Proteomic Studies on the Mechanism of Myostatin Regulating Cattle Skeletal Muscle Development
Myostatin (MSTN) is an important negative regulator of muscle growth and development. In this study, we performed comparatively the proteomics analyses of gluteus tissues from MSTN+/− Mongolian cattle (MG.MSTN+/−) and wild type Mongolian cattle (MG.WT) using a shotgun-based tandem mass tag (TMT) 6-p...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2021-11-01
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| Series: | Frontiers in Genetics |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fgene.2021.752129/full |
| _version_ | 1827978971113848832 |
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| author | Hui Sheng Yiwen Guo Linlin Zhang Junxing Zhang Manning Miao Haoyun Tan Debao Hu Xin Li Xiangbin Ding Guangpeng Li Hong Guo |
| author_facet | Hui Sheng Yiwen Guo Linlin Zhang Junxing Zhang Manning Miao Haoyun Tan Debao Hu Xin Li Xiangbin Ding Guangpeng Li Hong Guo |
| author_sort | Hui Sheng |
| collection | DOAJ |
| description | Myostatin (MSTN) is an important negative regulator of muscle growth and development. In this study, we performed comparatively the proteomics analyses of gluteus tissues from MSTN+/− Mongolian cattle (MG.MSTN+/−) and wild type Mongolian cattle (MG.WT) using a shotgun-based tandem mass tag (TMT) 6-plex labeling method to investigate the regulation mechanism of MSTN on the growth and development of bovine skeletal muscle. A total of 1,950 proteins were identified in MG.MSTN+/− and MG.WT. Compared with MG.WT cattle, a total of 320 differentially expressed proteins were identified in MG.MSTN cattle, including 245 up-regulated differentially expressed proteins and 75 down-regulated differentially expressed proteins. Bioinformatics analysis showed that knockdown of the MSTN gene increased the expression of extracellular matrix and ribosome-related proteins, induced activation of focal adhesion, PI3K-AKT, and Ribosomal pathways. The results of proteomic analysis were verified by muscle tissue Western blot test and in vitro MSTN gene knockdown test, and it was found that knockdown MSTN gene expression could promote the proliferation and myogenic differentiation of bovine skeletal muscle satellite cells (BSMSCs). At the same time, Co-Immunoprecipitation (CO-IP) assay showed that MSTN gene interacted with extracellular matrix related protein type I collagen α 1 (COL1A1), and knocking down the expression of COL1A1 could inhibit the activity of adhesion, PI3K-AKT and ribosome pathway, thus inhibit BSMSCs proliferation. These results suggest that the MSTN gene regulates focal adhesion, PI3K-AKT, and Ribosomal pathway through the COL1A1 gene. In general, this study provides new insights into the regulatory mechanism of MSTN involved in muscle growth and development. |
| first_indexed | 2024-04-09T21:29:45Z |
| format | Article |
| id | doaj.art-f232d15e3b6e471c99cd820ee956b95e |
| institution | Directory Open Access Journal |
| issn | 1664-8021 |
| language | English |
| last_indexed | 2024-04-09T21:29:45Z |
| publishDate | 2021-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Genetics |
| spelling | doaj.art-f232d15e3b6e471c99cd820ee956b95e2023-03-27T11:05:54ZengFrontiers Media S.A.Frontiers in Genetics1664-80212021-11-011210.3389/fgene.2021.752129752129Proteomic Studies on the Mechanism of Myostatin Regulating Cattle Skeletal Muscle DevelopmentHui Sheng0Yiwen Guo1Linlin Zhang2Junxing Zhang3Manning Miao4Haoyun Tan5Debao Hu6Xin Li7Xiangbin Ding8Guangpeng Li9Hong Guo10Tianjin Key Laboratory of Agricultural Animal Breeding and Healthy Husbandry, College of Animal Science and Veterinary Medicine, Tianjin Agricultural University, Tianjin, ChinaTianjin Key Laboratory of Agricultural Animal Breeding and Healthy Husbandry, College of Animal Science and Veterinary Medicine, Tianjin Agricultural University, Tianjin, ChinaTianjin Key Laboratory of Agricultural Animal Breeding and Healthy Husbandry, College of Animal Science and Veterinary Medicine, Tianjin Agricultural University, Tianjin, ChinaTianjin Key Laboratory of Agricultural Animal Breeding and Healthy Husbandry, College of Animal Science and Veterinary Medicine, Tianjin Agricultural University, Tianjin, ChinaTianjin Key Laboratory of