Widespread bacterial responses and their mechanism of bacterial metallogenic detoxification under high concentrations of heavy metals
Microbial mineralization is increasingly used in bioremediation of heavy metal pollution, but better mechanistic understanding of the processes involved and how they are regulated are required to improve the practical application of microorganisms in bioremediation. We used a combination of morpholo...
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| Format: | Article |
| Language: | English |
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Elsevier
2022-11-01
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| Series: | Ecotoxicology and Environmental Safety |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0147651322010338 |
| _version_ | 1811256306808389632 |
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| author | Xiaofang Li Menglin Sun Luting Zhang Roger D. Finlay Renlu Liu Bin Lian |
| author_facet | Xiaofang Li Menglin Sun Luting Zhang Roger D. Finlay Renlu Liu Bin Lian |
| author_sort | Xiaofang Li |
| collection | DOAJ |
| description | Microbial mineralization is increasingly used in bioremediation of heavy metal pollution, but better mechanistic understanding of the processes involved and how they are regulated are required to improve the practical application of microorganisms in bioremediation. We used a combination of morphological (TEM) and analytical (XRD, XPS, FTIR) methods, together with novel proteomic analyses, to investigate the detoxification mechanisms, used by a range of bacteria, including the strains Bacillus velezensis LB002, Escherichia coli DH5α, B. subtilis 168, Pseudomonas putida KT2440, and B. licheniformis MT-1, exposed to elevated concentrations of Cd2+ and combinations of Cd2+, Pb2+, Cu2+, and Zn2+, in the presence and absence of added CaCl2. Common features of detoxification included biomineralization, including the production of biological vaterite, up-regulation of proteins involved in flagellar movement and chemotaxis, biofilm synthesis, transmembrane transport of small molecules and organic matter decomposition. The putative roles of differentially expressed proteins in detoxification are discussed in relation to chemical and morphological data and together provide important tools to improve screening, selection, and practical application of bacterial isolates in bioremediation of polluted environments. |
| first_indexed | 2024-04-12T17:38:09Z |
| format | Article |
| id | doaj.art-f225b8295144417c8b25ff9971641d57 |
| institution | Directory Open Access Journal |
| issn | 0147-6513 |
| language | English |
| last_indexed | 2024-04-12T17:38:09Z |
| publishDate | 2022-11-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Ecotoxicology and Environmental Safety |
| spelling | doaj.art-f225b8295144417c8b25ff9971641d572022-12-22T03:22:54ZengElsevierEcotoxicology and Environmental Safety0147-65132022-11-01246114193Widespread bacterial responses and their mechanism of bacterial metallogenic detoxification under high concentrations of heavy metalsXiaofang Li0Menglin Sun1Luting Zhang2Roger D. Finlay3Renlu Liu4Bin Lian5Jiangsu Key Laboratory for Microbes and Functional Genomics, College of Life Sciences, Nanjing Normal University, Nanjing 210023, ChinaJiangsu Key Laboratory for Microbes and Functional Genomics, College of Life Sciences, Nanjing Normal University, Nanjing 210023, ChinaJiangsu Key Laboratory for Microbes and Functional Genomics, College of Life Sciences, Nanjing Normal University, Nanjing 210023, ChinaDepartment of Forest Mycology and Plant Pathology, Uppsala BioCenter, Swedish University of Agricultural Sciences, 75007, Uppsala, SwedenJiangsu Key Laboratory for Microbes and Functional Genomics, College of Life Sciences, Nanjing Normal University, Nanjing 210023, China; School of Life Sciences, Key Laboratory of Agricultural Environmental Pollution Prevention and Control in Red Soil Hilly Region of Jiangxi Province, Jinggangshan University, Ji'an 343009, ChinaCollege of Marine Science and Engineering, Nanjing Normal University, Nanjing 210023, China; Corresponding author.Microbial mineralization is increasingly used in bioremediation of heavy metal pollution, but better mechanistic understanding of the processes involved and how they are regulated are required to improve the practical application of microorganisms in bioremediation. We used a combination of morphological (TEM) and analytical (XRD, XPS, FTIR) methods, together with novel proteomic analyses, to investigate the detoxification mechanisms, used by a range of bacteria, including the strains Bacillus velezensis LB002, Escherichia coli DH5α, B. subtilis 168, Pseudomonas putida KT2440, and B. licheniformis MT-1, exposed to elevated concentrations of Cd2+ and combinations of Cd2+, Pb2+, Cu2+, and Zn2+, in the presence and absence of added CaCl2. Common features of detoxification included biomineralization, including the production of biological vaterite, up-regulation of proteins involved in flagellar movement and chemotaxis, biofilm synthesis, transmembrane transport of small molecules and organic matter decomposition. The putative roles of differentially expressed proteins in detoxification are discussed in relation to chemical and morphological data and together provide important tools to improve screening, selection, and practical application of bacterial isolates in bioremediation of polluted environments.http://www.sciencedirect.com/science/article/pii/S0147651322010338Metallogenic detoxificationHeavy metalMicrobial remediationProteomicsMolecular mechanism |
| spellingShingle | Xiaofang Li Menglin Sun Luting Zhang Roger D. Finlay Renlu Liu Bin Lian Widespread bacterial responses and their mechanism of bacterial metallogenic detoxification under high concentrations of heavy metals Ecotoxicology and Environmental Safety Metallogenic detoxification Heavy metal Microbial remediation Proteomics Molecular mechanism |
| title | Widespread bacterial responses and their mechanism of bacterial metallogenic detoxification under high concentrations of heavy metals |
| title_full | Widespread bacterial responses and their mechanism of bacterial metallogenic detoxification under high concentrations of heavy metals |
| title_fullStr | Widespread bacterial responses and their mechanism of bacterial metallogenic detoxification under high concentrations of heavy metals |
| title_full_unstemmed | Widespread bacterial responses and their mechanism of bacterial metallogenic detoxification under high concentrations of heavy metals |
| title_short | Widespread bacterial responses and their mechanism of bacterial metallogenic detoxification under high concentrations of heavy metals |
| title_sort | widespread bacterial responses and their mechanism of bacterial metallogenic detoxification under high concentrations of heavy metals |
| topic | Metallogenic detoxification Heavy metal Microbial remediation Proteomics Molecular mechanism |
| url | http://www.sciencedirect.com/science/article/pii/S0147651322010338 |
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