Effect of the Crude Extract of Coprophilous Fungi on Some Bacterial Species Isolated from Cases of Mastitis
Coprophilous fungi are a large group of fungi mostly found in herbivore dung and have an exclusive life cycle. This group of fungi produces many important metabolites which can be consumed in medicine or agriculture. The present study aimed to investigate the antibacterial effects of these fungi on...
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Razi Vaccine and Serum Research Institute
2021-11-01
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| Series: | Archives of Razi Institute |
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| Online Access: | https://archrazi.areeo.ac.ir/article_125179_6a70bc2e9cc3d7d61c8aa71afafddb58.pdf |
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| author | A. S Jasim B. A Abass I. M Al-Rubayae |
| author_facet | A. S Jasim B. A Abass I. M Al-Rubayae |
| author_sort | A. S Jasim |
| collection | DOAJ |
| description | Coprophilous fungi are a large group of fungi mostly found in herbivore dung and have an exclusive life cycle. This group of fungi produces many important metabolites which can be consumed in medicine or agriculture. The present study aimed to investigate the antibacterial effects of these fungi on bacterial mastitis. A total of 50 dung samples were collected from four herbivores (cows, buffalos, sheep, and camels) from different areas of Basra. The moist chamber method was used for each sample to establish a fungal fruiting body and detect the type of the fungi. The coprophilous fungi included Aspergillus sp. (A. niger, A. fumigatus, A. flavus, A. terrus), Chaetomium sp., Sordaria sp., and Podospora sp. which belong to the Ascomycetes class. PCR test was performed using the ITS region for confirmatory detection of species. The highest and the lowest number of isolated species was associated with cow dung and camel dung, respectively. The antimicrobial property of three different partitioned extracts (petroleum ether [F1], ethanol [F2], and water [F3]) prepared from some fungal mycelia was evaluated in vitro. All fractions were tested to detect antimicrobial activity using the disc diffusion assay against five pathogenic bacteria Staphylococcus aureus, Streptococcus Enterobacter, Proteus mirabilis, and E. coli. which is isolated from bovine mastitis. Data revealed that all fractions could inhibit the tested bacteria. However, inhibitory activity was found to be dependent on (6i) the used fungal strains; (ii) the extracted solvent; and (iii) the tested bacteria. In general, the petroleum ether extracts (F1) derived from all fungi displayed the highest inhibitory activity against the testing bacteria. In conclusion, the present study concluded that the extracts prepared from the fungal mycelia contain bioactive compounds with antibacterial properties. This study was first conducted in Iraq and further studies are required to develop new treatments. |
| first_indexed | 2024-12-11T05:47:12Z |
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| institution | Directory Open Access Journal |
| issn | 0365-3439 2008-9872 |
| language | English |
| last_indexed | 2024-12-11T05:47:12Z |
| publishDate | 2021-11-01 |
| publisher | Razi Vaccine and Serum Research Institute |
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| series | Archives of Razi Institute |
| spelling | doaj.art-f235e87ac5aa47cbb1a2d77c1b639b222022-12-22T01:18:56ZengRazi Vaccine and Serum Research InstituteArchives of Razi Institute0365-34392008-98722021-11-017651333134110.22092/ari.2021.356366.1832125179Effect of the Crude Extract of Coprophilous Fungi on Some Bacterial Species Isolated from Cases of MastitisA. S Jasim0B. A Abass1I. M Al-Rubayae2Department of Microbiology and Parasitology, College of Veterinary Medicine, University of Basra, Basrah, IraqDepartment of Microbiology and Parasitology, College of Veterinary Medicine, University of Basra, Basrah, IraqDepartment of Biology, College of Science, University of Basra, Basrah, IraqCoprophilous fungi are a large group of fungi mostly found in herbivore dung and have an exclusive life cycle. This group of fungi produces many important metabolites which can be consumed in medicine or agriculture. The present study aimed to investigate the antibacterial effects of these fungi on bacterial mastitis. A total of 50 dung samples were collected from four herbivores (cows, buffalos, sheep, and camels) from different areas of Basra. The moist chamber method was used for each sample to establish a fungal fruiting body and detect the type of the fungi. The coprophilous fungi included Aspergillus sp. (A. niger, A. fumigatus, A. flavus, A. terrus), Chaetomium sp., Sordaria sp., and Podospora sp. which belong to the Ascomycetes class. PCR test was performed using the ITS region for confirmatory detection of species. The highest and the lowest number of isolated species was associated with cow dung and camel dung, respectively. The antimicrobial property of three different partitioned extracts (petroleum ether [F1], ethanol [F2], and water [F3]) prepared from some fungal mycelia was evaluated in vitro. All fractions were tested to detect antimicrobial activity using the disc diffusion assay against five pathogenic bacteria Staphylococcus aureus, Streptococcus Enterobacter, Proteus mirabilis, and E. coli. which is isolated from bovine mastitis. Data revealed that all fractions could inhibit the tested bacteria. However, inhibitory activity was found to be dependent on (6i) the used fungal strains; (ii) the extracted solvent; and (iii) the tested bacteria. In general, the petroleum ether extracts (F1) derived from all fungi displayed the highest inhibitory activity against the testing bacteria. In conclusion, the present study concluded that the extracts prepared from the fungal mycelia contain bioactive compounds with antibacterial properties. This study was first conducted in Iraq and further studies are required to develop new treatments.https://archrazi.areeo.ac.ir/article_125179_6a70bc2e9cc3d7d61c8aa71afafddb58.pdfantibiotic activity testcoprophilous fungiherbivore dungiraq |
| spellingShingle | A. S Jasim B. A Abass I. M Al-Rubayae Effect of the Crude Extract of Coprophilous Fungi on Some Bacterial Species Isolated from Cases of Mastitis Archives of Razi Institute antibiotic activity test coprophilous fungi herbivore dung iraq |
| title | Effect of the Crude Extract of Coprophilous Fungi on Some Bacterial Species Isolated from Cases of Mastitis |
| title_full | Effect of the Crude Extract of Coprophilous Fungi on Some Bacterial Species Isolated from Cases of Mastitis |
| title_fullStr | Effect of the Crude Extract of Coprophilous Fungi on Some Bacterial Species Isolated from Cases of Mastitis |
| title_full_unstemmed | Effect of the Crude Extract of Coprophilous Fungi on Some Bacterial Species Isolated from Cases of Mastitis |
| title_short | Effect of the Crude Extract of Coprophilous Fungi on Some Bacterial Species Isolated from Cases of Mastitis |
| title_sort | effect of the crude extract of coprophilous fungi on some bacterial species isolated from cases of mastitis |
| topic | antibiotic activity test coprophilous fungi herbivore dung iraq |
| url | https://archrazi.areeo.ac.ir/article_125179_6a70bc2e9cc3d7d61c8aa71afafddb58.pdf |
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