Feed Supplemented with <i>Aronia melanocarpa</i> (AM) Relieves the Oxidative Stress Caused by Ovulation in Peak Laying Hens and Increases the Content of Yolk Precursors
The continuous ovulation of laying hens during the peak period is likely to cause oxidative stress, resulting in a reduction in the laying cycle over time. The aim of this study was to evaluate the antioxidant effects of <i>Aronia melanocarpa</i> (AM) in the diet and its effect on the yo...
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MDPI AG
2022-12-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/12/24/3574 |
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| author | Bo Jing Huanwei Xiao Haixu Yin Yingbo Wei Haoyuan Wu Dongliang Zhang Ivan Stève Nguepi Tsopmejio Hongmei Shang Zhouyu Jin Hui Song |
| author_facet | Bo Jing Huanwei Xiao Haixu Yin Yingbo Wei Haoyuan Wu Dongliang Zhang Ivan Stève Nguepi Tsopmejio Hongmei Shang Zhouyu Jin Hui Song |
| author_sort | Bo Jing |
| collection | DOAJ |
| description | The continuous ovulation of laying hens during the peak period is likely to cause oxidative stress, resulting in a reduction in the laying cycle over time. The aim of this study was to evaluate the antioxidant effects of <i>Aronia melanocarpa</i> (AM) in the diet and its effect on the yolk precursor content caused by ovulation in laying hens during the peak period. A total of 300 25-week-old Roman brown laying hens were randomly divided into five groups with six replicates in each group, 10 in each replicate. The control group was fed a basal diet, the positive control group was fed a Vitamin C (VC) plus basal diet, and the experimental group was fed 1%, 4%, and 7% doses of AM plus diet according to the principle of energy and nitrogen requirements, which lasted eight weeks. At the end of the study, the egg quality, biochemical, and antioxidant markers, as well as mRNA and protein expressions, were evaluated to determine the potential signaling pathways involved. Results showed that the addition of AM to the feed increased the weight of laying hens at the peak of egg production and improved egg quality. The biochemical markers, as well as the antioxidant parameters in the serum, liver, and ovarian tissues, were ameliorated. The gene and protein expression of recombinant kelch-like ECH-associated protein 1 (Keap1) in the liver and ovarian tissues was decreased, while nuclear factor erythroid-2 related factor 2 (Nrf2) and heme oxygenase-1 (HO-1) expression was increased. The feed supplemented with AM also increased the estrogen contents and lipid parameters, as well as the gene and protein expressions related to the yolk precursor. Feed supplemented with AM could improve the egg quality and the oxidative stress caused by the ovulation process of laying hens during the peak egg production period by activating the Keap1/Nrf2 signaling pathway. These results suggest that the feed supplemented with 1% AM and 4% AM can improve egg production in peak laying hens. |
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| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-09T17:24:33Z |
| publishDate | 2022-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-f23407a3e2e345ee98bcf102d8353ee72023-11-24T12:52:08ZengMDPI AGAnimals2076-26152022-12-011224357410.3390/ani12243574Feed Supplemented with <i>Aronia melanocarpa</i> (AM) Relieves the Oxidative Stress Caused by Ovulation in Peak Laying Hens and Increases the Content of Yolk PrecursorsBo Jing0Huanwei Xiao1Haixu Yin2Yingbo Wei3Haoyuan Wu4Dongliang Zhang5Ivan Stève Nguepi Tsopmejio6Hongmei Shang7Zhouyu Jin8Hui Song9School of Life Science, Jilin Agricultural University, Changchun 130118, ChinaSchool of Life Science, Jilin Agricultural University, Changchun 130118, ChinaSchool of Life Science, Jilin Agricultural University, Changchun 130118, ChinaSchool of Life Science, Jilin Agricultural University, Changchun 130118, ChinaSchool of Life Science, Jilin Agricultural University, Changchun 130118, ChinaSchool of Life Science, Jilin Agricultural University, Changchun 130118, ChinaSchool of Life Science, Jilin Agricultural