Fabrication of Blood Capillary Models for Live Imaging Microarray Analysis
Conventional microarray analysis usually deals with the monolayer or two-dimensional (2D) assays for the high-throughput screening applications. Even though these cell-based assays are effective for preliminary screening at least to have information on cytotoxicity, they do not adequately re-create...
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| Format: | Article |
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MDPI AG
2020-07-01
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| Series: | Micromachines |
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| Online Access: | https://www.mdpi.com/2072-666X/11/8/727 |
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| author | Muhammad Asri Abdul Sisak Fiona Louis Sun Hyeok Lee Young-Tae Chang Michiya Matsusaki |
| author_facet | Muhammad Asri Abdul Sisak Fiona Louis Sun Hyeok Lee Young-Tae Chang Michiya Matsusaki |
| author_sort | Muhammad Asri Abdul Sisak |
| collection | DOAJ |
| description | Conventional microarray analysis usually deals with the monolayer or two-dimensional (2D) assays for the high-throughput screening applications. Even though these cell-based assays are effective for preliminary screening at least to have information on cytotoxicity, they do not adequately re-create the in vivo complexity of three-dimensional (3D) tissues. In this study, 3D-blood capillary models were constructed by using physiological collagen microfibers (CMF), which provide the extracellular matrix in the complex tissue. Micro-droplets of fibrin gels containing CMF, endothelial cells, and fibroblasts were cultured for five days in 48-wells plate to provide a medium-throughput system for screening applications. Blood capillaries networks were formed by optimizing the concentration of CMF used and the number of cells. Finally, this screening method was a powerful assay for the application on the selection of not only a specific chemical probe for blood capillary live-imaging, but also a drug, aptamer, and peptide with potential blood vessel targeting property. |
| first_indexed | 2024-03-10T18:11:35Z |
| format | Article |
| id | doaj.art-f223561bb4f34c5e848f0635c8d68d17 |
| institution | Directory Open Access Journal |
| issn | 2072-666X |
| language | English |
| last_indexed | 2024-03-10T18:11:35Z |
| publishDate | 2020-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Micromachines |
| spelling | doaj.art-f223561bb4f34c5e848f0635c8d68d172023-11-20T08:06:31ZengMDPI AGMicromachines2072-666X2020-07-0111872710.3390/mi11080727Fabrication of Blood Capillary Models for Live Imaging Microarray AnalysisMuhammad Asri Abdul Sisak0Fiona Louis1Sun Hyeok Lee2Young-Tae Chang3Michiya Matsusaki4Department of Applied Chemistry, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, JapanJoint Research Laboratory (TOPPAN) for Advanced Cell Regulatory Chemistry, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, JapanCenter for Self-assembly and Complexity, Institute for Basic Science (IBS), Pohang 37673, KoreaCenter for Self-assembly and Complexity, Institute for Basic Science (IBS), Pohang 37673, KoreaDepartment of Applied Chemistry, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, JapanConventional microarray analysis usually deals with the monolayer or two-dimensional (2D) assays for the high-throughput screening applications. Even though these cell-based assays are effective for preliminary screening at least to have information on cytotoxicity, they do not adequately re-create the in vivo complexity of three-dimensional (3D) tissues. In this study, 3D-blood capillary models were constructed by using physiological collagen microfibers (CMF), which provide the extracellular matrix in the complex tissue. Micro-droplets of fibrin gels containing CMF, endothelial cells, and fibroblasts were cultured for five days in 48-wells plate to provide a medium-throughput system for screening applications. Blood capillaries networks were formed by optimizing the concentration of CMF used and the number of cells. Finally, this screening method was a powerful assay for the application on the selection of not only a specific chemical probe for blood capillary live-imaging, but also a drug, aptamer, and peptide with potential blood vessel targeting property.https://www.mdpi.com/2072-666X/11/8/727blood capillary modelsfibrin gelscollagen microfibers |
| spellingShingle | Muhammad Asri Abdul Sisak Fiona Louis Sun Hyeok Lee Young-Tae Chang Michiya Matsusaki Fabrication of Blood Capillary Models for Live Imaging Microarray Analysis Micromachines blood capillary models fibrin gels collagen microfibers |
| title | Fabrication of Blood Capillary Models for Live Imaging Microarray Analysis |
| title_full | Fabrication of Blood Capillary Models for Live Imaging Microarray Analysis |
| title_fullStr | Fabrication of Blood Capillary Models for Live Imaging Microarray Analysis |
| title_full_unstemmed | Fabrication of Blood Capillary Models for Live Imaging Microarray Analysis |
| title_short | Fabrication of Blood Capillary Models for Live Imaging Microarray Analysis |
| title_sort | fabrication of blood capillary models for live imaging microarray analysis |
| topic | blood capillary models fibrin gels collagen microfibers |
| url | https://www.mdpi.com/2072-666X/11/8/727 |
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