Morphological and molecular identification of <i>Neomoliniella longicorpa</i> gen. et sp. nov. (Digenea: Echinostomatidae) from the Eurasian coot <i>Fulica atra</i> (Aves: Rallidae): a taxonomic evaluation
The purpose of the research is the morphological and molecular identification of trematodes found in the intestines of migratory waterfowl with subsequent taxonomic evaluation.Materials and methods. Mature specimens of trematodes were collected from the intestines of waterfowl (Fulica atra and Aythy...
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| Format: | Article |
| Language: | Russian |
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Federal Scientific Centre VIEV
2023-06-01
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| Series: | Российский паразитологический журнал |
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| Online Access: | https://vniigis.elpub.ru/jour/article/view/1036 |
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| author | K. S. Vainutis A. N. Voronova M. E. Andreev M. Yu. Shchelkanov |
| author_facet | K. S. Vainutis A. N. Voronova M. E. Andreev M. Yu. Shchelkanov |
| author_sort | K. S. Vainutis |
| collection | DOAJ |
| description | The purpose of the research is the morphological and molecular identification of trematodes found in the intestines of migratory waterfowl with subsequent taxonomic evaluation.Materials and methods. Mature specimens of trematodes were collected from the intestines of waterfowl (Fulica atra and Aythya ferina) shot during the ecological-faunistic monitoring in the southern part of the Primorsky krai. Preliminary morphological analyze revealed that trematodes belong to the Echinostomatidae family. The whole-mounts of worms from F. atra were prepared using standard methods with alum carmine. Divergent domains of 28S ribosomal RNA gene were sequenced and used to determine systematic position and relationships of discovered species of echinostome by reconstructing Bayesian and Maximum likelihood trees.Results and discussion. Trematodes found in the coots' intestine, should be described as Neomoliniella longicorpa in the new genus Neomoliniella gen. et sp. nov., which is reliably confirmed by morphological and genetics data. Another cryptic echinostomatid species sister to Neomoliniella gen. nov., and probably representing the separate unknown genus, was demonstrated only on the phylogenetic reconstructions and confirmed by large genetic distances. Currently, this taxon is indicated as Echinostomatidae gen. sp. and more evidence is needed for its validation. We also report about the first detection of Echinostoma paraulum from A. ferina in the south of the Russian Far East. The biology, morphology of each life cycle stage of newly described species of worms should be investigated to test not only their scientific but also medical significance. For the first time in the history of the group, dichotomous keys for eight families of the superfamilyEchinostomatoidea and, to date, the largest of all previously known for the family Echinostomatidae identification chart – dichotomous keys for the identification of 27 Palearctic genera were compiled. |
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| institution | Directory Open Access Journal |
| issn | 1998-8435 2541-7843 |
| language | Russian |
| last_indexed | 2024-04-25T02:04:34Z |
| publishDate | 2023-06-01 |
| publisher | Federal Scientific Centre VIEV |
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| series | Российский паразитологический журнал |
| spelling | doaj.art-f242647ca3f64f05951a265a352cf2a32024-03-07T14:34:32ZrusFederal Scientific Centre VIEVРоссийский паразитологический журнал1998-84352541-78432023-06-0117218119710.31016/1998-8435-2023-17-2-181-197742Morphological and molecular identification of <i>Neomoliniella longicorpa</i> gen. et sp. nov. (Digenea: Echinostomatidae) from the Eurasian coot <i>Fulica atra</i> (Aves: Rallidae): a taxonomic evaluationK. S. Vainutis0A. N. Voronova1M. E. Andreev2M. Yu. Shchelkanov3Pacific Branch of the Federal State Budget Scientific Institution «Russian Federal Research Institute of Fisheries and Oceanography»; Water Bioresources and Aquaculture Department, Fishery and Aquaculture Institute, The Far Eastern State Technical Fisheries University (FESTFU)Pacific Branch of the Federal State Budget Scientific Institution «Russian Federal Research Institute of Fisheries and Oceanography»Pacific Branch of the Federal State Budget Scientific Institution «Russian Federal Research Institute of Fisheries and Oceanography»; Far Eastern Federal UniversityFar Eastern Federal University; G. P. Somov Research Institute of Epidemiology and Microbiology, Russian Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing; Federal