University, Changchun 130118, ChinaCollege of Animal Science and Technology, Jilin Agricultural University, Changchun 130118, ChinaSchool of Life Science, Jilin Agricultural University, Changchun 130118, ChinaSchool of Life Science, Jilin Agricultural University, Changchun 130118, ChinaThe continuous ovulation of laying hens during the peak period is likely to cause oxidative stress, resulting in a reduction in the laying cycle over time. The aim of this study was to evaluate the antioxidant effects of <i>Aronia melanocarpa</i> (AM) in the diet and its effect on the yolk precursor content caused by ovulation in laying hens during the peak period. A total of 300 25-week-old Roman brown laying hens were randomly divided into five groups with six replicates in each group, 10 in each replicate. The control group was fed a basal diet, the positive control group was fed a Vitamin C (VC) plus basal diet, and the experimental group was fed 1%, 4%, and 7% doses of AM plus diet according to the principle of energy and nitrogen requirements, which lasted eight weeks. At the end of the study, the egg quality, biochemical, and antioxidant markers, as well as mRNA and protein expressions, were evaluated to determine the potential signaling pathways involved. Results showed that the addition of AM to the feed increased the weight of laying hens at the peak of egg production and improved egg quality. The biochemical markers, as well as the antioxidant parameters in the serum, liver, and ovarian tissues, were ameliorated. The gene and protein expression of recombinant kelch-like ECH-associated protein 1 (Keap1) in the liver and ovarian tissues was decreased, while nuclear factor erythroid-2 related factor 2 (Nrf2) and heme oxygenase-1 (HO-1) expression was increased. The feed supplemented with AM also increased the estrogen contents and lipid parameters, as well as the gene and protein expressions related to the yolk precursor. Feed supplemented with AM could improve the egg quality and the oxidative stress caused by the ovulation process of laying hens during the peak egg production period by activating the Keap1/Nrf2 signaling pathway. These results suggest that the feed supplemented with 1% AM and 4% AM can improve egg production in peak laying hens.https://www.mdpi.com/2076-2615/12/24/3574<i>Aronia melanocarpa</i>peak laying hensoxidative stressegg qualityyolk precursor |
| spellingShingle | Bo Jing Huanwei Xiao Haixu Yin Yingbo Wei Haoyuan Wu Dongliang Zhang Ivan Stève Nguepi Tsopmejio Hongmei Shang Zhouyu Jin Hui Song Feed Supplemented with <i>Aronia melanocarpa</i> (AM) Relieves the Oxidative Stress Caused by Ovulation in Peak Laying Hens and Increases the Content of Yolk Precursors Animals <i>Aronia melanocarpa</i> peak laying hens oxidative stress egg quality yolk precursor |
| title | Feed Supplemented with <i>Aronia melanocarpa</i> (AM) Relieves the Oxidative Stress Caused by Ovulation in Peak Laying Hens and Increases the Content of Yolk Precursors |
| title_full | Feed Supplemented with <i>Aronia melanocarpa</i> (AM) Relieves the Oxidative Stress Caused by Ovulation in Peak Laying Hens and Increases the Content of Yolk Precursors |
| title_fullStr | Feed Supplemented with <i>Aronia melanocarpa</i> (AM) Relieves the Oxidative Stress Caused by Ovulation in Peak Laying Hens and Increases the Content of Yolk Precursors |
| title_full_unstemmed | Feed Supplemented with <i>Aronia melanocarpa</i> (AM) Relieves the Oxidative Stress Caused by Ovulation in Peak Laying Hens and Increases the Content of Yolk Precursors |
| title_short | Feed Supplemented with <i>Aronia melanocarpa</i> (AM) Relieves the Oxidative Stress Caused by Ovulation in Peak Laying Hens and Increases the Content of Yolk Precursors |
| title_sort | feed supplemented with i aronia melanocarpa i am relieves the oxidative stress caused by ovulation in peak laying hens and increases the content of yolk precursors |
| topic | <i>Aronia melanocarpa</i> peak laying hens oxidative stress egg quality yolk precursor |
| url | https://www.mdpi.com/2076-2615/12/24/3574 |
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