A new transient expression system for large-scale production of recombinant proteins in plants based on air-brushing an Agrobacterium suspension
Plant transient expression using virus-based vectors is advantageous when high level of gene expression is desired within a short time. In this study, a new system, named “air-brush,” has been developed to facilitate a scale-up production of recombinant proteins in plants. GFP was expressed successf...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2015-06-01
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| Series: | Biotechnology Reports |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2215017X15000077 |
| _version_ | 1818959362550398976 |
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| author | Taicheng Jin Jing Wang Xiaojuan Zhu Yanan Xu Xiaofu Zhou Liping Yang |
| author_facet | Taicheng Jin Jing Wang Xiaojuan Zhu Yanan Xu Xiaofu Zhou Liping Yang |
| author_sort | Taicheng Jin |
| collection | DOAJ |
| description | Plant transient expression using virus-based vectors is advantageous when high level of gene expression is desired within a short time. In this study, a new system, named “air-brush,” has been developed to facilitate a scale-up production of recombinant proteins in plants. GFP was expressed successfully in Nicotiana benthamiana (Nb) plants by air-brushing an Agrobacterium suspension that contained the TMV-based vector p35S-30B-GFP. Key factors influencing the gene expression were optimized, including the Agrobacterium cell density, seedling age, and the growth temperature of plant materials. In addition, the pharmaceutical protein human acidic fibroblast growth factor (ha FGF) was also expressed in Nb plants by the air-brush system. The results demonstrated that using this system is highly advantageous; it is convenient, quick, easily scaled-up, and has a higher expression efficiency than leaf infiltration. |
| first_indexed | 2024-12-20T11:40:26Z |
| format | Article |
| id | doaj.art-f225f978916b4945a381080b6a4aacf1 |
| institution | Directory Open Access Journal |
| issn | 2215-017X |
| language | English |
| last_indexed | 2024-12-20T11:40:26Z |
| publishDate | 2015-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Biotechnology Reports |
| spelling | doaj.art-f225f978916b4945a381080b6a4aacf12022-12-21T19:42:01ZengElsevierBiotechnology Reports2215-017X2015-06-016C364010.1016/j.btre.2015.01.004A new transient expression system for large-scale production of recombinant proteins in plants based on air-brushing an Agrobacterium suspensionTaicheng Jin0Jing Wang1Xiaojuan Zhu2Yanan Xu3Xiaofu Zhou4Liping Yang5The School of Life Sciences, Jilin Normal University, Siping 136000, ChinaThe School of Life Sciences, Jilin Normal University, Siping 136000, ChinaThe Institute of Genetics and Cytology, Northeast Normal University, Changchun 130024, ChinaThe School of Life Sciences, Jilin Normal University, Siping 136000, ChinaThe School of Life Sciences, Jilin Normal University, Siping 136000, ChinaThe School of Life Sciences, Jilin Normal University, Siping 136000, ChinaPlant transient expression using virus-based vectors is advantageous when high level of gene expression is desired within a short time. In this study, a new system, named “air-brush,” has been developed to facilitate a scale-up production of recombinant proteins in plants. GFP was expressed successfully in Nicotiana benthamiana (Nb) plants by air-brushing an Agrobacterium suspension that contained the TMV-based vector p35S-30B-GFP. Key factors influencing the gene expression were optimized, including the Agrobacterium cell density, seedling age, and the growth temperature of plant materials. In addition, the pharmaceutical protein human acidic fibroblast growth factor (ha FGF) was also expressed in Nb plants by the air-brush system. The results demonstrated that using this system is highly advantageous; it is convenient, quick, easily scaled-up, and has a higher expression efficiency than leaf infiltration.http://www.sciencedirect.com/science/article/pii/S2215017X15000077Air-brush systemAgroinoculationTobacco mosaic virus (TMV)GFP |
| spellingShingle | Taicheng Jin Jing Wang Xiaojuan Zhu Yanan Xu Xiaofu Zhou Liping Yang A new transient expression system for large-scale production of recombinant proteins in plants based on air-brushing an Agrobacterium suspension Biotechnology Reports Air-brush system Agroinoculation Tobacco mosaic virus (TMV) GFP |
| title | A new transient expression system for large-scale production of recombinant proteins in plants based on air-brushing an Agrobacterium suspension |
| title_full | A new transient expression system for large-scale production of recombinant proteins in plants based on air-brushing an Agrobacterium suspension |
| title_fullStr | A new transient expression system for large-scale production of recombinant proteins in plants based on air-brushing an Agrobacterium suspension |
| title_full_unstemmed | A new transient expression system for large-scale production of recombinant proteins in plants based on air-brushing an Agrobacterium suspension |
| title_short | A new transient expression system for large-scale production of recombinant proteins in plants based on air-brushing an Agrobacterium suspension |
| title_sort | new transient expression system for large scale production of recombinant proteins in plants based on air brushing an agrobacterium suspension |
| topic | Air-brush system Agroinoculation Tobacco mosaic virus (TMV) GFP |
| url | http://www.sciencedirect.com/science/article/pii/S2215017X15000077 |
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