X-ray Fluorescence Techniques in Determining the Habitat Preferences of Species—<i>Ulva pilifera</i> (Ulvales, Chlorophyta) from Montenegro Case Study
The paper presents four new sites where bright green <i>Ulva</i> thalli were found inhabiting freshwater (a river, a ditch, the Milet Canal) and marine (on the rocky shore of the Adriatic Sea) habitats in Montenegro. The aims of this study were to determine, for the first time, whether s...
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MDPI AG
2020-10-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/25/21/5022 |
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| author | Joanna Czerwik-Marcinkowska Renata Piwowarczyk Bohuslav Uher Ewa Tomal Anna Wojciechowska |
| author_facet | Joanna Czerwik-Marcinkowska Renata Piwowarczyk Bohuslav Uher Ewa Tomal Anna Wojciechowska |
| author_sort | Joanna Czerwik-Marcinkowska |
| collection | DOAJ |
| description | The paper presents four new sites where bright green <i>Ulva</i> thalli were found inhabiting freshwater (a river, a ditch, the Milet Canal) and marine (on the rocky shore of the Adriatic Sea) habitats in Montenegro. The aims of this study were to determine, for the first time, whether specimens of <i>Ulva pilifera</i> collected in Montenegro are phylogenetically and morphologically the same species as the one occurring in Europe. Using total reflection X-ray fluorescence (TXRF) and wavelength dispersive X-ray fluorescence (WDXRF) techniques it assessed the elemental composition of their thalli and its influence to colonise new habitats. Elements: Al, As, Ba, Br, Ca, Cl, Cr, Cu, Fe, Hf, I, K, Mg, Mn, Na, Ni, P, Pb, Rb, S, Si, Sr, Ti, V, and Zn were determined. The highest elemental concentrations were found for Ca = 16.3% (using WDXRF) and for Sr = 292 ppm (using TXRF) in the <i>Ulva</i> thalli. <i>Ulva pilifera</i> analysed from Montenegro, based on classical morphological methods and molecular techniques, are closely related to the same species from inland and coastal waters throughout Europe. The analysis of trace elements showed that the metal content in <i>Ulva</i> thalli is correlated with the trace elements in water and sediments. <i>Ulva pilifera</i> fits numerous features that make it one of the bioindicators of marine pollution, thanks to its worldwide distribution and capacity to accumulate trace elements. |
| first_indexed | 2024-03-10T15:14:24Z |
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| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T15:14:24Z |
| publishDate | 2020-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-f21de23b8d784a709e90eb4be82d504f2023-11-20T19:04:49ZengMDPI AGMolecules1420-30492020-10-012521502210.3390/molecules25215022X-ray Fluorescence Techniques in Determining the Habitat Preferences of Species—<i>Ulva pilifera</i> (Ulvales, Chlorophyta) from Montenegro Case StudyJoanna Czerwik-Marcinkowska0Renata Piwowarczyk1Bohuslav Uher2Ewa Tomal3Anna Wojciechowska4Institute of Biology, Jan Kochanowski University, 25-406 Kielce, PolandInstitute of Biology, Jan Kochanowski University, 25-406 Kielce, PolandDepartment of Microbiology, University of Vienna, 1010 Vienna, AustriaProvincial Sanitary and Epidemiological Station, 25-734 Kielce, PolandDepartment of Geobotany and Landscape Planning, Nicolaus Copernicus University, 87-100 Toruń, PolandThe paper presents four new sites where bright green <i>Ulva</i> thalli were found inhabiting freshwater (a river, a ditch, the Milet Canal) and marine (on the rocky shore of the Adriatic Sea) habitats in Montenegro. The aims of this study were to determine, for the first time, whether specimens of <i>Ulva pilifera</i> collected in Montenegro are phylogenetically and morphologically the same species as the one occurring in Europe. Using total reflection X-ray fluorescence (TXRF) and wavelength dispersive X-ray fluorescence (WDXRF) techniques it assessed the elemental composition of their thalli and its influence to colonise new habitats. Elements: Al, As, Ba, Br, Ca, Cl, Cr, Cu, Fe, Hf, I, K, Mg, Mn, Na, Ni, P, Pb, Rb, S, Si, Sr, Ti, V, and Zn were determined. The highest elemental concentrations were found for Ca = 16.3% (using WDXRF) and for Sr = 292 ppm (using TXRF) in the <i>Ulva</i> thalli. <i>Ulva pilifera</i> analysed from Montenegro, based on classical morphological methods and molecular techniques, are closely related to the same species from inland and coastal waters throughout Europe. The analysis of trace elements showed that the metal content in <i>Ulva</i> thalli is correlated with the trace elements in water and sediments. <i>Ulva pilifera</i> fits numerous features that make it one of the bioindicators of marine pollution, thanks to its worldwide distribution and capacity to accumulate trace elements.https://www.mdpi.com/1420-3049/25/21/5022elemental compositionmacroalgaeUlvalesWDXRFTXRF |
| spellingShingle | Joanna Czerwik-Marcinkowska Renata Piwowarczyk Bohuslav Uher Ewa Tomal Anna Wojciechowska X-ray Fluorescence Techniques in Determining the Habitat Preferences of Species—<i>Ulva pilifera</i> (Ulvales, Chlorophyta) from Montenegro Case Study Molecules elemental composition macroalgae Ulvales WDXRF TXRF |
| title | X-ray Fluorescence Techniques in Determining the Habitat Preferences of Species—<i>Ulva pilifera</i> (Ulvales, Chlorophyta) from Montenegro Case Study |
| title_full | X-ray Fluorescence Techniques in Determining the Habitat Preferences of Species—<i>Ulva pilifera</i> (Ulvales, Chlorophyta) from Montenegro Case Study |
| title_fullStr | X-ray Fluorescence Techniques in Determining the Habitat Preferences of Species—<i>Ulva pilifera</i> (Ulvales, Chlorophyta) from Montenegro Case Study |
| title_full_unstemmed | X-ray Fluorescence Techniques in Determining the Habitat Preferences of Species—<i>Ulva pilifera</i> (Ulvales, Chlorophyta) from Montenegro Case Study |
| title_short | X-ray Fluorescence Techniques in Determining the Habitat Preferences of Species—<i>Ulva pilifera</i> (Ulvales, Chlorophyta) from Montenegro Case Study |
| title_sort | x ray fluorescence techniques in determining the habitat preferences of species i ulva pilifera i ulvales chlorophyta from montenegro case study |
| topic | elemental composition macroalgae Ulvales WDXRF TXRF |
| url | https://www.mdpi.com/1420-3049/25/21/5022 |
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