Profile of subpopulation composition of regulatory T lymphocytes and intestinal microbiota in patients with irritable bowel syndrome
The following specificcharacteristics of the composition of intestinal microbiota in patients with irritable bowel syndrome (IBS) were identified using a metagenomic analysis (16 S rRNA): 1) an increase in the representation of Actinobacteria, including Bifidobacterium spp., Firmicutes, including re...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | Russian |
| Published: |
St. Petersburg branch of the Russian Association of Allergologists and Clinical Immunologists
2020-04-01
|
| Series: | Медицинская иммунология |
| Subjects: | |
| Online Access: | https://www.mimmun.ru/mimmun/article/view/1905 |
| _version_ | 1797197920120864768 |
|---|---|
| author | E. I. Ermolenko I. V. Kudryavtsev O. I. Solovyova A. B. Karaseva A. N. Tsapieva Yu. D. Kondratenko M. P. Kotyleva M. K. Serebryakova I. A. Shumikhina A. N. Suvorov |
| author_facet | E. I. Ermolenko I. V. Kudryavtsev O. I. Solovyova A. B. Karaseva A. N. Tsapieva Yu. D. Kondratenko M. P. Kotyleva M. K. Serebryakova I. A. Shumikhina A. N. Suvorov |
| author_sort | E. I. Ermolenko |
| collection | DOAJ |
| description | The following specificcharacteristics of the composition of intestinal microbiota in patients with irritable bowel syndrome (IBS) were identified using a metagenomic analysis (16 S rRNA): 1) an increase in the representation of Actinobacteria, including Bifidobacterium spp., Firmicutes, including representatives of Streptococcaceae (Streptococcus), Lachnosperaceae (Dorea), Veillonellaceae (Dialister), Proteobacteria (Enterobacteriaceae and Desulfovibrionaceae families); 2) a decrease in the population of Bacteroidetes, including representatives of the families Prevotellacea (Prevotella spp.), Bacteroidaceae (Bacteroides spp.). Firmicutes belonging to the families Clostridiaceae and Ruminococcaceae (Fecalibacterium spp.).Flow cytometry in the study of the subpopulation composition of T regulatory (Treg) lymphocytes in patients with IBS revealed an increase in the number of CD45R0+CD62L+ central memory cells (CM), which can regulate the processes of maturation and differentiation of lymphocytes in lymphoid tissue. A decrease in the expression of exonucleases CD39 and CD73 was detected, which can have a significant effect on their activity. A reduction in effector memory cells (EM) Treg was observed.Changes in the expression level of exonucleases CD39 and CD73 were inversely correlated with the content of Proteobacteria and the representation of the genera Bifidobacterium spp. and Faecalibacterium spp. The content of СЫ Treg was directly correlated with the content of Dorea spp.The results may be indicative of impairment in the processes of Treg differentiation, which are closely related to changes in key components of intestinal microbiocenosis in IBS. |
| first_indexed | 2024-03-08T05:48:17Z |
| format | Article |
| id | doaj.art-f21cd7d917eb4f0db65244cd22e3c152 |
| institution | Directory Open Access Journal |
| issn | 1563-0625 2313-741X |
| language | Russian |
| last_indexed | 2024-04-24T06:51:37Z |
| publishDate | 2020-04-01 |
| publisher | St. Petersburg branch of the Russian Association of Allergologists and Clinical Immunologists |
| record_format | Article |
| series | Медицинская иммунология |
| spelling | doaj.art-f21cd7d917eb4f0db65244cd22e3c1522024-04-22T13:07:45ZrusSt. Petersburg branch of the Russian Association of Allergologists and Clinical ImmunologistsМедицинская иммунология1563-06252313-741X2020-04-0122233534610.15789/1563-0625-POS-19051266Profile of subpopulation composition of regulatory T lymphocytes and intestinal microbiota in patients with irritable bowel syndromeE. I. Ermolenko0I. V. Kudryavtsev1O. I. Solovyova2A. B. Karaseva3A. N. Tsapieva4Yu. D. Kondratenko5M. P. Kotyleva6M. K. Serebryakova7I. A. Shumikhina8A. N. Suvorov9ФГБНУ Институт экспериментальной медицины; ФГБОУВО Санкт-Петербургский государственный университетInstitute of Experimental Medicine; St. Petersburg State University; First St. Petersburg State I. Pavlov Medical UniversityNorth-Western State I. Mechnikov Medical UniversityInstitute of Experimental MedicineInstitute of Experimental MedicineSt. Petersburg State UniversityInstitute of Experimental MedicineInstitute of Experimental MedicineNorth-Western State I. Mechnikov Medical UniversityInstitute of Experimental Medicine; St. Petersburg State UniversityThe following specificcharacteristics of the composition of intestinal microbiota in patients with irritable