Saccharomyces paradoxus K66 Killer System Evidences Expanded Assortment of Helper and Satellite Viruses
The Saccharomycetaceae yeast family recently became recognized for expanding of the repertoire of different dsRNA-based viruses, highlighting the need for understanding of their cross-dependence. We isolated the Saccharomyces paradoxus AML-15-66 killer strain from spontaneous fermentation of service...
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MDPI AG
2018-10-01
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| Series: | Viruses |
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| Online Access: | http://www.mdpi.com/1999-4915/10/10/564 |
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| author | Iglė Vepštaitė-Monstavičė Juliana Lukša Aleksandras Konovalovas Dovilė Ežerskytė Ramunė Stanevičienė Živilė Strazdaitė-Žielienė Saulius Serva Elena Servienė |
| author_facet | Iglė Vepštaitė-Monstavičė Juliana Lukša Aleksandras Konovalovas Dovilė Ežerskytė Ramunė Stanevičienė Živilė Strazdaitė-Žielienė Saulius Serva Elena Servienė |
| author_sort | Iglė Vepštaitė-Monstavičė |
| collection | DOAJ |
| description | The Saccharomycetaceae yeast family recently became recognized for expanding of the repertoire of different dsRNA-based viruses, highlighting the need for understanding of their cross-dependence. We isolated the Saccharomyces paradoxus AML-15-66 killer strain from spontaneous fermentation of serviceberries and identified helper and satellite viruses of the family Totiviridae, which are responsible for the killing phenotype. The corresponding full dsRNA genomes of viruses have been cloned and sequenced. Sequence analysis of SpV-LA-66 identified it to be most similar to S. paradoxus LA-28 type viruses, while SpV-M66 was mostly similar to the SpV-M21 virus. Sequence and functional analysis revealed significant differences between the K66 and the K28 toxins. The structural organization of the K66 protein resembled those of the K1/K2 type toxins. The AML-15-66 strain possesses the most expressed killing property towards the K28 toxin-producing strain. A genetic screen performed on S. cerevisiae YKO library strains revealed 125 gene products important for the functioning of the S. paradoxus K66 toxin, with 85% of the discovered modulators shared with S. cerevisiae K2 or K1 toxins. Investigation of the K66 protein binding to cells and different polysaccharides implies the β-1,6 glucans to be the primary receptors of S. paradoxus K66 toxin. For the first time, we demonstrated the coherent habitation of different types of helper and satellite viruses in a wild-type S. paradoxus strain. |
| first_indexed | 2024-04-12T10:28:45Z |
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| id | doaj.art-f2362bbf12924fec94c3fd5a664a5504 |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-04-12T10:28:45Z |
| publishDate | 2018-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-f2362bbf12924fec94c3fd5a664a55042022-12-22T03:36:55ZengMDPI AGViruses1999-49152018-10-01101056410.3390/v10100564v10100564Saccharomyces paradoxus K66 Killer System Evidences Expanded Assortment of Helper and Satellite VirusesIglė Vepštaitė-Monstavičė0Juliana Lukša1Aleksandras Konovalovas2Dovilė Ežerskytė3Ramunė Stanevičienė4Živilė Strazdaitė-Žielienė5Saulius Serva6Elena Servienė7Laboratory of Genetics, Institute of Botany, Nature Research Centre, LT-08412 Vilnius, LithuaniaLaboratory of Genetics, Institute of Botany, Nature Research Centre, LT-08412 Vilnius, LithuaniaDepartment of Biochemistry and Molecular Biology, Institute of Biosciences, Vilnius University, LT-10257 Vilnius, LithuaniaLaboratory of Genetics, Institute of Botany, Nature Research Centre, LT-08412 Vilnius, LithuaniaLaboratory of Genetics, Institute of Botany, Nature Research Centre, LT-08412 Vilnius, LithuaniaLaboratory of Genetics, Institute of Botany, Nature Research Centre, LT-08412 Vilnius, LithuaniaDepartment of Biochemistry and Molecular Biology, Institute of Biosciences, Vilnius University, LT-10257 Vilnius, LithuaniaLaboratory of Genetics, Institute of Botany, Nature Research Centre, LT-08412 Vilnius, LithuaniaThe Saccharomycetaceae yeast family recently became recognized for expanding of the repertoire of different dsRNA-based viruses, highlighting the need for understanding of their cross-dependence. We isolated the Saccharomyces paradoxus AML-15-66 killer strain from spontaneous fermentation of serviceberries and identified helper and satellite viruses of the family Totiviridae, which are responsible for the killing phenotype. The corresponding full dsRNA genomes of viruses have been cloned and sequenced. Sequence analysis of SpV-LA-66 identified it to be most similar to S. paradoxus LA-28 type viruses, while SpV-M66 was mostly similar to the SpV-M21 virus. Sequence and functional analysis revealed significant differences between the K66 and the K28 toxins. The structural organization of the K66 protein resembled those of the K1/K2 type toxins. The AML-15-66 strain possesses the most expressed killing property towards the K28 toxin-producing strain. A genetic screen performed on S. cerevisiae YKO library strains revealed 125 gene products important for the functioning of the S. paradoxus K66 toxin, with 85% of the discovered modulators shared with S. cerevisiae K2 or K1 toxins. Investigation of the K66 protein binding to cells and different polysaccharides implies the β-1,6 glucans to be the primary receptors of S. paradoxus K66 toxin. For the first time, we demonstrated the coherent habitation of different types of helper and satellite viruses in a wild-type S. paradoxus strain.http://www.mdpi.com/1999-4915/10/10/564Saccharomyces paradoxusTotiviridaedsRNA viruskiller system |
| spellingShingle | Iglė Vepštaitė-Monstavičė Juliana Lukša Aleksandras Konovalovas Dovilė Ežerskytė Ramunė Stanevičienė Živilė Strazdaitė-Žielienė Saulius Serva Elena Servienė Saccharomyces paradoxus K66 Killer System Evidences Expanded Assortment of Helper and Satellite Viruses Viruses Saccharomyces paradoxus Totiviridae dsRNA virus killer system |
| title | Saccharomyces paradoxus K66 Killer System Evidences Expanded Assortment of Helper and Satellite Viruses |
| title_full | Saccharomyces paradoxus K66 Killer System Evidences Expanded Assortment of Helper and Satellite Viruses |
| title_fullStr | Saccharomyces paradoxus K66 Killer System Evidences Expanded Assortment of Helper and Satellite Viruses |
| title_full_unstemmed | Saccharomyces paradoxus K66 Killer System Evidences Expanded Assortment of Helper and Satellite Viruses |
| title_short | Saccharomyces paradoxus K66 Killer System Evidences Expanded Assortment of Helper and Satellite Viruses |
| title_sort | saccharomyces paradoxus k66 killer system evidences expanded assortment of helper and satellite viruses |
| topic | Saccharomyces paradoxus Totiviridae dsRNA virus killer system |
| url | http://www.mdpi.com/1999-4915/10/10/564 |
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