermore, A. alternata has high decolorization capability toward azodyes in the absence of redox mediators, 75.47% of the reactive black at 0.01% concentration, was removed after 30days of incubation. Also has good ability to decolorize the triphenylmethane dye crystal violet, at 0.01% concentration,about 69.35% of the dye was removed after 30 days.Conclusion, significance and impact of study: These unusual properties demonstrate that the marine-derived fungusAlternaria alternata has potentials in specific industrial or environmental applications.http://web.usm.my/mjm/issues/vol9no2/Research%207.pdfMarine-derived fungiLaccaseAgricultural wastesInducersDecolorization |
| spellingShingle | Abeer, A. A. E. A. Faten, A. M. Effect of various media and supplements on laccase activity and its application in dyes decolorization Malaysian Journal of Microbiology Marine-derived fungi Laccase Agricultural wastes Inducers Decolorization |
| title | Effect of various media and supplements on laccase activity and its application in dyes decolorization |
| title_full | Effect of various media and supplements on laccase activity and its application in dyes decolorization |
| title_fullStr | Effect of various media and supplements on laccase activity and its application in dyes decolorization |
| title_full_unstemmed | Effect of various media and supplements on laccase activity and its application in dyes decolorization |
| title_short | Effect of various media and supplements on laccase activity and its application in dyes decolorization |
| title_sort | effect of various media and supplements on laccase activity and its application in dyes decolorization |
| topic | Marine-derived fungi Laccase Agricultural wastes Inducers Decolorization |
| url | http://web.usm.my/mjm/issues/vol9no2/Research%207.pdf |
| work_keys_str_mv | AT abeeraaea effectofvariousmediaandsupplementsonlaccaseactivityanditsapplicationindyesdecolorization AT fatenam effectofvariousmediaandsupplementsonlaccaseactivityanditsapplicationindyesdecolorization |