Investigation of Growth Factors and Mathematical Modeling of Nutrient Media for the Shoots Multiplication In Vitro of Rare Plants of the Rostov Region
Micropropagation is an effective way to preserve the gene pool of threatened plants. This study is devoted to the mathematical modeling of nutrient media and the study of the effect of <i>m</i>T (meta-topoline) on the multiplication of shoots of <i>Hedysarum grandiflorum</i>,...
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2023-01-01
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| Series: | Horticulturae |
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| author | Vasiliy A. Chokheli Semyon D. Bakulin Olga Yu. Ermolaeva Boris L. Kozlovsky Pavel A. Dmitriev Victoriya V. Stepanenko Igor V. Kornienko Anastasia A. Bushkova Vishnu D. Rajput Tatiana V. Varduny |
| author_facet | Vasiliy A. Chokheli Semyon D. Bakulin Olga Yu. Ermolaeva Boris L. Kozlovsky Pavel A. Dmitriev Victoriya V. Stepanenko Igor V. Kornienko Anastasia A. Bushkova Vishnu D. Rajput Tatiana V. Varduny |
| author_sort | Vasiliy A. Chokheli |
| collection | DOAJ |
| description | Micropropagation is an effective way to preserve the gene pool of threatened plants. This study is devoted to the mathematical modeling of nutrient media and the study of the effect of <i>m</i>T (meta-topoline) on the multiplication of shoots of <i>Hedysarum grandiflorum</i>, <i>Hyssopus cretaceus</i>, and <i>Matthiola fragrans</i> in vitro in comparison with benzylaminopurine (BAP) and kinetin (KT). Initiation was performed on an MS medium with 0.5 mg/L BAP. For shoots multiplication, MS, B5, and WPM media were used with the addition of <i>m</i>T, BAP, KT. For <i>H. grandiflorum</i>, the multiplication coefficient of shoots was highest on medium B5 with the addition of mT at a concentration of 1 mg/L—2.90 shoots per plant, for <i>H. cretaceus</i>—B5 + 0.5 mg/L <i>m</i>T, and for <i>M. fragrans</i>—B5 + 1 mg/L KT. A positive effect of <i>m</i>T on <i>H. grandiflorum</i> and <i>M. fragrans</i> in vitro was found. The efficiency of using KT for <i>H. cretaceus</i> shoot multiplication is shown. The effectiveness of the B5 nutrient medium for <i>H. grandiflorum</i> and <i>M. fragrans</i> was determined. The positive effect of WPM for <i>H. cretaceus</i> micropropagation has been demonstrated. It is not recommended to use the MS media for micropropagation of these plant species. |
| first_indexed | 2024-03-09T12:30:12Z |
| format | Article |
| id | doaj.art-f2331746c0a4495eb7c8a79f5649784a |
| institution | Directory Open Access Journal |
| issn | 2311-7524 |
| language | English |
| last_indexed | 2024-03-09T12:30:12Z |
| publishDate | 2023-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Horticulturae |
| spelling | doaj.art-f2331746c0a4495eb7c8a79f5649784a2023-11-30T22:30:20ZengMDPI AGHorticulturae2311-75242023-01-01916010.3390/horticulturae9010060Investigation of Growth Factors and Mathematical Modeling of Nutrient Media for the Shoots Multiplication In Vitro of Rare Plants of the Rostov RegionVasiliy A. Chokheli0Semyon D. Bakulin1Olga Yu. Ermolaeva2Boris L. Kozlovsky3Pavel A. Dmitriev4Victoriya V. Stepanenko5Igor V. Kornienko6Anastasia A. Bushkova7Vishnu D. Rajput8Tatiana V. Varduny9Academy of Biology and Biotechnology, Southern Federal University, Rostov on Don 344090, RussiaDepartment of Botany, Moscow Timiryazev Agricultural Academy, Russian State Agrarian University, Plant Breeding and Seed Technology, Moscow 127550, RussiaAcademy of Biology and Biotechnology, Southern Federal University, Rostov on Don 344090, RussiaAcademy of Biology and Biotechnology, Southern Federal University, Rostov on