bowel syndrome (IBS) were identified using a metagenomic analysis (16 S rRNA): 1) an increase in the representation of Actinobacteria, including Bifidobacterium spp., Firmicutes, including representatives of Streptococcaceae (Streptococcus), Lachnosperaceae (Dorea), Veillonellaceae (Dialister), Proteobacteria (Enterobacteriaceae and Desulfovibrionaceae families); 2) a decrease in the population of Bacteroidetes, including representatives of the families Prevotellacea (Prevotella spp.), Bacteroidaceae (Bacteroides spp.). Firmicutes belonging to the families Clostridiaceae and Ruminococcaceae (Fecalibacterium spp.).Flow cytometry in the study of the subpopulation composition of T regulatory (Treg) lymphocytes in patients with IBS revealed an increase in the number of CD45R0+CD62L+ central memory cells (CM), which can regulate the processes of maturation and differentiation of lymphocytes in lymphoid tissue. A decrease in the expression of exonucleases CD39 and CD73 was detected, which can have a significant effect on their activity. A reduction in effector memory cells (EM) Treg was observed.Changes in the expression level of exonucleases CD39 and CD73 were inversely correlated with the content of Proteobacteria and the representation of the genera Bifidobacterium spp. and Faecalibacterium spp. The content of СЫ Treg was directly correlated with the content of Dorea spp.The results may be indicative of impairment in the processes of Treg differentiation, which are closely related to changes in key components of intestinal microbiocenosis in IBS.https://www.mimmun.ru/mimmun/article/view/1905intestinal microbiocenosismetagenomic analysisflow cytometry of a subpopulation of t lymphocytes |
| spellingShingle | E. I. Ermolenko I. V. Kudryavtsev O. I. Solovyova A. B. Karaseva A. N. Tsapieva Yu. D. Kondratenko M. P. Kotyleva M. K. Serebryakova I. A. Shumikhina A. N. Suvorov Profile of subpopulation composition of regulatory T lymphocytes and intestinal microbiota in patients with irritable bowel syndrome Медицинская иммунология intestinal microbiocenosis metagenomic analysis flow cytometry of a subpopulation of t lymphocytes |
| title | Profile of subpopulation composition of regulatory T lymphocytes and intestinal microbiota in patients with irritable bowel syndrome |
| title_full | Profile of subpopulation composition of regulatory T lymphocytes and intestinal microbiota in patients with irritable bowel syndrome |
| title_fullStr | Profile of subpopulation composition of regulatory T lymphocytes and intestinal microbiota in patients with irritable bowel syndrome |
| title_full_unstemmed | Profile of subpopulation composition of regulatory T lymphocytes and intestinal microbiota in patients with irritable bowel syndrome |
| title_short | Profile of subpopulation composition of regulatory T lymphocytes and intestinal microbiota in patients with irritable bowel syndrome |
| title_sort | profile of subpopulation composition of regulatory t lymphocytes and intestinal microbiota in patients with irritable bowel syndrome |
| topic | intestinal microbiocenosis metagenomic analysis flow cytometry of a subpopulation of t lymphocytes |
| url | https://www.mimmun.ru/mimmun/article/view/1905 |
| work_keys_str_mv | AT eiermolenko profileofsubpopulationcompositionofregulatorytlymphocytesandintestinalmicrobiotainpatientswithirritablebowelsyndrome AT ivkudryavtsev profileofsubpopulationcompositionofregulatorytlymphocytesandintestinalmicrobiotainpatientswithirritablebowelsyndrome AT oisolovyova profileofsubpopulationcompositionofregulatorytlymphocytesandintestinalmicrobiotainpatientswithirritablebowelsyndrome AT abkaraseva profileofsubpopulationcompositionofregulatorytlymphocytesandintestinalmicrobiotainpatientswithirritablebowelsyndrome AT antsapieva profileofsubpopulationcompositionofregulatorytlymphocytesandintestinalmicrobiotainpatientswithirritablebowelsyndrome AT yudkondratenko profileofsubpopulationcompositionofregulatorytlymphocytesandintestinalmicrobiotainpatientswithirritablebowelsyndrome AT mpkotyleva profileofsubpopulationcompositionofregulatorytlymphocytesandintestinalmicrobiotainpatientswithirritablebowelsyndrome AT mkserebryakova profileofsubpopulationcompositionofregulatorytlymphocytesandintestinalmicrobiotainpatientswithirritablebowelsyndrome AT iashumikhina profileofsubpopulationcompositionofregulatorytlymphocytesandintestinalmicrobiotainpatientswithirritablebowelsyndrome AT ansuvorov profileofsubpopulationcompositionofregulatorytlymphocytesandintestinalmicrobiotainpatientswithirritablebowelsyndrome |