Molecular Diagnosis of Mycoplasma pneumoniae in Synovial Fluid of Rheumatoid Arthritis Patients
Background and Aim: The role of Mycoplasma super antigens in inflammatory diseases such as rheumatoid arthritis (RA) is taken into consideration. Therefore, the aim of this study was to diagnosis of Mycoplasma pneumoniae in synovial fluid (SF) of patients with RA.Materials and Methods: In this study...
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| Format: | Article |
| Language: | English |
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Farname
2014-06-01
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| Series: | Iranian Journal of Medical Microbiology |
| Subjects: | |
| Online Access: | http://www.ijmm.ir/browse.php?a_code=A-10-69-1&slc_lang=en&sid=1 |
| _version_ | 1818913314534588416 |
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| author | Reza Golmohammadi Ramezan Ali Ataee Gholam Hossein Alishiri Reza Mirnejad Ali Najafi Mehdi Tate Davoude Esmaieli Nematollah Jonaidi Jafari |
| author_facet | Reza Golmohammadi Ramezan Ali Ataee Gholam Hossein Alishiri Reza Mirnejad Ali Najafi Mehdi Tate Davoude Esmaieli Nematollah Jonaidi Jafari |
| author_sort | Reza Golmohammadi |
| collection | DOAJ |
| description | Background and Aim: The role of Mycoplasma super antigens in inflammatory diseases such as rheumatoid arthritis (RA) is taken into consideration. Therefore, the aim of this study was to diagnosis of Mycoplasma pneumoniae in synovial fluid (SF) of patients with RA.Materials and Methods: In this study, 131 synovial fluid samples of patients with RA were collected during 3 years (2011- 2013). Mycoplasma pneumoniae
ATCC: 29342 strain was used as control. The genome of samples is extracted separately and PCR protocol by M. pneumoniae species and mycoplasma genus identifier primers was carried out. The PCR product was confirmed by sequencing. Results: The results of this study showed that this molecular diagnosis method is able to detect M. pneumoniae in the synovial fluid of patients with RA. So that M. pneumoniae species was confirmed in 30 samples (22.9%) and Mycoplasma genus detected in the 70 sample (53.4%). Conclusions: based on the results of this study, the PCR molecular method was able to detect of the Mycoplasma genus and also M. pneumoniae species in the synovial fluid of patients with RA. The results indicated that M. pneumoniae can be a probable cause of rheumatoid arthritis disease. This finding may help the specific treatment of RA. |
| first_indexed | 2024-12-19T23:28:31Z |
| format | Article |
| id | doaj.art-f22764f09f5f4b2abc89ba6f07162b6d |
| institution | Directory Open Access Journal |
| issn | 1735-8612 2345-4342 |
| language | English |
| last_indexed | 2024-12-19T23:28:31Z |
| publishDate | 2014-06-01 |
| publisher | Farname |
| record_format | Article |
| series | Iranian Journal of Medical Microbiology |
| spelling | doaj.art-f22764f09f5f4b2abc89ba6f07162b6d2022-12-21T20:01:48ZengFarnameIranian Journal of Medical Microbiology1735-86122345-43422014-06-018118Molecular Diagnosis of Mycoplasma pneumoniae in Synovial Fluid of Rheumatoid Arthritis PatientsReza Golmohammadi0Ramezan Ali Ataee1Gholam Hossein Alishiri2Reza Mirnejad3Ali Najafi4Mehdi Tate5Davoude Esmaieli6Nematollah Jonaidi Jafari7 Department of Medical Microbiology, Faculty of Medicine, Baqiyatallah University of Medical Sciences, Tehran, Iran Department of Medical Microbiology, Faculty of Medicine, Baqiyatallah University of Medical Sciences, Tehran, Iran Department of Rheumatology, Faculty of Medicine, Baqiyatallah University of Medical Sciences, Tehran, Iran Molecular Biology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran. Molecular Biology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran Applied Virological Research, Faculty of Medicine, Baqiyatallah University of Medical Sciences, Tehran, Iran Department of Medical Microbiology, Faculty of Medicine, Baqiyatallah University of Medical Sciences, Tehran, Iran. Health Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran. Background and Aim: The role of Mycoplasma super antigens in inflammatory diseases such as rheumatoid arthritis (RA) is taken into consideration. Therefore, the aim of this study was to diagnosis of Mycoplasma pneumoniae in synovial fluid (SF) of patients with RA.Materials and Methods: In this study, 131 synovial fluid samples of patients with RA were collected during 3 years (2011- 2013). Mycoplasma pneumoniae ATCC: 29342 strain was used as control. The genome of samples is extracted separately and PCR protocol by M. pneumoniae species and mycoplasma genus identifier primers was carried out. The PCR product was confirmed by sequencing. Results: The results of this study showed that this molecular diagnosis method is able to detect M. pneumoniae in the synovial fluid of patients with RA. So that M. pneumoniae species was confirmed in 30 samples (22.9%) and Mycoplasma genus detected in the 70 sample (53.4%). Conclusions: based on the results of this study, the PCR molecular method was able to detect of the Mycoplasma genus and also M. pneumoniae species in the synovial fluid of patients with RA. The results indicated that M. pneumoniae can be a probable cause of rheumatoid arthritis disease. This finding may help the specific treatment of RA.http://www.ijmm.ir/browse.php?a_code=A-10-69-1&slc_lang=en&sid=1Mycoplasma pneumoniae PCR Rheumatoid arthritis Synovial fluid |
| spellingShingle | Reza Golmohammadi Ramezan Ali Ataee Gholam Hossein Alishiri Reza Mirnejad Ali Najafi Mehdi Tate Davoude Esmaieli Nematollah Jonaidi Jafari Molecular Diagnosis of Mycoplasma pneumoniae in Synovial Fluid of Rheumatoid Arthritis Patients Iranian Journal of Medical Microbiology Mycoplasma pneumoniae PCR Rheumatoid arthritis Synovial fluid |
| title | Molecular Diagnosis of Mycoplasma pneumoniae in Synovial Fluid of Rheumatoid Arthritis Patients |
| title_full | Molecular Diagnosis of Mycoplasma pneumoniae in Synovial Fluid of Rheumatoid Arthritis Patients |
| title_fullStr | Molecular Diagnosis of Mycoplasma pneumoniae in Synovial Fluid of Rheumatoid Arthritis Patients |
| title_full_unstemmed | Molecular Diagnosis of Mycoplasma pneumoniae in Synovial Fluid of Rheumatoid Arthritis Patients |
| title_short | Molecular Diagnosis of Mycoplasma pneumoniae in Synovial Fluid of Rheumatoid Arthritis Patients |
| title_sort | molecular diagnosis of mycoplasma pneumoniae in synovial fluid of rheumatoid arthritis patients |
| topic | Mycoplasma pneumoniae PCR Rheumatoid arthritis Synovial fluid |
| url | http://www.ijmm.ir/browse.php?a_code=A-10-69-1&slc_lang=en&sid=1 |
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