Identification of drought tolerant mechanisms in a drought-tolerant maize mutant based on physiological, biochemical and transcriptomic analyses
Abstract Background Frequently occurring drought stress negatively affects the production of maize worldwide. Numerous efforts have been made to develop drought-tolerant maize lines and to explore drought tolerant mechanisms in maize. However, there is a lack of comparative studies on transcriptomic...
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| Format: | Article |
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BMC
2020-07-01
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| Series: | BMC Plant Biology |
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| Online Access: | http://link.springer.com/article/10.1186/s12870-020-02526-w |
| _version_ | 1818952675916513280 |
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| author | Qinbin Zhang Hui Liu Xiaolin Wu Wei Wang |
| author_facet | Qinbin Zhang Hui Liu Xiaolin Wu Wei Wang |
| author_sort | Qinbin Zhang |
| collection | DOAJ |
| description | Abstract Background Frequently occurring drought stress negatively affects the production of maize worldwide. Numerous efforts have been made to develop drought-tolerant maize lines and to explore drought tolerant mechanisms in maize. However, there is a lack of comparative studies on transcriptomic changes between drought-tolerant and control maize lines. Results In the present study, we have developed a drought-tolerant maize mutant (C7–2t) by irradiating the seeds of maize inbred line ChangC7–2 (C7–2) with 60Co-γ. Compared to its wild type C7–2, C7–2t exhibited a significantly delayed wilting and higher drought tolerance under both the controlled and field conditions, indicating its high water-holding ability. Transcriptomic profiling was performed to identify differentially expressed genes (DEGs) between C7–2 and C7–2t during drought. As a result, a total of 4552 DEGs were implied in drought tolerance of C7-2 and C7-2t. In particular, the expression of photosynthesis-related genes in C7–2 was inhibited, whereas these genes in C7–2t were almost unaffected under drought. Moreover, a specific set of the DEGs were involved in phenylpropanoid biosynthesis and taurine (hypotaurine) metabolism in C7–2t; these DEGs were enriched in cell components associated with membrane systems and cell wall biosynthesis. Conclusions The drought tolerance of C7–2t was largely due to its high water-holding ability, stable photosynthesis (for supporting osmoregulation) and strengthened biosynthesis of cell walls under drought conditions. |
| first_indexed | 2024-12-20T09:54:09Z |
| format | Article |
| id | doaj.art-f224ad9aed9c4ce08c523b5005355951 |
| institution | Directory Open Access Journal |
| issn | 1471-2229 |
| language | English |
| last_indexed | 2024-12-20T09:54:09Z |
| publishDate | 2020-07-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Plant Biology |
| spelling | doaj.art-f224ad9aed9c4ce08c523b50053559512022-12-21T19:44:30ZengBMCBMC Plant Biology1471-22292020-07-0120111410.1186/s12870-020-02526-wIdentification of drought tolerant mechanisms in a drought-tolerant maize mutant based on physiological, biochemical and transcriptomic analysesQinbin Zhang0Hui Liu1Xiaolin Wu2Wei Wang3College of Life Sciences, National Key Laboratory of Wheat and Maize Crop Science, Henan Agricultural UniversityCollege of Life Sciences, National Key Laboratory of Wheat and Maize Crop Science, Henan Agricultural UniversityCollege of Life Sciences, National Key Laboratory of Wheat and Maize Crop Science, Henan Agricultural UniversityCollege of Life Sciences, National Key Laboratory of Wheat and Maize Crop Science, Henan Agricultural UniversityAbstract Background Frequently occurring drought stress negatively affects the production of maize worldwide. Numerous efforts have been made to develop drought-tolerant maize lines and to explore drought tolerant mechanisms in maize. However, there is a lack of comparative studies on transcriptomic changes between drought-tolerant and control maize lines. Results In the present study, we have developed a drought-tolerant maize mutant (C7–2t) by irradiating the seeds of maize inbred line ChangC7–2 (C7–2) with 60Co-γ. Compared to its wild type C7–2, C7–2t exhibited a significantly delayed wilting and higher drought tolerance under both the controlled and field conditions, indicating its high water-holding ability. Transcriptomic profiling was performed to identify differentially expressed genes (DEGs) between C7–2 and C7–2t during drought. As a result, a total of 4552 DEGs were implied in drought tolerance of C7-2 and C7-2t. In particular, the expression of photosynthesis-related genes in C7–2 was inhibited, whereas these genes in C7–2t were almost unaffected under drought. Moreover, a specific set of the DEGs were involved in phenylpropanoid biosynthesis and taurine (hypotaurine) metabolism in C7–2t; these DEGs were enriched in cell components associated with membrane systems and cell wall biosynthesis. Conclusions The drought tolerance of C7–2t was largely due to its high water-holding ability, stable photosynthesis (for supporting osmoregulation) and strengthened biosynthesis of cell walls under drought conditions.http://link.springer.com/article/10.1186/s12870-020-02526-wDifferentially expressed genesDrought-adaptation modelDrought-tolerant mutantDrought stressTranscriptomic analysisZea mays |
| spellingShingle | Qinbin Zhang Hui Liu Xiaolin Wu Wei Wang Identification of drought tolerant mechanisms in a drought-tolerant maize mutant based on physiological, biochemical and transcriptomic analyses BMC Plant Biology Differentially expressed genes Drought-adaptation model Drought-tolerant mutant Drought stress Transcriptomic analysis Zea mays |
| title | Identification of drought tolerant mechanisms in a drought-tolerant maize mutant based on physiological, biochemical and transcriptomic analyses |
| title_full | Identification of drought tolerant mechanisms in a drought-tolerant maize mutant based on physiological, biochemical and transcriptomic analyses |
| title_fullStr | Identification of drought tolerant mechanisms in a drought-tolerant maize mutant based on physiological, biochemical and transcriptomic analyses |
| title_full_unstemmed | Identification of drought tolerant mechanisms in a drought-tolerant maize mutant based on physiological, biochemical and transcriptomic analyses |
| title_short | Identification of drought tolerant mechanisms in a drought-tolerant maize mutant based on physiological, biochemical and transcriptomic analyses |
| title_sort | identification of drought tolerant mechanisms in a drought tolerant maize mutant based on physiological biochemical and transcriptomic analyses |
| topic | Differentially expressed genes Drought-adaptation model Drought-tolerant mutant Drought stress Transcriptomic analysis Zea mays |
| url | http://link.springer.com/article/10.1186/s12870-020-02526-w |
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