Don 344090, RussiaAcademy of Biology and Biotechnology, Southern Federal University, Rostov on Don 344090, RussiaAcademy of Biology and Biotechnology, Southern Federal University, Rostov on Don 344090, RussiaAcademy of Biology and Biotechnology, Southern Federal University, Rostov on Don 344090, RussiaAcademy of Biology and Biotechnology, Southern Federal University, Rostov on Don 344090, RussiaAcademy of Biology and Biotechnology, Southern Federal University, Rostov on Don 344090, RussiaAcademy of Biology and Biotechnology, Southern Federal University, Rostov on Don 344090, RussiaMicropropagation is an effective way to preserve the gene pool of threatened plants. This study is devoted to the mathematical modeling of nutrient media and the study of the effect of <i>m</i>T (meta-topoline) on the multiplication of shoots of <i>Hedysarum grandiflorum</i>, <i>Hyssopus cretaceus</i>, and <i>Matthiola fragrans</i> in vitro in comparison with benzylaminopurine (BAP) and kinetin (KT). Initiation was performed on an MS medium with 0.5 mg/L BAP. For shoots multiplication, MS, B5, and WPM media were used with the addition of <i>m</i>T, BAP, KT. For <i>H. grandiflorum</i>, the multiplication coefficient of shoots was highest on medium B5 with the addition of mT at a concentration of 1 mg/L—2.90 shoots per plant, for <i>H. cretaceus</i>—B5 + 0.5 mg/L <i>m</i>T, and for <i>M. fragrans</i>—B5 + 1 mg/L KT. A positive effect of <i>m</i>T on <i>H. grandiflorum</i> and <i>M. fragrans</i> in vitro was found. The efficiency of using KT for <i>H. cretaceus</i> shoot multiplication is shown. The effectiveness of the B5 nutrient medium for <i>H. grandiflorum</i> and <i>M. fragrans</i> was determined. The positive effect of WPM for <i>H. cretaceus</i> micropropagation has been demonstrated. It is not recommended to use the MS media for micropropagation of these plant species.https://www.mdpi.com/2311-7524/9/1/60red list6-benzylaminopurinemeta-topolinekinetinmicropropagationphytohormones |
| spellingShingle | Vasiliy A. Chokheli Semyon D. Bakulin Olga Yu. Ermolaeva Boris L. Kozlovsky Pavel A. Dmitriev Victoriya V. Stepanenko Igor V. Kornienko Anastasia A. Bushkova Vishnu D. Rajput Tatiana V. Varduny Investigation of Growth Factors and Mathematical Modeling of Nutrient Media for the Shoots Multiplication In Vitro of Rare Plants of the Rostov Region Horticulturae red list 6-benzylaminopurine meta-topoline kinetin micropropagation phytohormones |
| title | Investigation of Growth Factors and Mathematical Modeling of Nutrient Media for the Shoots Multiplication In Vitro of Rare Plants of the Rostov Region |
| title_full | Investigation of Growth Factors and Mathematical Modeling of Nutrient Media for the Shoots Multiplication In Vitro of Rare Plants of the Rostov Region |
| title_fullStr | Investigation of Growth Factors and Mathematical Modeling of Nutrient Media for the Shoots Multiplication In Vitro of Rare Plants of the Rostov Region |
| title_full_unstemmed | Investigation of Growth Factors and Mathematical Modeling of Nutrient Media for the Shoots Multiplication In Vitro of Rare Plants of the Rostov Region |
| title_short | Investigation of Growth Factors and Mathematical Modeling of Nutrient Media for the Shoots Multiplication In Vitro of Rare Plants of the Rostov Region |
| title_sort | investigation of growth factors and mathematical modeling of nutrient media for the shoots multiplication in vitro of rare plants of the rostov region |
| topic | red list 6-benzylaminopurine meta-topoline kinetin micropropagation phytohormones |
| url | https://www.mdpi.com/2311-7524/9/1/60 |
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