Agricultural Animal Breeding and Healthy Husbandry, College of Animal Science and Veterinary Medicine, Tianjin Agricultural University, Tianjin, ChinaTianjin Key Laboratory of Agricultural Animal Breeding and Healthy Husbandry, College of Animal Science and Veterinary Medicine, Tianjin Agricultural University, Tianjin, ChinaTianjin Key Laboratory of Agricultural Animal Breeding and Healthy Husbandry, College of Animal Science and Veterinary Medicine, Tianjin Agricultural University, Tianjin, ChinaTianjin Key Laboratory of Agricultural Animal Breeding and Healthy Husbandry, College of Animal Science and Veterinary Medicine, Tianjin Agricultural University, Tianjin, ChinaTianjin Key Laboratory of Agricultural Animal Breeding and Healthy Husbandry, College of Animal Science and Veterinary Medicine, Tianjin Agricultural University, Tianjin, ChinaThe Key Laboratory of Mammalian Reproductive Biology and Biotechnology of the Ministry of Education, Inner Mongolia University, Hohhot, ChinaTianjin Key Laboratory of Agricultural Animal Breeding and Healthy Husbandry, College of Animal Science and Veterinary Medicine, Tianjin Agricultural University, Tianjin, ChinaMyostatin (MSTN) is an important negative regulator of muscle growth and development. In this study, we performed comparatively the proteomics analyses of gluteus tissues from MSTN+/− Mongolian cattle (MG.MSTN+/−) and wild type Mongolian cattle (MG.WT) using a shotgun-based tandem mass tag (TMT) 6-plex labeling method to investigate the regulation mechanism of MSTN on the growth and development of bovine skeletal muscle. A total of 1,950 proteins were identified in MG.MSTN+/− and MG.WT. Compared with MG.WT cattle, a total of 320 differentially expressed proteins were identified in MG.MSTN cattle, including 245 up-regulated differentially expressed proteins and 75 down-regulated differentially expressed proteins. Bioinformatics analysis showed that knockdown of the MSTN gene increased the expression of extracellular matrix and ribosome-related proteins, induced activation of focal adhesion, PI3K-AKT, and Ribosomal pathways. The results of proteomic analysis were verified by muscle tissue Western blot test and in vitro MSTN gene knockdown test, and it was found that knockdown MSTN gene expression could promote the proliferation and myogenic differentiation of bovine skeletal muscle satellite cells (BSMSCs). At the same time, Co-Immunoprecipitation (CO-IP) assay showed that MSTN gene interacted with extracellular matrix related protein type I collagen α 1 (COL1A1), and knocking down the expression of COL1A1 could inhibit the activity of adhesion, PI3K-AKT and ribosome pathway, thus inhibit BSMSCs proliferation. These results suggest that the MSTN gene regulates focal adhesion, PI3K-AKT, and Ribosomal pathway through the COL1A1 gene. In general, this study provides new insights into the regulatory mechanism of MSTN involved in muscle growth and development.https://www.frontiersin.org/articles/10.3389/fgene.2021.752129/fullmyostatinproteomicsextracellular matrixribosomefocal adhesion |
| spellingShingle | Hui Sheng Yiwen Guo Linlin Zhang Junxing Zhang Manning Miao Haoyun Tan Debao Hu Xin Li Xiangbin Ding Guangpeng Li Hong Guo Proteomic Studies on the Mechanism of Myostatin Regulating Cattle Skeletal Muscle Development Frontiers in Genetics myostatin proteomics extracellular matrix ribosome focal adhesion |
| title | Proteomic Studies on the Mechanism of Myostatin Regulating Cattle Skeletal Muscle Development |
| title_full | Proteomic Studies on the Mechanism of Myostatin Regulating Cattle Skeletal Muscle Development |
| title_fullStr | Proteomic Studies on the Mechanism of Myostatin Regulating Cattle Skeletal Muscle Development |
| title_full_unstemmed | Proteomic Studies on the Mechanism of Myostatin Regulating Cattle Skeletal Muscle Development |
| title_short | Proteomic Studies on the Mechanism of Myostatin Regulating Cattle Skeletal Muscle Development |
| title_sort | proteomic studies on the mechanism of myostatin regulating cattle skeletal muscle development |
| topic | myostatin proteomics extracellular matrix ribosome focal adhesion |
| url | https://www.frontiersin.org/articles/10.3389/fgene.2021.752129/full |
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