In Vitro and Ex Situ Biodegradation of Low-Density Polyethylene by a Rhizopus sp. Strain Isolated from a Local Dumpsite in North-East Algeria
Low density polyethylene (LDPE) is the most abundant non-degradable plastic waste. Widely used in packaging material, it represents a serious threat to all ecosystems. In the present study, a Rhizopus sp. fungal strain was isolated from soil of a landfill located in north-east Algeria and cultured o...
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| Format: | Article |
| Language: | English |
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Mahidol University
2022-09-01
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| Series: | Environment and Natural Resources Journal |
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| Online Access: | https://ph02.tci-thaijo.org/index.php/ennrj/article/view/246868/167392 |
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| author | Randa Harrat Ghania Bourzama Houria Ouled-Haddar Boudjema Soumati |
| author_facet | Randa Harrat Ghania Bourzama Houria Ouled-Haddar Boudjema Soumati |
| author_sort | Randa Harrat |
| collection | DOAJ |
| description | Low density polyethylene (LDPE) is the most abundant non-degradable plastic waste. Widely used in packaging material, it represents a serious threat to all ecosystems. In the present study, a Rhizopus sp. fungal strain was isolated from soil of a landfill located in north-east Algeria and cultured on potato dextrose agar. The in vitro biodegradability of pieces of the same plastic bag (0.2, 0.4, and 0.6 g) was estimated in minimal liquid medium and on minimal solid medium. Furthermore, biodegradation of plastic bag pieces was examined in seawater, tap water and soil. The isolated Rhizopus sp. strain could degrade the plastic bag waste. The highest in vitro rate occurred in the minimal liquid medium for both the 0.4-g and 0.6-g pieces (a 20% decrease in weight). In natural media, the highest weight decrease was different depending on the substrate: 5% in seawater for the 0.2-g piece, 10% in tap water for the 0.4-g piece and 8% in soil for the 0.4-g piece. This strain could also form a biofilm in Malt Extract Broth (MEB). These results revealed that the isolated Rhizopus sp. strain has considerable biodegradative ability based on different measures. |
| first_indexed | 2024-04-12T08:06:13Z |
| format | Article |
| id | doaj.art-f2410422f0704fc5afe926338e318f27 |
| institution | Directory Open Access Journal |
| issn | 1686-5456 2408-2384 |
| language | English |
| last_indexed | 2024-04-12T08:06:13Z |
| publishDate | 2022-09-01 |
| publisher | Mahidol University |
| record_format | Article |
| series | Environment and Natural Resources Journal |
| spelling | doaj.art-f2410422f0704fc5afe926338e318f272022-12-22T03:41:11ZengMahidol UniversityEnvironment and Natural Resources Journal1686-54562408-23842022-09-0120546547410.32526/ennrj/20/202200026In Vitro and Ex Situ Biodegradation of Low-Density Polyethylene by a Rhizopus sp. Strain Isolated from a Local Dumpsite in North-East AlgeriaRanda Harrat0Ghania Bourzama1Houria Ouled-Haddar2Boudjema Soumati3Laboratory of Biochemistry and Environmental Toxicology, Department of Biochemistry, Faculty of Sciences, University of Badji Mokhtar, Annaba 23000, AlgeriaLaboratory of Biochemistry and Environmental Toxicology, Department of Biochemistry, Faculty of Sciences, University of Badji Mokhtar, Annaba 23000, AlgeriaLaboratory of Molecular Toxicology, University of Jijel, Jijel, Ouled Aïssa 18000, AlgeriaLaboratory of Biochemistry and Environmental Toxicology, Department of Biochemistry, Faculty of Sciences, University of Badji Mokhtar, Annaba 23000, AlgeriaLow density polyethylene (LDPE) is the most abundant non-degradable plastic waste. Widely used in packaging material, it represents a serious threat to all ecosystems. In the present study, a Rhizopus sp. fungal strain was isolated from soil of a landfill located in north-east Algeria and cultured on potato dextrose agar. The in vitro biodegradability of pieces of the same plastic bag (0.2, 0.4, and 0.6 g) was estimated in minimal liquid medium and on minimal solid medium. Furthermore, biodegradation of plastic bag pieces was examined in seawater, tap water and soil. The isolated Rhizopus sp. strain could degrade the plastic bag waste. The highest in vitro rate occurred in the minimal liquid medium for both the 0.4-g and 0.6-g pieces (a 20% decrease in weight). In natural media, the highest weight decrease was different depending on the substrate: 5% in seawater for the 0.2-g piece, 10% in tap water for the 0.4-g piece and 8% in soil for the 0.4-g piece. This strain could also form a biofilm in Malt Extract Broth (MEB). These results revealed that the isolated Rhizopus sp. strain has considerable biodegradative ability based on different measures.https://ph02.tci-thaijo.org/index.php/ennrj/article/view/246868/167392biodegradationfungal strainplastic bagpolyethylenerhizopus sp. |
| spellingShingle | Randa Harrat Ghania Bourzama Houria Ouled-Haddar Boudjema Soumati In Vitro and Ex Situ Biodegradation of Low-Density Polyethylene by a Rhizopus sp. Strain Isolated from a Local Dumpsite in North-East Algeria Environment and Natural Resources Journal biodegradation fungal strain plastic bag polyethylene rhizopus sp. |
| title | In Vitro and Ex Situ Biodegradation of Low-Density Polyethylene by a Rhizopus sp. Strain Isolated from a Local Dumpsite in North-East Algeria |
| title_full | In Vitro and Ex Situ Biodegradation of Low-Density Polyethylene by a Rhizopus sp. Strain Isolated from a Local Dumpsite in North-East Algeria |
| title_fullStr | In Vitro and Ex Situ Biodegradation of Low-Density Polyethylene by a Rhizopus sp. Strain Isolated from a Local Dumpsite in North-East Algeria |
| title_full_unstemmed | In Vitro and Ex Situ Biodegradation of Low-Density Polyethylene by a Rhizopus sp. Strain Isolated from a Local Dumpsite in North-East Algeria |
| title_short | In Vitro and Ex Situ Biodegradation of Low-Density Polyethylene by a Rhizopus sp. Strain Isolated from a Local Dumpsite in North-East Algeria |
| title_sort | in vitro and ex situ biodegradation of low density polyethylene by a rhizopus sp strain isolated from a local dumpsite in north east algeria |
| topic | biodegradation fungal strain plastic bag polyethylene rhizopus sp. |
| url | https://ph02.tci-thaijo.org/index.php/ennrj/article/view/246868/167392 |
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