Virtual histology of transgenic mouse embryos for high-throughput phenotyping.
A bold new effort to disrupt every gene in the mouse genome necessitates systematic, interdisciplinary approaches to analyzing patterning defects in the mouse embryo. We present a novel, rapid, and inexpensive method for obtaining high-resolution virtual histology for phenotypic assessment of mouse...
| Format: | Article |
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| Language: | English |
| Published: |
Public Library of Science (PLoS)
2006-04-01
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| Series: | PLoS Genetics |
| Online Access: | http://dx.doi.org/10.1371/journal.pgen.0020061 |
| _version_ | 1819264701865918464 |
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| collection | DOAJ |
| description | A bold new effort to disrupt every gene in the mouse genome necessitates systematic, interdisciplinary approaches to analyzing patterning defects in the mouse embryo. We present a novel, rapid, and inexpensive method for obtaining high-resolution virtual histology for phenotypic assessment of mouse embryos. Using osmium tetroxide to differentially stain tissues followed by volumetric X-ray computed tomography to image whole embryos, isometric resolutions of 27 mum or 8 mum were achieved with scan times of 2 h or 12 h, respectively, using mid-gestation E9.5-E12.5 embryos. The datasets generated by this method are immediately amenable to state-of-the-art computational methods of organ patterning analysis. This technique to assess embryo anatomy represents a significant improvement in resolution, time, and expense for the quantitative, three-dimensional analysis of developmental patterning defects attributed to genetically engineered mutations and chemically induced embryotoxicity. |
| first_indexed | 2024-12-23T20:33:40Z |
| format | Article |
| id | doaj.art-f24247d5b81b46fa9f6f3cc8a5914cb7 |
| institution | Directory Open Access Journal |
| issn | 1553-7390 1553-7404 |
| language | English |
| last_indexed | 2024-12-23T20:33:40Z |
| publishDate | 2006-04-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Genetics |
| spelling | doaj.art-f24247d5b81b46fa9f6f3cc8a5914cb72022-12-21T17:32:09ZengPublic Library of Science (PLoS)PLoS Genetics1553-73901553-74042006-04-0124e61Virtual histology of transgenic mouse embryos for high-throughput phenotyping.A bold new effort to disrupt every gene in the mouse genome necessitates systematic, interdisciplinary approaches to analyzing patterning defects in the mouse embryo. We present a novel, rapid, and inexpensive method for obtaining high-resolution virtual histology for phenotypic assessment of mouse embryos. Using osmium tetroxide to differentially stain tissues followed by volumetric X-ray computed tomography to image whole embryos, isometric resolutions of 27 mum or 8 mum were achieved with scan times of 2 h or 12 h, respectively, using mid-gestation E9.5-E12.5 embryos. The datasets generated by this method are immediately amenable to state-of-the-art computational methods of organ patterning analysis. This technique to assess embryo anatomy represents a significant improvement in resolution, time, and expense for the quantitative, three-dimensional analysis of developmental patterning defects attributed to genetically engineered mutations and chemically induced embryotoxicity.http://dx.doi.org/10.1371/journal.pgen.0020061 |
| spellingShingle | Virtual histology of transgenic mouse embryos for high-throughput phenotyping. PLoS Genetics |
| title | Virtual histology of transgenic mouse embryos for high-throughput phenotyping. |
| title_full | Virtual histology of transgenic mouse embryos for high-throughput phenotyping. |
| title_fullStr | Virtual histology of transgenic mouse embryos for high-throughput phenotyping. |
| title_full_unstemmed | Virtual histology of transgenic mouse embryos for high-throughput phenotyping. |
| title_short | Virtual histology of transgenic mouse embryos for high-throughput phenotyping. |
| title_sort | virtual histology of transgenic mouse embryos for high throughput phenotyping |
| url | http://dx.doi.org/10.1371/journal.pgen.0020061 |