Lactobacillus rhamnosus GG ameliorates osteoporosis in ovariectomized rats by regulating the Th17/Treg balance and gut microbiota structure
ABSTRACTBackground With increasing knowledge about the gut – bone axis, more studies for treatments based on the regulation of postmenopausal osteoporosis by gut microbes are being conducted. Based on our previous work, this study was conducted to further investigate the therapeutic effects of Lacto...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2023-12-01
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| Series: | Gut Microbes |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/19490976.2023.2190304 |
| _version_ | 1797236689048961024 |
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| author | Mengyu Guo Huanjin Liu Yinting Yu Xingyu Zhu Hui Xie Chenxu Wei Chunmei Mei Yun Shi Nong Zhou Kunming Qin Weidong Li |
| author_facet | Mengyu Guo Huanjin Liu Yinting Yu Xingyu Zhu Hui Xie Chenxu Wei Chunmei Mei Yun Shi Nong Zhou Kunming Qin Weidong Li |
| author_sort | Mengyu Guo |
| collection | DOAJ |
| description | ABSTRACTBackground With increasing knowledge about the gut – bone axis, more studies for treatments based on the regulation of postmenopausal osteoporosis by gut microbes are being conducted. Based on our previous work, this study was conducted to further investigate the therapeutic effects of Lactobacillus rhamnosus GG (LGG) on ovariectomized (OVX) model rats and the immunological and microecological mechanisms involved.Results We found a protective effect of LGG treatment in OVX rats through changes in bone microarchitecture, bone biomechanics, and CTX-I, PINP, Ca, and RANKL expression levels. LGG was more advantageous in promoting osteogenesis, which may be responsible for the alleviation of osteoporosis. Th17 cells were imbalanced with Treg cells in mediastinal lymph nodes and bone marrow, with RORγt and FOXP3 expression following a similar trend. TNF-α and IL-17 expression in colon and bone marrow increased, while TGF-β and IL-10 expression decreased; however, LGG treatment modulated these changes and improved the Th17/Treg balance significantly. Regarding the intestinal barrier, we found that LGG treatment ameliorated estrogen deficiency-induced inflammation and mucosal damage and increased the expression of GLP-2 R and tight junction proteins. Importantly, 16S rRNA sequencing showed a significant increase in the Firmicutes/Bacteroidetes ratio during estrogen deficiency. Dominant intestinal flora showed significant differences in composition; LGG treatment regulated the various genera that were imbalanced in OVX, along with modifying those that did not change significantly in other groups with respect to the intestinal barrier, inflammation development, and bile acid metabolism.Conclusions Overall, LGG ameliorated estrogen deficiency-induced osteoporosis by regulating the gut microbiome and intestinal barrier and stimulating Th17/Treg balance in gut and bone. |
| first_indexed | 2024-03-11T14:19:48Z |
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| institution | Directory Open Access Journal |
| issn | 1949-0976 1949-0984 |
| language | English |
| last_indexed | 2024-04-24T17:07:50Z |
| publishDate | 2023-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Gut Microbes |
| spelling | doaj.art-f21c2983421b4d02b9802390978d27482024-03-28T22:38:20ZengTaylor & Francis GroupGut Microbes1949-09761949-09842023-12-0115110.1080/19490976.2023.2190304Lactobacillus rhamnosus GG ameliorates osteoporosis in ovariectomized rats by regulating the Th17/Treg balance and gut microbiota structureMengyu Guo0Huanjin Liu1Yinting Yu2Xingyu Zhu3Hui Xie4Chenxu Wei5Chunmei Mei6Yun Shi7Nong Zhou8Kunming Qin9Weidong Li10School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, ChinaSchool of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, ChinaSchool of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, ChinaSchool of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, ChinaSchool of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, ChinaSchool of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, ChinaSchool of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, ChinaSchool of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, ChinaSchool of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, ChinaSchool of Pharmacy, Jiangsu Ocean University, Lianyungang, ChinaSchool of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, ChinaABSTRACTBackground With increasing knowledge about the gut – bone axis, more studies for treatments based on the regulation of postmenopausal osteoporosis by gut microbes are being conducted. Based on our previous work, this study was conducted to further investigate the therapeutic effects of Lactobacillus rhamnosus GG (LGG) on ovariectomized (OVX) model rats and the immunological and microecological mechanisms involved.Results We found a protective effect of LGG treatment in OVX rats through changes in bone microarchitecture, bone biomechanics, and CTX-I, PINP, Ca, and RANKL expression levels. LGG was more advantageous in promoting osteogenesis, which may be responsible for the alleviation of osteoporosis. Th17 cells were imbalanced with Treg cells in mediastinal lymph nodes and bone marrow, with RORγt and FOXP3 expression following a similar trend. TNF-α and IL-17 expression in colon and bone marrow increased, while TGF-β and IL-10 expression decreased; however, LGG treatment modulated these changes and improved the Th17/Treg balance significantly. Regarding the intestinal barrier, we found that LGG treatment ameliorated estrogen deficiency-induced inflammation and mucosal damage and increased the expression of GLP-2 R and tight junction proteins. Importantly, 16S rRNA sequencing showed a significant increase in the Firmicutes/Bacteroidetes ratio during estrogen deficiency. Dominant intestinal flora showed significant differences in composition; LGG treatment regulated the various genera that were imbalanced in OVX, along with modifying those that did not change significantly in other groups with respect to the intestinal barrier, inflammation development, and bile acid metabolism.Conclusions Overall, LGG ameliorated estrogen deficiency-induced osteoporosis by regulating the gut microbiome and intestinal barrier and stimulating Th17/Treg balance in gut and bone.https://www.tandfonline.com/doi/10.1080/19490976.2023.2190304OsteoporosisOVXLactobacillus rhamnosusTh17/Treggut microbiotagut barrier |
| spellingShingle | Mengyu Guo Huanjin Liu Yinting Yu Xingyu Zhu Hui Xie Chenxu Wei Chunmei Mei Yun Shi Nong Zhou Kunming Qin Weidong Li Lactobacillus rhamnosus GG ameliorates osteoporosis in ovariectomized rats by regulating the Th17/Treg balance and gut microbiota structure Gut Microbes Osteoporosis OVX Lactobacillus rhamnosus Th17/Treg gut microbiota gut barrier |
| title | Lactobacillus rhamnosus GG ameliorates osteoporosis in ovariectomized rats by regulating the Th17/Treg balance and gut microbiota structure |
| title_full | Lactobacillus rhamnosus GG ameliorates osteoporosis in ovariectomized rats by regulating the Th17/Treg balance and gut microbiota structure |
| title_fullStr | Lactobacillus rhamnosus GG ameliorates osteoporosis in ovariectomized rats by regulating the Th17/Treg balance and gut microbiota structure |
| title_full_unstemmed | Lactobacillus rhamnosus GG ameliorates osteoporosis in ovariectomized rats by regulating the Th17/Treg balance and gut microbiota structure |
| title_short | Lactobacillus rhamnosus GG ameliorates osteoporosis in ovariectomized rats by regulating the Th17/Treg balance and gut microbiota structure |
| title_sort | lactobacillus rhamnosus gg ameliorates osteoporosis in ovariectomized rats by regulating the th17 treg balance and gut microbiota structure |
| topic | Osteoporosis OVX Lactobacillus rhamnosus Th17/Treg gut microbiota gut barrier |
| url | https://www.tandfonline.com/doi/10.1080/19490976.2023.2190304 |
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