Scientific Center of the East Asia Terrestrial Biodiversity, Far Eastern Branch of the RASThe purpose of the research is the morphological and molecular identification of trematodes found in the intestines of migratory waterfowl with subsequent taxonomic evaluation.Materials and methods. Mature specimens of trematodes were collected from the intestines of waterfowl (Fulica atra and Aythya ferina) shot during the ecological-faunistic monitoring in the southern part of the Primorsky krai. Preliminary morphological analyze revealed that trematodes belong to the Echinostomatidae family. The whole-mounts of worms from F. atra were prepared using standard methods with alum carmine. Divergent domains of 28S ribosomal RNA gene were sequenced and used to determine systematic position and relationships of discovered species of echinostome by reconstructing Bayesian and Maximum likelihood trees.Results and discussion. Trematodes found in the coots' intestine, should be described as Neomoliniella longicorpa in the new genus Neomoliniella gen. et sp. nov., which is reliably confirmed by morphological and genetics data. Another cryptic echinostomatid species sister to Neomoliniella gen. nov., and probably representing the separate unknown genus, was demonstrated only on the phylogenetic reconstructions and confirmed by large genetic distances. Currently, this taxon is indicated as Echinostomatidae gen. sp. and more evidence is needed for its validation. We also report about the first detection of Echinostoma paraulum from A. ferina in the south of the Russian Far East. The biology, morphology of each life cycle stage of newly described species of worms should be investigated to test not only their scientific but also medical significance. For the first time in the history of the group, dichotomous keys for eight families of the superfamilyEchinostomatoidea and, to date, the largest of all previously known for the family Echinostomatidae identification chart – dichotomous keys for the identification of 27 Palearctic genera were compiled.https://vniigis.elpub.ru/jour/article/view/1036echinostomatidae28s rrna genetaxonomykeysrussian far east |
| spellingShingle | K. S. Vainutis A. N. Voronova M. E. Andreev M. Yu. Shchelkanov Morphological and molecular identification of <i>Neomoliniella longicorpa</i> gen. et sp. nov. (Digenea: Echinostomatidae) from the Eurasian coot <i>Fulica atra</i> (Aves: Rallidae): a taxonomic evaluation Российский паразитологический журнал echinostomatidae 28s rrna gene taxonomy keys russian far east |
| title | Morphological and molecular identification of <i>Neomoliniella longicorpa</i> gen. et sp. nov. (Digenea: Echinostomatidae) from the Eurasian coot <i>Fulica atra</i> (Aves: Rallidae): a taxonomic evaluation |
| title_full | Morphological and molecular identification of <i>Neomoliniella longicorpa</i> gen. et sp. nov. (Digenea: Echinostomatidae) from the Eurasian coot <i>Fulica atra</i> (Aves: Rallidae): a taxonomic evaluation |
| title_fullStr | Morphological and molecular identification of <i>Neomoliniella longicorpa</i> gen. et sp. nov. (Digenea: Echinostomatidae) from the Eurasian coot <i>Fulica atra</i> (Aves: Rallidae): a taxonomic evaluation |
| title_full_unstemmed | Morphological and molecular identification of <i>Neomoliniella longicorpa</i> gen. et sp. nov. (Digenea: Echinostomatidae) from the Eurasian coot <i>Fulica atra</i> (Aves: Rallidae): a taxonomic evaluation |
| title_short | Morphological and molecular identification of <i>Neomoliniella longicorpa</i> gen. et sp. nov. (Digenea: Echinostomatidae) from the Eurasian coot <i>Fulica atra</i> (Aves: Rallidae): a taxonomic evaluation |
| title_sort | morphological and molecular identification of i neomoliniella longicorpa i gen et sp nov digenea echinostomatidae from the eurasian coot i fulica atra i aves rallidae a taxonomic evaluation |
| topic | echinostomatidae 28s rrna gene taxonomy keys russian far east |
| url | https://vniigis.elpub.ru/jour/article/view/1036 |
| work_keys_str_mv | AT ksvainutis morphologicalandmolecularidentificationofineomoliniellalongicorpaigenetspnovdigeneaechinostomatidaefromtheeurasiancootifulicaatraiavesrallidaeataxonomicevaluation AT anvoronova morphologicalandmolecularidentificationofineomoliniellalongicorpaigenetspnovdigeneaechinostomatidaefromtheeurasiancootifulicaatraiavesrallidaeataxonomicevaluation AT meandreev morphologicalandmolecularidentificationofineomoliniellalongicorpaigenetspnovdigeneaechinostomatidaefromtheeurasiancootifulicaatraiavesrallidaeataxonomicevaluation AT myushchelkanov morphologicalandmolecularidentificationofineomoliniellalongicorpaigenetspnovdigeneaechinostomatidaefromtheeurasiancootifulicaatraiavesrallidaeataxonomicevaluation |