Epiphytic yeasts as potential antagonists against Fusarium head blight of wheat (Triticum aestivum L.) caused by Fusarium graminearum sensu stricto
Fusarium head blight (FHB), caused by Fusarium graminearum, is a significant disease of wheat in all wheat-growing areas. In this study, we screened a total of 344 yeast epiphytes isolated from cereal and weed plants against F. graminearum in vitro. Twelve potential antagonists were selected and sub...
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| Format: | Article |
| Language: | English |
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Elsevier
2022-09-01
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| Series: | Journal of the Saudi Society of Agricultural Sciences |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1658077X21001521 |
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| author | Sinegugu Precious N. Shude Nokwazi Carol Mbili Kwasi Sackey Yobo |
| author_facet | Sinegugu Precious N. Shude Nokwazi Carol Mbili Kwasi Sackey Yobo |
| author_sort | Sinegugu Precious N. Shude |
| collection | DOAJ |
| description | Fusarium head blight (FHB), caused by Fusarium graminearum, is a significant disease of wheat in all wheat-growing areas. In this study, we screened a total of 344 yeast epiphytes isolated from cereal and weed plants against F. graminearum in vitro. Twelve potential antagonists were selected and subjected to in planta screening trials. In the in vitro bioassay trials, the highest average percentage inhibition of 64.31% (Isolate MGO2) was observed. The in vivo evaluation of the best 12 isolates showed a reduction in FHB severity and deoxynivalenol (DON) concentration of up to 15.62% (Isolate WL3) and 28.96% (Isolate SCH7), respectively. We observed a positive correlation between Area Under the Disease Progress Curve (AUDPC) and percentage seed infection. The 100-seed weight, however, was negatively correlated to both the AUDPC and the percentage seed infection. The best three yeast antagonists were selected based on AUDPC units, percentage seed infection, and 100-seed weight parameters and were identified as Papiliotrema flavescens (Isolates WL3 and WL6) and Pseudozyma sp. (Isolate MGO1) using 16S rRNA sequence analysis. These three yeast antagonists showed biocontrol efficacy against F. graminearum, and their incorporation in an integrated management programme could provide enhanced FHB control. To the best of our knowledge, this is the first report of yeast epiphytes isolated from Panicum maximum, and Ophiopogon japonicus tested as potential biological control agents against F. graminearum. |
| first_indexed | 2024-04-13T18:24:04Z |
| format | Article |
| id | doaj.art-f2347bcec2c544fc98728108979b6028 |
| institution | Directory Open Access Journal |
| issn | 1658-077X |
| language | English |
| last_indexed | 2024-04-13T18:24:04Z |
| publishDate | 2022-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of the Saudi Society of Agricultural Sciences |
| spelling | doaj.art-f2347bcec2c544fc98728108979b60282022-12-22T02:35:19ZengElsevierJournal of the Saudi Society of Agricultural Sciences1658-077X2022-09-01216404411Epiphytic yeasts as potential antagonists against Fusarium head blight of wheat (Triticum aestivum L.) caused by Fusarium graminearum sensu strictoSinegugu Precious N. Shude0Nokwazi Carol Mbili1Kwasi Sackey Yobo2Discipline of Plant Pathology, School of Agricultural, Earth and Environmental Sciences, University of KwaZulu-Natal, Scottsville, Pietermaritzburg, Republic of South AfricaDiscipline of Plant Pathology, School of Agricultural, Earth and Environmental Sciences, University of KwaZulu-Natal, Scottsville, Pietermaritzburg, Republic of South AfricaCorresponding author at: Discipline of Plant Pathology, School of Agricultural, Earth and Environmental Sciences, University of KwaZulu-Natal, Private Bag X01, Scottsville, Pietermaritzburg 3209. Republic of South Africa.; Discipline of Plant Pathology, School of Agricultural, Earth and Environmental Sciences, University of KwaZulu-Natal, Scottsville, Pietermaritzburg, Republic of South AfricaFusarium head blight (FHB), caused by Fusarium graminearum, is a significant disease of wheat in all wheat-growing areas. In this study, we screened a total of 344 yeast epiphytes isolated from cereal and weed plants against F. graminearum in vitro. Twelve potential antagonists were selected and subjected to in planta screening trials. In the in vitro bioassay trials, the highest average percentage inhibition of 64.31% (Isolate MGO2) was observed. The in vivo evaluation of the best 12 isolates showed a reduction in FHB severity and deoxynivalenol (DON) concentration of up to 15.62% (Isolate WL3) and 28.96% (Isolate SCH7), respectively. We observed a positive correlation between Area Under the Disease Progress Curve (AUDPC) and percentage seed infection. The 100-seed weight, however, was negatively correlated to both the AUDPC and the percentage seed infection. The best three yeast antagonists were selected based on AUDPC units, percentage seed infection, and 100-seed weight parameters and were identified as Papiliotrema flavescens (Isolates WL3 and WL6) and Pseudozyma sp. (Isolate MGO1) using 16S rRNA sequence analysis. These three yeast antagonists showed biocontrol efficacy against F. graminearum, and their incorporation in an integrated management programme could provide enhanced FHB control. To the best of our knowledge, this is the first report of yeast epiphytes isolated from Panicum maximum, and Ophiopogon japonicus tested as potential biological control agents against F. graminearum.http://www.sciencedirect.com/science/article/pii/S1658077X21001521Biological controlCereal cropDeoxynivalenolFusarium graminearumPanicum maximumYeast |
| spellingShingle | Sinegugu Precious N. Shude Nokwazi Carol Mbili Kwasi Sackey Yobo Epiphytic yeasts as potential antagonists against Fusarium head blight of wheat (Triticum aestivum L.) caused by Fusarium graminearum sensu stricto Journal of the Saudi Society of Agricultural Sciences Biological control Cereal crop Deoxynivalenol Fusarium graminearum Panicum maximum Yeast |
| title | Epiphytic yeasts as potential antagonists against Fusarium head blight of wheat (Triticum aestivum L.) caused by Fusarium graminearum sensu stricto |
| title_full | Epiphytic yeasts as potential antagonists against Fusarium head blight of wheat (Triticum aestivum L.) caused by Fusarium graminearum sensu stricto |
| title_fullStr | Epiphytic yeasts as potential antagonists against Fusarium head blight of wheat (Triticum aestivum L.) caused by Fusarium graminearum sensu stricto |
| title_full_unstemmed | Epiphytic yeasts as potential antagonists against Fusarium head blight of wheat (Triticum aestivum L.) caused by Fusarium graminearum sensu stricto |
| title_short | Epiphytic yeasts as potential antagonists against Fusarium head blight of wheat (Triticum aestivum L.) caused by Fusarium graminearum sensu stricto |
| title_sort | epiphytic yeasts as potential antagonists against fusarium head blight of wheat triticum aestivum l caused by fusarium graminearum sensu stricto |
| topic | Biological control Cereal crop Deoxynivalenol Fusarium graminearum Panicum maximum Yeast |
| url | http://www.sciencedirect.com/science/article/pii/S1658077X21001521 |
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