Phytocyanin-encoding genes confer enhanced ozone tolerance in Arabidopsis thaliana
Abstract Ozone is a phytotoxic air pollutant that has various damaging effects on plants, including chlorosis and growth inhibition. Although various physiological and genetic studies have elucidated some of the mechanisms underlying plant ozone sensitivity and lesion development, our understanding...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2022-12-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-022-25706-0 |
| _version_ | 1797977577832316928 |
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| author | Shoko Saji Hikaru Saji Kimiyo Sage-Ono Michiyuki Ono Nobuyoshi Nakajima Mitsuko Aono |
| author_facet | Shoko Saji Hikaru Saji Kimiyo Sage-Ono Michiyuki Ono Nobuyoshi Nakajima Mitsuko Aono |
| author_sort | Shoko Saji |
| collection | DOAJ |
| description | Abstract Ozone is a phytotoxic air pollutant that has various damaging effects on plants, including chlorosis and growth inhibition. Although various physiological and genetic studies have elucidated some of the mechanisms underlying plant ozone sensitivity and lesion development, our understanding of plant response to this gas remains incomplete. Here, we show evidence for the involvement of certain apoplastic proteins called phytocyanins, such as AtUC5, that protect against ozone damage. Two representative ozone-inducible responses, chlorosis and stomatal closure, were suppressed in AtUC5-overexpressing plants. Analysis of transgenic plants expressing a chimeric protein composed of AtUC5 fused to green fluorescent protein indicated that this fusion protein localises to the apoplast of plant cells where it appears to suppress early responses to ozone damage such as generation or signalling of reactive oxygen species. Moreover, yeast two-hybrid analyses suggest that AtUC5 may physically interact with stress-related proteins such as copper amine oxidase and late embryogenesis abundant protein-like protein. In addition to AtUC5, other examined phytocyanins such as AtUC6 and AtSC3 could confer ozone tolerance to plants when overexpressed in A. thaliana, suggesting that these proteins act together to protect plants against oxidative stress factors. |
| first_indexed | 2024-04-11T05:09:10Z |
| format | Article |
| id | doaj.art-f22760284bfd458e933dde6b9082e9cb |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-11T05:09:10Z |
| publishDate | 2022-12-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-f22760284bfd458e933dde6b9082e9cb2022-12-25T12:10:43ZengNature PortfolioScientific Reports2045-23222022-12-0112111110.1038/s41598-022-25706-0Phytocyanin-encoding genes confer enhanced ozone tolerance in Arabidopsis thalianaShoko Saji0Hikaru Saji1Kimiyo Sage-Ono2Michiyuki Ono3Nobuyoshi Nakajima4Mitsuko Aono5Biodiversity Division, National Institute for Environmental StudiesBiodiversity Division, National Institute for Environmental StudiesGraduate School of Life and Environmental Science, University of TsukubaGraduate School of Life and Environmental Science, University of TsukubaBiodiversity Division, National Institute for Environmental StudiesBiodiversity Division, National Institute for Environmental StudiesAbstract Ozone is a phytotoxic air pollutant that has various damaging effects on plants, including chlorosis and growth inhibition. Although various physiological and genetic studies have elucidated some of the mechanisms underlying plant ozone sensitivity and lesion development, our understanding of plant response to this gas remains incomplete. Here, we show evidence for the involvement of certain apoplastic proteins called phytocyanins, such as AtUC5, that protect against ozone damage. Two representative ozone-inducible responses, chlorosis and stomatal closure, were suppressed in AtUC5-overexpressing plants. Analysis of transgenic plants expressing a chimeric protein composed of AtUC5 fused to green fluorescent protein indicated that this fusion protein localises to the apoplast of plant cells where it appears to suppress early responses to ozone damage such as generation or signalling of reactive oxygen species. Moreover, yeast two-hybrid analyses suggest that AtUC5 may physically interact with stress-related proteins such as copper amine oxidase and late embryogenesis abundant protein-like protein. In addition to AtUC5, other examined phytocyanins such as AtUC6 and AtSC3 could confer ozone tolerance to plants when overexpressed in A. thaliana, suggesting that these proteins act together to protect plants against oxidative stress factors.https://doi.org/10.1038/s41598-022-25706-0 |
| spellingShingle | Shoko Saji Hikaru Saji Kimiyo Sage-Ono Michiyuki Ono Nobuyoshi Nakajima Mitsuko Aono Phytocyanin-encoding genes confer enhanced ozone tolerance in Arabidopsis thaliana Scientific Reports |
| title | Phytocyanin-encoding genes confer enhanced ozone tolerance in Arabidopsis thaliana |
| title_full | Phytocyanin-encoding genes confer enhanced ozone tolerance in Arabidopsis thaliana |
| title_fullStr | Phytocyanin-encoding genes confer enhanced ozone tolerance in Arabidopsis thaliana |
| title_full_unstemmed | Phytocyanin-encoding genes confer enhanced ozone tolerance in Arabidopsis thaliana |
| title_short | Phytocyanin-encoding genes confer enhanced ozone tolerance in Arabidopsis thaliana |
| title_sort | phytocyanin encoding genes confer enhanced ozone tolerance in arabidopsis thaliana |
| url | https://doi.org/10.1038/s41598-022-25706-0 |
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