In Vitro Comparisons of Minimal Inhibitory Concentrations between NaOCL, CHX, MTAD and EDTA against Candida Albicans
The aim of this in vitro study was to evaluate the minimal inhibitory concentration (MIC) of 3% NaOCl, 2% CHX, MTAD, and EDTA against Candida albicans (C. albicans). Methods and Results: Certified strain of C. albicans (ATCC 10231 OXOID, Hampshire, UK) was used to determine the MIC of 3% NaOCl, 2%...
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| Format: | Article |
| Language: | English |
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International Medical Research and Development Corporation
2023-12-01
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| Series: | International Journal of Biomedicine |
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| Online Access: | http://www.ijbm.org/articles/i52/ijbm_13(4)_oa19.pdf |
| _version_ | 1797398936946737152 |
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| author | Donika Bajrami Shabani Agime Dragidella Teneqja |
| author_facet | Donika Bajrami Shabani Agime Dragidella Teneqja |
| author_sort | Donika Bajrami Shabani |
| collection | DOAJ |
| description | The aim of this in vitro study was to evaluate the minimal inhibitory concentration (MIC) of 3% NaOCl, 2% CHX, MTAD, and EDTA against Candida albicans (C. albicans).
Methods and Results: Certified strain of C. albicans (ATCC 10231 OXOID, Hampshire, UK) was used to determine the MIC of 3% NaOCl, 2% CHX, MTAD, and EDTA in vitro. The broth dilution method was used to determine the MIC and to ensure the test was highly accurate. C. albicans and respective irrigants were gradually placed into the appropriate test tubes, starting from 1 mL to 0.06 mL of irrigant (getting halved each time). The test tubes were incubated at 37°C for 24 h. MIC was then recorded as the lowest concentration of irrigant that inhibited microbial growth, based on based on spectrophotometry. It was found the higher the value of the absorbance rate, the smaller the antifungal effectiveness of the tested substance. Our study showed that some of the tested irrigants retained an antifungal effect after dilution, which is valuable because dilution reduces toxicity. 3% NaOCl has an efficient antifungal effect against C. albicans both at full concentration and when diluted fivefold. The antifungal effect of 2% CHX for C. albicans cultures increases with its dilution. MTAD retains a good antifungal effect even when diluted fivefold. |
| first_indexed | 2024-03-09T01:32:46Z |
| format | Article |
| id | doaj.art-f2320e9cddda4f578b374a75ab58f389 |
| institution | Directory Open Access Journal |
| issn | 2158-0510 2158-0529 |
| language | English |
| last_indexed | 2024-03-09T01:32:46Z |
| publishDate | 2023-12-01 |
| publisher | International Medical Research and Development Corporation |
| record_format | Article |
| series | International Journal of Biomedicine |
| spelling | doaj.art-f2320e9cddda4f578b374a75ab58f3892023-12-09T06:15:21ZengInternational Medical Research and Development CorporationInternational Journal of Biomedicine2158-05102158-05292023-12-0113434534910.21103/Article13(4)_OA19In Vitro Comparisons of Minimal Inhibitory Concentrations between NaOCL, CHX, MTAD and EDTA against Candida AlbicansDonika Bajrami Shabani0Agime Dragidella Teneqja1Department of Dental Pathology and Endodontics, Faculty of Medicine, University of Prishtina ''Hasan Prishtina'', Prishtina, KosovoDepartment of Dental Pathology and Endodontics, Faculty of Medicine, University of Prishtina ''Hasan Prishtina'', Prishtina, KosovoThe aim of this in vitro study was to evaluate the minimal inhibitory concentration (MIC) of 3% NaOCl, 2% CHX, MTAD, and EDTA against Candida albicans (C. albicans). Methods and Results: Certified strain of C. albicans (ATCC 10231 OXOID, Hampshire, UK) was used to determine the MIC of 3% NaOCl, 2% CHX, MTAD, and EDTA in vitro. The broth dilution method was used to determine the MIC and to ensure the test was highly accurate. C. albicans and respective irrigants were gradually placed into the appropriate test tubes, starting from 1 mL to 0.06 mL of irrigant (getting halved each time). The test tubes were incubated at 37°C for 24 h. MIC was then recorded as the lowest concentration of irrigant that inhibited microbial growth, based on based on spectrophotometry. It was found the higher the value of the absorbance rate, the smaller the antifungal effectiveness of the tested substance. Our study showed that some of the tested irrigants retained an antifungal effect after dilution, which is valuable because dilution reduces toxicity. 3% NaOCl has an efficient antifungal effect against C. albicans both at full concentration and when diluted fivefold. The antifungal effect of 2% CHX for C. albicans cultures increases with its dilution. MTAD retains a good antifungal effect even when diluted fivefold.http://www.ijbm.org/articles/i52/ijbm_13(4)_oa19.pdfcandida albicansminimal inhibitory concentrationmtadchxnaocledta |
| spellingShingle | Donika Bajrami Shabani Agime Dragidella Teneqja In Vitro Comparisons of Minimal Inhibitory Concentrations between NaOCL, CHX, MTAD and EDTA against Candida Albicans International Journal of Biomedicine candida albicans minimal inhibitory concentration mtad chx naocl edta |
| title | In Vitro Comparisons of Minimal Inhibitory Concentrations between NaOCL, CHX, MTAD and EDTA against Candida Albicans |
| title_full | In Vitro Comparisons of Minimal Inhibitory Concentrations between NaOCL, CHX, MTAD and EDTA against Candida Albicans |
| title_fullStr | In Vitro Comparisons of Minimal Inhibitory Concentrations between NaOCL, CHX, MTAD and EDTA against Candida Albicans |
| title_full_unstemmed | In Vitro Comparisons of Minimal Inhibitory Concentrations between NaOCL, CHX, MTAD and EDTA against Candida Albicans |
| title_short | In Vitro Comparisons of Minimal Inhibitory Concentrations between NaOCL, CHX, MTAD and EDTA against Candida Albicans |
| title_sort | in vitro comparisons of minimal inhibitory concentrations between naocl chx mtad and edta against candida albicans |
| topic | candida albicans minimal inhibitory concentration mtad chx naocl edta |
| url | http://www.ijbm.org/articles/i52/ijbm_13(4)_oa19.pdf |
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