Identification, evolution, expression and protein interaction analysis of genes encoding B-box zinc-finger proteins in maize
The B-box (BBX) family of proteins consists of zinc-finger transcription factors with one or two highly conserved B-box motifs at their N-termini. BBX proteins play crucial roles in various aspects of plant growth and development, including seedling photomorphogenesis, shade avoidance, flowering tim...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-02-01
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| Series: | Journal of Integrative Agriculture |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2095311922001691 |
| _version_ | 1828052352255393792 |
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| author | Xiao-hui XU Wen-lan LI Shu-ke YANG Xiang-zhen ZHU Hong-wei SUN Fan LI Xing-bo LU Jin-jie CUI |
| author_facet | Xiao-hui XU Wen-lan LI Shu-ke YANG Xiang-zhen ZHU Hong-wei SUN Fan LI Xing-bo LU Jin-jie CUI |
| author_sort | Xiao-hui XU |
| collection | DOAJ |
| description | The B-box (BBX) family of proteins consists of zinc-finger transcription factors with one or two highly conserved B-box motifs at their N-termini. BBX proteins play crucial roles in various aspects of plant growth and development, including seedling photomorphogenesis, shade avoidance, flowering time, and biotic and abiotic stress responses. Previous studies have identified many different BBXs from several plant species, although the BBX family members in maize are largely unknown. Genome-wide identification and comprehensive analysis of maize BBX (ZmBBX) expression and interaction networks would therefore provide valuable information for understanding their functions. In this study, 36 maize BBXs in three major clades were identified. The ZmBBXs within a given clade were found to share similar domains, motifs, and genomic structures. Gene duplication analyses revealed that the expansion of BBX proteins in maize has mainly occurred by segmental duplication. The expression levels of ZmBBXs were analyzed in various organs and tissues, and under different abiotic stress conditions. Protein–protein interaction networks of ZmBBXs were established using bioinformatic tools and verified by bimolecular fluorescence complementation (BiFC) assays. Our findings can facilitate a greater understanding of the complexity of the ZmBBX family and provide novel clues for unravelling ZmBBX protein functions. |
| first_indexed | 2024-04-10T19:52:19Z |
| format | Article |
| id | doaj.art-f235f4d87df84d6296c072e775142c24 |
| institution | Directory Open Access Journal |
| issn | 2095-3119 |
| language | English |
| last_indexed | 2024-04-10T19:52:19Z |
| publishDate | 2023-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Integrative Agriculture |
| spelling | doaj.art-f235f4d87df84d6296c072e775142c242023-01-28T04:07:38ZengElsevierJournal of Integrative Agriculture2095-31192023-02-01222371388Identification, evolution, expression and protein interaction analysis of genes encoding B-box zinc-finger proteins in maizeXiao-hui XU0Wen-lan LI1Shu-ke YANG2Xiang-zhen ZHU3Hong-wei SUN4Fan LI5Xing-bo LU6Jin-jie CUI7Shandong Key Laboratory of Plant Virology/Institute of Plant Protection, Shandong Academy of Agricultural Sciences, Jinan 250100, P.R.ChinaMaize Research Institute, Shandong Academy of Agricultural Sciences, Jinan 250100, P.R.ChinaShandong Key Laboratory of Plant Virology/Institute of Plant Protection, Shandong Academy of Agricultural Sciences, Jinan 250100, P.R.ChinaState Key Laboratory of Cotton Biology/Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang 455000, P.R.ChinaShandong Key Laboratory of Plant Virology/Institute of Plant Protection, Shandong Academy of Agricultural Sciences, Jinan 250100, P.R.ChinaShandong Key Laboratory of Plant Virology/Institute of Plant Protection, Shandong Academy of Agricultural Sciences, Jinan 250100, P.R.ChinaShandong Key Laboratory of Plant Virology/Institute of Plant Protection, Shandong Academy of Agricultural Sciences, Jinan 250100, P.R.China; Correspondence LU Xing-boState Key Laboratory of Cotton Biology/Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang 455000, P.R.China; Correspondence CUI Jin-jieThe B-box (BBX) family of proteins consists of zinc-finger transcription factors with one or two highly conserved B-box motifs at their N-termini. BBX proteins play crucial roles in various aspects of plant growth and development, including seedling photomorphogenesis, shade avoidance, flowering time, and biotic and abiotic stress responses. Previous studies have identified many different BBXs from several plant species, although the BBX family members in maize are largely unknown. Genome-wide identification and comprehensive analysis of maize BBX (ZmBBX) expression and interaction networks would therefore provide valuable information for understanding their functions. In this study, 36 maize BBXs in three major clades were identified. The ZmBBXs within a given clade were found to share similar domains, motifs, and genomic structures. Gene duplication analyses revealed that the expansion of BBX proteins in maize has mainly occurred by segmental duplication. The expression levels of ZmBBXs were analyzed in various organs and tissues, and under different abiotic stress conditions. Protein–protein interaction networks of ZmBBXs were established using bioinformatic tools and verified by bimolecular fluorescence complementation (BiFC) assays. Our findings can facilitate a greater understanding of the complexity of the ZmBBX family and provide novel clues for unravelling ZmBBX protein functions.http://www.sciencedirect.com/science/article/pii/S2095311922001691maizeB-box family proteinevolutionexpressionprotein interaction |
| spellingShingle | Xiao-hui XU Wen-lan LI Shu-ke YANG Xiang-zhen ZHU Hong-wei SUN Fan LI Xing-bo LU Jin-jie CUI Identification, evolution, expression and protein interaction analysis of genes encoding B-box zinc-finger proteins in maize Journal of Integrative Agriculture maize B-box family protein evolution expression protein interaction |
| title | Identification, evolution, expression and protein interaction analysis of genes encoding B-box zinc-finger proteins in maize |
| title_full | Identification, evolution, expression and protein interaction analysis of genes encoding B-box zinc-finger proteins in maize |
| title_fullStr | Identification, evolution, expression and protein interaction analysis of genes encoding B-box zinc-finger proteins in maize |
| title_full_unstemmed | Identification, evolution, expression and protein interaction analysis of genes encoding B-box zinc-finger proteins in maize |
| title_short | Identification, evolution, expression and protein interaction analysis of genes encoding B-box zinc-finger proteins in maize |
| title_sort | identification evolution expression and protein interaction analysis of genes encoding b box zinc finger proteins in maize |
| topic | maize B-box family protein evolution expression protein interaction |
| url | http://www.sciencedirect.com/science/article/pii/S2095311922001691 |
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